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  • 1990-1994  (2)
  • 3-carbethoxypsoralen  (1)
  • 8-methoxypsoralen  (1)
  • Cell age  (1)
  • Oxidative stress
  • 1
    Electronic Resource
    Electronic Resource
    The DNA repair gene PSO3 of Saccharomyces cerevisiae belongs to the RAD3 epistasis group (1992)
    Springer
    Current genetics 21 (1992), S. 85-90 
    Details
    ISSN: 1432-0983
    Keywords: pso and rad mutants ; Repair ; S. cerevisiae ; 8-methoxypsoralen ; 3-carbethoxypsoralen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The mutant allele pso3-1 of Saccharomyces cerevisiae confers sensitivity to treatment with UV365nm (UVA) light-activated mono- and bi-functional psoralens. When pso3-1 is combined in double mutants with selected rad and pso mutant alleles and subjected to 8-MOP+UVA treatment, epistatic interaction with regard to survival is observed with pso1, pso2, and rad3. With the same treatment the combination of pso3-1 with rad6 and rad52 leads to synergistic interaction. For the monofunctional agent 3-carbethoxypsoralen (3-CPs) the analysis of double mutants yields the same results as with the bifunctional 8-methoxypsoralen (8-MOP) with the exception of the pso1-1pso3-1 double mutant. Here we find an additive interaction, i.e., the sensitivities of both parental strains are summed in the double mutant, which indicates a different substrate specificity of the repair activity encoded by the PSO1 and PSO3 genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00318660
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    A ten-minute protocol for transforming Saccharomyces cerevisiae by electroporation (1992)
    Springer
    Current genetics 22 (1992), S. 335-336 
    Details
    ISSN: 1432-0983
    Keywords: Yeast ; Rapid transformation ; Cell age
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We present a simplified and rapid method for the transformation of yeast cells by electroporation. Stationary cells, scraped off the agar of Petri dish cultures stored in the refrigerator for up to 6 weeks, are suspended in sorbitol buffer, spun down by gentle centrifugation, transferred into the electroporation cuvette, and immediately subjected to transformation via electroporation. Transformation efficiency of this 10-min method, which does not require the preparation of cell cultures, is about 10% of the hitherto best performing transformation procedure using cells of defined growth phase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00317931
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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