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  • 1990-1994  (3)
  • Convection  (1)
  • Histamine H1-receptor  (1)
  • IgG-sensitized latex  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Histamine H1-receptor of vascular endothelial cells cultured from guinea pig skin (1990)
    Springer
    Archives of dermatological research 282 (1990), S. 115-119 
    Details
    ISSN: 1432-069X
    Keywords: Histamine H1-receptor ; [3H]Mepyramine binding ; Vascular endothelial cell ; Guinea pig skin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In this study, a new procedure for the cultivation of dermal vascular endothelial cells from guinea pig ear lobe was established, and specific binding of [3H]mepyramine to the cultured cell membranes was demonstrated. The vascular endothelial cells were successfully isolated from the dermis of guinea pig ear lobes by the intravenous injection of dispase. Guinea pig serum, as a supplementary component in culture medium, is more suitable for the culture of guinea pig dermal vascular endothelial cell than is fetal calf serum. The [3H]mepyramine binding study indicated the presence of a large number of H1-receptors on the membrane of dermal vascular endothelial cells of guinea pigs, suggesting the important role of endothelial cells in cutaneous reactions to histamine.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00493469
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Preparation of latex sensitized with rabbit IgG antibody for slide reversed passive agglutination (1992)
    Springer
    Veterinary research communications 16 (1992), S. 265-272 
    Details
    ISSN: 1573-7446
    Keywords: agglutination ; IgG-sensitized latex ; lactoferrin ; reversed passive latex agglutination ; RPLA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A method is described for preparing latex particles sensitized with IgG antibody (IgG-sensitized latex) applicable to the slide reversed passive agglutination (RPLA) test. Soap-free latex (latex) was sensitized with IgG which had been isolated from rabbit anti-bovine lactoferrin serum using protein A Sepharose CL 4B. Unadsorbed protein-binding sites on the surface of latex were blocked with bovine serum albumin (BSA). IgG-sensitized latex that gave better agglutination in RPLA could be selectively obtained by centrifugation at 19 900g for 15 min in 0.01 mol/L glycine buffer (pH 7.3; specific gravity 1.042) containing 3% NaCl, 5% saccharose and 2% choline chloride. By dispersing this IgG-sensitized latex in 0.01 mol/L glycine buffer (pH 7.3) containing 1–2% BSA, a uniformly suspended, highly reactive, readily agglutinable preparation was obtained.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01839325
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Pinning and long-time-scale behavior in traveling-wave convection (1991)
    Springer
    Journal of statistical physics 64 (1991), S. 903-912 
    Details
    ISSN: 1572-9613
    Keywords: Convection ; traveling waves ; pattern formation and dynamics ; binary fluid mixtures
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract We study nonlinear traveling-wave (TW) and stationary states of convection in experiments in ethanol-water mixtures. While the TW phase velocity as a function of Rayleigh number has been recently shown to be in agreement with the predictions of theory and numerical calculations, we find that this velocity is temporally modulated at frequencies corresponding to the travel time of a single convection roll and of a roll pair past a point stationary in the convection cell. This modulation could be due to the pinning of the convection pattern by experimental inhomogeneities. For large Rayleigh numbers where stationary overturning convection is expected, we sometimes observe extremely slow unidirectional TW states. For larger Rayleigh numbers, this slow TW state starts and stops intermittently on a characteristic time scale of several days. The possible origin of these phenomena and their potential utility are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01048803
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    Paper (German National Licenses)
    Fulltext
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