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Comparative behaviour of L-929 fibroblastic and human endothelial cells onto crosslinked protein substrates (1990)

Warocquier-Clérout, R. ; Lee, Y. S. ; Penhoat, J. ; [et al.]

Springer

Cytotechnology 3 (1990), S. 259-269

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ISSN: 1573-0778

Keywords: crosslinked albumin gelatin ; cytocompatibility ; endothelial cells ; fibroblastic cell

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology

Notes: Abstract Studies were carried out to compare the behaviour of human umbilical vein endothelial cells (HUVEC) and L-929 fibroblastic cells towards proteins crosslinked by glutaraldehyde (GTA) or carbodiimide (CDI) proposed for coating of vascular prostheses. CDI crosslinking of bovine serum albumin used alone, or mixed with gelatin, allowed higher rates of cell growth and DNA synthesis than GTA crosslinking independent of cells. Assessment of the plating efficiency revealed a similar behaviour of both cells towards membranes and reference plastic surface in terms of percentages of bound cells. HUVEC proliferation onto CDI crosslinked gelatin and/or albumin membranes did not differ significantly whereas the growth of L-929 was enhanced onto gelatin albumin membranes in comparison with both gelatin membranes and the reference surface. The analysis of DNA synthesis corroborated the results of the growth curves and elicited a delay of the growth phases in HUVEC cultured onto CDI crosslinked membranes, unlike the L-929 fibroblast.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00365490

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A time integration algorithm for elasto-viscoplastic cubic crystals applied to modelling polycrystalline deformation (1992)

Maniatty, A. M. ; Dawson, P. R. ; Lee, Y. -S.

Chichester [u.a.] : Wiley-Blackwell

International Journal for Numerical Methods in Engineering 35 (1992), S. 1565-1588

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ISSN: 0029-5981

Keywords: Engineering ; Engineering General

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Mathematics , Technology

Notes: An algorithm for integrating the constitutive equations for an elasto-viscoplastic cubic crystal is presented which is shown to be easily employed in a polycrystalliné analysis. Anisotropic elastic behaviour is incorporated into the standard constitutive equations for ductile single crystals. The algorithm is shown to be efficient, robust and general. The primary advantage of this algorithm is that is provides an implicit integration of the plastic deformation gradient while including the elastic response. This permits taking large time steps while maintaining accuracy and stability. Several polycrystalline examples are presented to demonstrate the effect of the time step on the solution. Examples also are presented which compare the algorithm described herein to an algorithm which neglects the elastic part of the deformation. In addition, the effect of the anisotropic component of the elasticity is investigated by comparing the results with those obtained assuming isotropic elasticity.

Additional Material: 20 Ill.