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  • 1
    ISSN: 1432-0789
    Keywords: Phosphomonoesterase ; Phosphodiesterase ; Phosphotriesterase ; Inorganic pyrophosphatase ; Forest soils
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We investigated different types of phosphatase activity (phosphomono-, phosphodi-, phosphotriesterase, inorganic pyrophosphatase) in five forest soils in Vorarlberg, Austria. Phosphatase activity was determined both in soils and in soil extracts prepared with different solutions (distilled water, 0.1M sodium pyrophosphate at pH 7, 0.1M sodium phosphate buffer/1M KCl at pH 6.5, and a modified universal buffer at pH 4, 6.5, 9, and 11). High phosphomonoesterase activity in these soils indicated a severe deficiency in available P. Acidic phosphomonoesterase prevailed over alkaline phosphomonoesterase activity. Phosphodiesterase was highest in the least acidic soil but no general trend towards an optimum pH was recognized. Phosphotriesterase activity was observed in only two of the five soils and favoured an alkaline optimum pH; this activity was not detected in strongly acid soils. Inorganic pyrophosphatase activity was high in soils with no phosphotriesterase. Phosphomonoesterase, phosphodiesterase and inorganic pyrophosphatase activities were much lower in soil extracts than in soils.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Biology and fertility of soils 11 (1991), S. 261-266 
    ISSN: 1432-0789
    Keywords: Forest soil ; Fertilization ; Enzymes ; Lime ; Amelioration ; Microbial biomass ; Protease activity ; Phosphatase activity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In pot experiments, lime and mineral salts were applied to forest soils taken from three different sites. Microbial biomass, basal respiration, N turnover, and the activities of protease, phosphatase, and CM-cellulase were determined monthly. The fertilized soils showed a great increase in biomass, respiration and N turnover, and some increase in protease activity, whereas phosphatase activity decreased in comparison with the untreated soils. Estimates of the number of colony-forming units of different classes of microorganisms showed an increase in treated soils in most cases. The untreated soils had the lowest biological activity but the maximum application of lime and mineral salts did not automatically lead to maximum activity.
    Type of Medium: Electronic Resource
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