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  • 1990-1994  (2)
  • Immunohistochemistry  (2)
  • Transcription  (1)
  • 1
    ISSN: 1432-2307
    Keywords: T-cell receptors ; Malignant lymphomas ; Immunohistochemistry ; Transcription ; Rearrangement
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The expression of T-cell receptors (TCR) in malignant lymphomas was examined immunohistochemically by monoclonal antibodies which react with the TCRΒ or TCRδ chain. TCRΒ was expressed in 16 out of 47 non-Hodgkin's lymphomas. These included 15 T-cell lymphomas and 1 Ki-1 lymphoma. The anti-TCRΒ chain antibody,ΒF1, did not react with 26 B-cell lymphomas, 1 Ki-1 lymphoma or 6 Hodgkin's disease. The anti-TCRδ chain antibody, TCRδ1, did not react with any type of malignant lymphoma. Although TCRΒ and CD3 were co-expressed in normal lymphoid tissues and most T-cell lymphomas, 3 cases of CD3+CD4+ CD8−T-cell lymphoma failed to express TCR0. TCRΒ and Ig JH gene configurations in malignant lymphomas were examined by Southern hybridization. Although each of 9 T-cell lymphomas had a rearranged TCRΒ locus, TCRΒ gene rearrangement in the 3 cases ofΒF1−CD3+T-cell lymphomas was demonstrated by Southern blot. No transcripts of the TCRΒ gene could be found in 2 out of the 3ΒF1−CD3+T-cell lymphomas by Northern blot, indicating the lack of TCRΒ protein expression to be due to non-transcription of the TCR gene. Loss of TCRΒ proteins in these T-cell lymphomas is thus quite likely to be associated with T-cell tumour activation and progression, since 3ΒF1−CD3+T-cell lymphomas expressed CD25 (interleukin-2 receptor) to a high degree.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-1335
    Keywords: p53 ; Pyrimidine dimer ; Immunohistochemistry ; Solar keratosis ; UV
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract In order to find biomarkers to measure the effects of UV irradiation, we examined the accumulation of p53 protein and pyrimidine dimers in 18 solar keratosis specimens. Frozen or AMeX-fixed solar keratosis specimens were immunohistochemically stained by anti-p53 mouse monoclonal antibody, pAb1801 and polyclonal anti-(pyrimidine dimer) antibody. Nuclear accumulation of p53 protein was found in 5/18 (28%) solar keratosis lesions. The percentage of cases showing nuclear p53 protein varied according to the histological type; in the bowenoid type it was 4/7 (57%); in the atrophic type it was 1/7 (14%). Nuclear pyrimidine dimers were not stained in solar keratosis, although the skin of UV-irradiated nude mice was positive. Accumulation of p53 protein is a good marker for early precancerous change caused by UV exposure.
    Type of Medium: Electronic Resource
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