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  • 1
    Electronic Resource
    Electronic Resource
    Colocalization of growth hormone (GH) and glycoprotein subunit a in GH-producing pituitary adenomas in acromegalic patients (1994)
    Springer
    Acta neuropathologica 87 (1994), S. 568-571 
    Details
    ISSN: 1432-0533
    Keywords: KeyWordsAcromegaly ; Pituitary gland ; Growth hormone ; Gonadotropin α-subunit
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Thirty-one consecutive cases of pituitary adenoma in acromegalic patients were studied by immunohistochemistry. All adenomas contained cells immunoreactive with the anti-α-subunit of gonadotropic hormones (α; 0.6 – 53   % of tumor cells) as well as with anti-growth hormone (GH; 4 – 74   % of tumor cells). In serial section study, most cells immunoreactive with anti-α were identical to cells immunoreactive with anti-GH. There was a positive correlation between the percentages of cells immunoreactive for α in GH cells [α(%)/GH(%)] and those for prolactin (PRL) in immunoreactive tumor cells ♪PRL(%)/[PRL(%)+GH(%)]♪ in mixed GH cell-PRL cell adenomas, suggesting that the α-subunit may play a role in emergence of PRL cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00293316
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  • 2
    Electronic Resource
    Electronic Resource
    Immunohistochemical analysis of 61 pituitary adenomas with a monoclonal antibody to the neuron-specific β-tubulin isotype (1993)
    Springer
    Acta neuropathologica 86 (1993), S. 518-520 
    Details
    ISSN: 1432-0533
    Keywords: Neuron-specific β-tubulin ; TUJ1 monoclonal antibody ; Pituitary gland ; Pituitary adenoma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Pituitary adenomas surgically resected from 61 consecutive patients and 9 normal pituitary glands were studied by immunohistochemistry to determine the localization of the class IIIβ-tubulin isotype (neuron-specific) which is recognized by the monoclonal antibody TUJ1. In normal pituitary glands only a few cells were weakly immunopositive for TUJ1, whereas, in 43(73%) of 61 adenomas, more than 5% of tumor cells were immunopositive. The result may indicate that this neuron-specific β-tubulin isotype may be either expressed de novo or enhanced under the transformation of pituitary acinar cells to tumors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00228589
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  • 3
    Electronic Resource
    Electronic Resource
    Ultrastructure, immunohistochemistry and hormone release of pituitary adenomas in relation to prolactin production (1980)
    Springer
    Virchows Archiv 387 (1980), S. 31-46 
    Details
    ISSN: 1432-2307
    Keywords: Pituitary adenoma ; Prolactin ; Ultrastructure ; Immunohistochemistry ; Morphometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Fifteen cases of pituitary adenoma, 14 of which were associated with hyperprolactinemia, were studied by observation and granule morphometry of electron micrographs, immunohistochemistry and sequential observation of in vitro release with regard to hormone production, storage and secretion. Adenoma cells of 6 cases with marked elevation of plasma prolactin were sparsely granulated, showed characteristic ultrastrucures including the presence of small secretory granules, well developed Golgi and rough membranes, misplaced exocytosis, and positive or negative immunostaining for prolactin. These adenomas also showed vigorous release of the hormone into the circulation and/or culture medium. In vitro studies showed that negative immunostaining of adenoma cells did not preclude the production and secretion of the hormone. One densely granulated adenoma containing cells with numerous lactotroph type granules showed moderate release of prolactin into the circulation. In an acromegalic case associated with both high plasma growth hormone and prolactin, some cells were shown by immunohistochemistry to store both hormones. There were 4 adenomas which could not be shown to produce, store and secrete prolactin by any method available.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00428427
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  • 4
    Electronic Resource
    Electronic Resource
    Pancreatic endocrine tumours associated with WDHA syndrome (1985)
    Springer
    Virchows Archiv 405 (1985), S. 311-323 
    Details
    ISSN: 1432-2307
    Keywords: Endocrine pancreatic tumours ; WDHA syndrome ; Peptide hormones ; Immunostaining
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Nine pancreatic endocrine tumours of patients with watery diarrhoea hypokalaemia achlorhydria (WDHA) syndrome were examined by immunohistochemistry and electron microscopy. All cases revealed neoplastic proliferation of VIP (vasoactive intestinal peptide)-immunoreactive (IR) cells. Immunoreactivity to a novel peptide hormone PHM-27, which is processed from a common big precursor peptide of VIP (prepro VIP/PHM-27), was identified in VIP-IR cells of 8 tumours. VIP-PHM-IR cells had secretory granules measuring about 130 to 220 nm in diameter. Radioimmunoassay of tumour tissue extracts showed high VIP and PHM contents in proportional amounts in most cases. According to the results of immunostaining, the 8 tumours fell into two large groups; 5 with PP (pancreatic polypeptide)-IR cells and 3 with CT (calcitonin)-IR cells. The former group demonstrated VIP cells and PP cells intermingled in various proportions, including one tumour in which coexistence of PP-IR and VIP-IR in the same cells was demonstrated. Cell heterogeneity of the tumours and possible relationships of VIP, PP and CT cells were discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00710067
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  • 5
    Electronic Resource
    Electronic Resource
    Immunohistochemical examination of lung cancers using monoclonal antibodies reacting with sialosylated Lewisx and sialosylated Lewisa (1987)
    Springer
    Virchows Archiv 410 (1987), S. 253-261 
    Details
    ISSN: 1432-2307
    Keywords: Lung cancer ; Sialylated Lex ; Sialylated Lea ; Monoclonal antibody ; Immunohistochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In order to explore the relationship between the expression of cancer-associated glycolipids such as sialylated Lex (SLEX) and sialylated Lea (SLEA) and the histological subtypes of lung cancers, 30 cases of small cell carcinoma (SCC) and 47 cases of non-small cell carcinoma (non-SCC) were examined immunohistochemically using monoclonal antibodies reacting with SLEX and SLEA. The forty-seven cases of non-SCC included 20 cases of adenocarcinoma, 20 of squamous cell carcinoma and 7 of large cell carcinoma. Tumour cells of most non-SCCs expressed SLEX and SLEA. In adenocarcinomas, the number of tumour cells having SLEX and SLEA was more than that of squamous cell carcinomas, large cell carcinomas and SCC. In SCC, 14 of the 30 cases were found to be positive for both antigens. Although the cancer cells of 11 cases of 17 intermediate cell type SCC had both antigens, the cells of only 3 of 13 oat cell tumours expressed SLEX and SLEA. The present study shows that SLEX and SLEA are useful markers for lung adenocarcinomas, that most cases of intermediate cell type of SCCs have characteristics similar to non-SCC but that many oat cell tumours lack them.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00710832
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  • 6
    Electronic Resource
    Electronic Resource
    Production of gastrin releasing peptide by medullary carcinoma of the thyroid (1983)
    Springer
    Virchows Archiv 401 (1983), S. 99-107 
    Details
    ISSN: 1432-2307
    Keywords: Thyroid medullary carcinoma ; C-cell ; Gastrin releasing peptide ; Calcitonin ; Immunostaining
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Forty medullary carcinomas of the thyroid (MCT) with documented calcitonin (CT) production were studied immunohistochemically for the production of gastrin releasing peptide (GRP), a mammalian counterpart of amphibian bombesin. GRP-positive cells, revealed by an unlabelled peroxidase-antiperoxidase immunoenzyme histochemistry were found in 81% (34/40) of the MCTs. Variable numbers of tumor cells in positive MCTs were immunostained for GRP. In 3 cases with Sipple's syndrome, cells in scattered microscopic MCT nodules and hyperplastic intrafollicular C cells of the thyroid were frequently positive for GRP as well as for CT. Non-neoplastic C cells (or CT-positive cells) of the human thyroids were also positive for GRP. In the neoplastic and non-neoplastic C cell system, some cells were confirmed to be immunoreactive with both anti-GRP and anti-CT. All these findings indicate that GRP and CT are closely associated peptide hormones produced by the C cell system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00644793
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  • 7
    Electronic Resource
    Electronic Resource
    Human cytomegalovirus infection in foci of Langerhans cell histiocytosis (1999)
    Springer
    Virchows Archiv 434 (1999), S. 109-115 
    Details
    ISSN: 1432-2307
    Keywords: Key words Langerhans cell histiocytosis ; Cytomegalovirus ; In situ hybridization ; PCR ; Cytokine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Langerhans cell histiocytosis (LCH) has been thought to be a disorder of immune regulation, and increasingly, evidence showing that the tissue damage in LCH involves lymphokines and pro-inflammatory cytokines is reported. We detected human cytomegalovirus (HCMV)-DNA in LCH cells in the foci of LCH lesions by immunohistochemistry, in situ hybridization and PCR. HCMV was detected in the nuclei and/or cytoplasm of LCH cells in 9 of 27 LCH cases by immunostaining. HCMV was probably an early antigen. In situ hybridization revealed signals for HCMV-DNA only in the nuclei of LCH cells in 10 of the 27 LCH cases. PCR analysis was performed in 20 of the LCH cases, and HCMV-DNA was detected in 7 of these. All 7 positive cases were also positive for HCMV by ISH and IHC. These findings suggested that early phase infection or reactivation of HCMV occurred in the LCH lesions. HCMV infection may be accompanied by impaired cytokine production. Our study also suggested a relationship between HCMV infection and expression of TNFα. In tissues affected by LCH, dermatopathic lymphadenopathy or malignant fibrous histiocytoma and in normal tissues no signals for Epstein-Barr virus-RNA were detected. These findings suggest that in some cases LCH is associated with HCMV infection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004280050313
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  • 8
    Electronic Resource
    Electronic Resource
    Peptide hormone production by adenocarcinomas of the lung; its morphologic basis and histogenetic considerations (1983)
    Springer
    Virchows Archiv 400 (1983), S. 245-257 
    Details
    ISSN: 1432-2307
    Keywords: Lung cancer ; Adenocarcinoma ; Peptide hormone ; Immunostaining
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The cell source of peptide hormone production and the morphological differentiation were investigated in 18 adenocarcinomas of the lung by immunohistochemistry and/or by electron microscopy. These tumors were found by radioimmunoassay of tumor extracts to contain either one or more of 7 peptide hormones, i.e. adrenocorticotropin (ACTH),β- andγ-melanocyte stimulating hormones (MSH), somatostatin (SS), vasoactive intestinal polypeptide (VIP), gastrin releasing peptide (GRP) and calcitonin(CT). In a combined adeno- and small cell carcinoma, a considerable number of small tumor cells were positively stained for ACTH,β- andγ-MSHS and GRP. In a poorly differentiated adenocarcinoma with mucin and CT production, these products were localized in some single cells. Electron microscopy revealed secretory granules indistinguishable from exocrine or endocrine types. In another mucin-positive adenocarcinoma with high SS and CT contents, some tumor cells were stained for SS and/or CT. Two distinct exocrine and endocrine type secretory granules were found in the same cells. In tumors with 100 ng or less of the peptides/g tissue, most tumor cells were not stained for the peptides but a small number showed proportion of the adenocarcinomas of the lung may show heterogeneous differentiation in both endocrine and exocrine directions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00612186
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  • 9
    Electronic Resource
    Electronic Resource
    S-100 protein-positive Langerhans cells in various human lung cancers, especially in peripheral adenocarcinomas (1985)
    Springer
    Virchows Archiv 407 (1985), S. 177-189 
    Details
    ISSN: 1432-2307
    Keywords: S-100 protein ; Langerhans cells ; Lung cancer ; Adenocarcinoma ; Immunohistochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The appearance of S-100 protein-positive Langerhans cells was studied in 90 cases of various lung cancers by an immunohistochemical method. S-100 protein-positive dendritic cells were frequently observed in many adenocarcinomas, especially in those subclassified as bronchiolar cell or type II alveolar cell type. However, no S-100 protein-positive cells were found in “goblet cell type” adenocarcinoma. In some cases of squamous cell carcinoma and large cell carcinoma, these dendritic cells were also observed though they were fewer in number. In all cases of small cell carcinoma, however, S-100 protein-positive dendritic cells were rare. Electron microscopic study of two adenocarcinomas clearly demonstrated many Birbeck granules in the cytoplasm of S-100 protein-positive dendritic cells and confirmed that S-100 protein-positive cells in lung cancer were identical with Langerhans cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00737075
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  • 10
    Electronic Resource
    Electronic Resource
    Sialosylated lewis x expression in CD30-positive anaplastic large-cell lymphomas (1992)
    Springer
    Journal of cancer research and clinical oncology 119 (1992), S. 87-90 
    Details
    ISSN: 1432-1335
    Keywords: Sialosylated Lewis x ; Anaplastic large-cell lymphoma ; Lymphoma with NK phenotype
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The expression of sialosylated Lewis x (SLEX), a ligand for endothelial leukocyte adhesion molecule 1 in malignant lymphomas, was immunohistochemically examined, using the monoclonal antibody, CSLEX1, which specifically reacts with SLEX. It was expressed in 6 out of 64 non-Hodgkin's lymphomas, which consisted of 1 nasal large-cell lymphoma and 5 of 8 (62%) Ki-1-positive anaplastic large-cell lymphomas (ALCL). One nasal lymphoma positive for SLEX co-expressed a T cell marker, cluster of differentiation (CD) 5, and natural killer (NK) cell markers such as CD56 and CD16, indicating that SLEX+ nasal lymphoma cells are possibly malignant counterparts of SLEX+ NK cells. SLEX did not react with 30 B cell lymphomas or most Hodgkin's disease lymphomas, though it did with one lymphocyte predominance type. Although SLEX+ ALCL exhibit T cell markers in some cases, some ALCL expressing SLEX may represent histiocytic differentiation of the neoplastic cells. The lymphoma cells of ALCL were preferentially positive for SLEX, in contrast to Hodgkin's disease cells, and thus CSLEX1 in conjunction, with CD30 and CD15 should be of use for analyzing and making differential diagnoses of routine paraffin-embedded sections of ALCL.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01209661
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