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  • 1
    Electronic Resource
    Electronic Resource
    Enhancement of enzymatic degradation of cellulose by application of mixed enzyme systems of different fungal origin (1985)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 5 (1985), S. 27-33 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Individual culture supernatants of six cellulolytic fungi and their mixed enzyme systems were compared in hydrolysis of pure celluloses and cellulose-rich wastes. It has been observed that the hydrolysis rate of pure cellulosie substances in general increased by mixed enzymes as compared to the enzymes supernatant derived from only one microbial source. This effect was particularly high when Trichoderma and Penicillium enzyme systems were operated together.
    Additional Material: 7 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370050108
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Oszillierende-fed-batch-Technik bei der Cellulasegewinnung (1988)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 8 (1988), S. 285-289 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: From different fungal genera some mutant strains were selected which in cellulase formation exhibit reduced catabolite-repression related to several soluble carbon sources. The cellulase production by these mutant strains on the basis of soluble carbon sources is possible if a fed-batch-technique is used for feeding the substrate.The optimum procedure is an oscillating-fed-batch-technique (OFB) with feed-back-controlled intermittent addition of the substrate. The substrate concentration in the medium is adjusted in such a manner to realize oscillations between a limitation for mycelium growth and non-limiting conditions under a substrate concentration which is yet below the repressing concentration for cellulase formation. A suitable feed-back-parameter for the control of substrate addition is the specific CO2-formation of the mycelium.Using OFB the growth in cellulase activity is nearly proportional to the feeded substrate whereas the growth in mycelium is reduced. The productivity of cellulase formation is enhanced in comparison to continuous substrate addition as well as under limiting and non-limiting conditions for growth of mycelium.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370080323
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  • 3
    Electronic Resource
    Electronic Resource
    Spezielle Anwendungsmöglichkeiten mikrobieller ProteasenVortrag gehalten anläßlich des 2. Symposiums der sozialistischen Länder über Biotechnologie, Leipzig 2.-5. 12. 1980 (1981)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 1 (1981), S. 377-386 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: A brief review of the most important properties and fields of application of microbial proteinases are presented. Special possibilities of an industrial utilization of these enzymes are discussed: in the photographic industry for the production and degradation of gelatin as well as for silverless photography, in the food industry for the improvement of the functional properties of proteins as well as for increasing their nutritive value, in organic synthesis for the production of peptides, and in sewage treatment plants for the stabilization of sludge.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370010411
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  • 4
    Electronic Resource
    Electronic Resource
    Rezension (1981)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 1 (1981), S. 391-392 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: No Abstrct.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370010413
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  • 5
    Electronic Resource
    Electronic Resource
    Continuous ethanol production by zymomonas mobilis on an industrial scale (1987)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 7 (1987), S. 307-313 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The specific growth rate of the ethanol producing bacterium Zymomonas mobilis was lower in the presence of oxygen than under anaerobic conditions. Aerobically, considerable amounts of acetaldehyde and acetic acid were formed in addition to the normal fermentation products, ethanol and carbon dioxide. This bacterium contains considerable amounts of pentacyclic triterpenoids, mainly 1, 2, 3, 4-tetrahydroxypentane-29-hopane. It seems that stability and permeability of the cytoplasmic membrane of this rather ethanol tolerant organism is achieved by the hopanoid content. A continuous culture of Zymomonas mobilis produced 60 g/l ethanol over a test period of 39 days. This strain was used for ethanol production from an enzymatically hydrolyzed wheat starch fraction on an industrial scale of 100 m3.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370070405
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  • 6
    Electronic Resource
    Electronic Resource
    Wood and cellulosics. Chichester: Ellis Horwood Limited Publishers, 1987. 664 pp., 69.50 £ (1988)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 8 (1988), S. 283-283 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370080321
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  • 7
    Electronic Resource
    Electronic Resource
    Leipziger biotechnologiesymposium 1986 (1987)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 7 (1987), S. 295-297 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370070402
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  • 8
    Electronic Resource
    Electronic Resource
    Rolle der einzelnen Komponenten des Cellulasekomplexes bei der Hydrolyse unlöslicher Cellulose (1986)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 6 (1986), S. 369-379 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The kinetics of the hydrolysis of microcrystalline cellulose (MC) by a Trichoderma reesei cellulase complex and by the individual endoglucanase (pI 4.4-5.2) and cellobiohydrolase (pI 4.0-4.2) has been studied. A flow chart for the enzymatic hydrolysis of the cellulose has been revealed, which formed a basis for a computer simulation of the kinetic regularities observed. As a result of it, the values of the catalytic rate constants for the individual stages of the enzymatic degradation of MC have been calculated. Then, the synergistic behaviour of endoglucanase and cellobiohydrolase in the hydrolysis of MC has been described both quantitatively and graphically. The relative efficiency of the individual stages for the MC hydrolysis in terms of glucose and cellobiose formation for cellulase complexes of various composition has been calculated. It was quantitatively shown that cellobiohydrolase plays the key role in the MC hydrolysis by T. reesei cellulase preparations, because it gives up to 80% glucose and up to 80-90% cellobiose in the presnce of endoglucanase which in turn plays a relatively minor role in a direct formation of both soluble products of the hydrolysis.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370060412
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  • 9
    Electronic Resource
    Electronic Resource
    A reduced specific ethanol-forming performance of yeast at high biomass concentrations as a result of a changed ethanol-tolerance behaviour of the cells under condition of limitation. II. Proving the effect in high-flow rate fermentations with saccharomyces cerevisiae Sc 5 (1989)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 9 (1989), S. 25-33 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: In growth-factor limited fermentations carried out in continuous high-flow rate fermenters the relationship existing between the specific ethanol formation rate and the ethanol concentration was found to change its characteristics from a linear function into a nonlinear one at high biomass concentrations. The deviation from the linear inhibitory behaviour of the cells was the greater the more the biomass concentration increased.A very good correspondence between the experimentally found productivities of ethanol formation and its adequate values obtained by calculation could be attained by using an improved steady-state productivity model, in which the variability of the function v = f(P) had been considered.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370090105
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  • 10
    Electronic Resource
    Electronic Resource
    Introduction of the tac-promoter by lactose under fermentation conditions (1991)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 11 (1991), S. 23-29 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The use of the expensive IPTG for induction of the tac-promoter is not practicable at the industrial scale because of the large amounts necessary for induction. We developed a system in which IPTG as inducer was replaced by lactose which induces the tac-promoter with the same efficiency. In E. coli CW3011 (pOF1.0) lactose is utilized as inducer and carbon source even in the presence of glucose, presumably as a consequence of the overproduction of lac-permease.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370110107
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