ISSN:
0138-4988
Keywords:
Life Sciences
;
Life Sciences (general)
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Process Engineering, Biotechnology, Nutrition Technology
Notes:
The use of the expensive IPTG for induction of the tac-promoter is not practicable at the industrial scale because of the large amounts necessary for induction. We developed a system in which IPTG as inducer was replaced by lactose which induces the tac-promoter with the same efficiency. In E. coli CW3011 (pOF1.0) lactose is utilized as inducer and carbon source even in the presence of glucose, presumably as a consequence of the overproduction of lac-permease.
Additional Material:
4 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/abio.370110107
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