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  • 1990-1994  (2)
  • Malus x domestica  (1)
  • RFLPs  (1)
  • Ryanodine  (1)
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Material
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  • 1990-1994  (2)
Year
Keywords
  • 1
    Electronic Resource
    Electronic Resource
    Oscillations of receptor-operated cationic current and internal calcium in single guinea-pig ileal smooth muscle cells (1993)
    Springer
    Pflügers Archiv 424 (1993), S. 431-438 
    Details
    ISSN: 1432-2013
    Keywords: Calcium oscillations ; Muscarinic receptor ; Calcium stores ; G protein ; Heparin ; Ryanodine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract In single cells isolated from guinea-pig ileal smooth muscle, held under voltage clamp at −40 mV or −50 mV by patch pipette in the whole-cell recording mode, carbachol (CCh) evoked an oscillatory inward cationic current. The frequency of current oscillations increased with increasing CCh concentration. CCh-evoked current oscillations were followed very closely by oscillations in intracellular free Ca2+ estimated from the Indo-1 signal, and were abolished by inclusion of EGTA in the pipette solution. Ryanodine and heparin, but not nifedipine, blocked the generation of current oscillations. CCh-evoked current oscillations were abolished upon withdrawal of extracellular calcium and restored upon its reintroduction. Inclusion of GTP[γS] in the pipette solution caused the generation of an oscillatory inward current, which was blocked by ryanodine. The present results are consistent with the hypothesis that CChevoked cationic current is gated by activation of a G protein and is steeply dependent on [Ca2+]i, fluctuations in the release of Ca2+ from stores during carbachol's action produce oscillations in [Ca2+]i which cause similar oscillations in the cationic current.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00374905
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Cytoplasmic relatedness of apple landraces and cultivars: a molecular analysis (1992)
    Springer
    Euphytica 66 (1992), S. 99-102 
    Details
    ISSN: 1573-5060
    Keywords: apple ; cytoplasmic grouping ; diversity ; Malus x domestica ; mitochondrial DNA ; RFLPs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Mitochondrial DNA (mtDNA) diversity has been determined in apple landraces and cultivars, based upon restriction fragment length polymorphisms (RFLPs) for several mitochondrial genes. Our analysis includes three cultivars, ‘Golden Delicious’, ‘McIntosh’ and ‘Delicious’, which represent the various patterns of mtDNA polymorphisms previously described (Ishikawa et al., 1992). A total of five RFLPs were detected, allowing classification of the apple genotypes into four cytoplasmic groups: GroupI, ‘Golden Delicious’-type; GroupII, ‘McIntosh’-type; GroupIII, ‘Delicious’-type; and Group IV, ‘Dolgo Crab’-type. European landraces and cultivars were assigned to Groups I, II, and III, while chinese crab apples were placed in either Group III or IV.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023513
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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