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  • 1990-1994  (3)
  • cadaverine  (1)
  • gas and water cresting  (1)
  • pollution  (1)
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Years
  • 1990-1994  (3)
Year
  • 1
    ISSN: 1573-5028
    Keywords: Nicotiana tabacum ; plant transformation ; gene expression ; bacterial lysine decarboxylase ; protein transport ; chloroplasts ; cadaverine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A possible approach for altering alkaloid biosynthesis in plants is the expression of genes encoding key enzymes of a pathway such as lysine decarboxylase (ldc) in transgenic plants. Two strategies were followed here: one focused on expression of the gene in the cytoplasm, the other on subsequent targeting of the protein to the chloroplasts. Theldcgene fromHafnia alvei was therefore (a) placed under the control of the 1′ promoter of the bidirectional Tr promoter fromAgrobacterium tumefaciens Ti- plasmid, and (b) cloned behind therbcS promoter from potato fused to the coding region of therbcS transit peptide. Bothldc constructs, introduced intoNicotiana tabacum with the aid ofA. tumefaciens, were integrated into the plant genome and transcribed as shown by Southern and northern hybridization. However, LDC activity was only detectable in plants expressing mRNA under the control of therbcS promoter directing the LDC fusion protein into chloroplasts with the aid of the transit peptide domain. In plants expressing the processed bacterial enzyme cadaverine levels increased from nearly zero to 0.3–1% of dry mass.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Transport in porous media 15 (1994), S. 251-269 
    ISSN: 1573-1634
    Keywords: Hodograph method ; 2D cresting models ; gas and water cresting
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Technology
    Notes: Abstract Three new applications of the hodograph method to the problem of gas and water cresting towards horizontal wells are presented: (1) A numerical technique, based on the hodograph method, is used to find the shape of the oil/water interface and values of critical heights for the problem of water cresting towards a horizontal well beneath an impermeable plane. (2) By correct implementation of the boundary conditions in the lateral edge drive model, a hodograph solution is found for which the water crest tends to a horizontal asymptote far from the well, rather than tending towards a parabolic curve as in previous approaches. The solution yields integral representations for the lengths of boundary segments and enables an explicit expression for the critical rate to be derived in terms of the distance to the constant potential boundary. (3) The problem of simultaneous gas and water cresting towards a horizontal well in a thin oil column reservoir is solved using the hodograph method, providing shapes of the free interfaces and an expression for the optimal placement of the well with respect to the interfaces.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-3017
    Keywords: biomarkers ; Bluegill ; sediment ; pollution ; EFPC
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract The temporal expression of various biological rsponses was determined in Bluegill SunfishLepomis macrochirus exposed under controlled laboratory conditions to sediment containing high concentrations of polynuclear aromatic hydrocarbons, polychlorinated biphenyls and heavy metals. Liver, gill, blood, kidney, brain, spleen and intestine were removed from Sunfish sampled at 1, 2, 4, 8, 16, and 40 weeks post-exposure. Biomarker data were recorded for specific proteins, enzymatic activities, DNA integrity, and histopathology. Biomarkers in the laboratory exposed fish were similar to those of indigenous Sunfish sampled from the site of origin of the contaminated sediment. Several patterns of development of biomarkers over time were also evident. For example, the responses of certain biomarkers are not time-dependent (i.e., intestine and gill ATPase activities) while that of others, such as brain ATPase activity, liver cytochrome P450 and NADPH content, stress proteins, chromatin proteins and DNA strand breaks, fluctuate over time. Still other biomarkers, such as EROD activity, zinc protoporphyrin content of the blood, and DNA adducts, showed marked increases over time. Such patterns need to be considered when comparing laboratory and field results and deciding which biomarkers to use for biomonitoring programs. Implications for natural selection and population/community level responses are also discussed.
    Type of Medium: Electronic Resource
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