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  • 1990-1994  (4)
  • 1
    Electronic Resource
    Electronic Resource
    Comparison between ELISA and bio tin-la belled probes from cloned cDNA of potato virus X for the detection of virus in crude tuber extracts (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 39 (1990), S. 0 
    Details
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Diseases caused by potato virus X (PVX) are of significant agronomic importance, and early detection is vital. Biotinylated DNA probes were prepared by random-primed labelling from cDNA inserts and used for the detection of PVX in crude extracts of infected tubers. The minimum detection level in these extracts is in the order of femtograms of PVX RNA. Comparison with ELISA showed that the hybridization method is 100–250 times more sensitive. Probes prepared by this method are highly specific for target RNA even in crude tuber extracts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3059.1990.tb02544.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Cloning and nucleotide sequence of the capsid protein and the nuclear inclusion protein (NIb) of potato virus A (1993)
    Springer
    Archives of virology 128 (1993), S. 135-142 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The sequence of the 3′-terminal 2597 nucleotides of potato virus A (PVA) genome has been determined from cDNA clones. An open reading frame was identified and potentially encodes a large polyprotein containing 789 amino acid residues. This large open reading frame was found to have a high similarity to the nuclear inclusion protein (NIb) and the capsid protein (CP) genes of several potyviruses. The data suggest that the PVA NIb and CP are products arising from the maturation of the large polyprotein as observed for other potyviruses. Putative cleavage sites corresponded to consensus sequences NIa/NIb and NIb/CP, respectively, of other potyviruses. The NIb (putative RNA polymerase) and CP are expected to be 516 and 269 amino acid residues (Mr of 58,939 and 30,094), respectively. The non-coding region is 227 nucleotides long, rich in A and U and unlike other viruses. Furthermore, there are two AUG codons in frame in front of the capsid protein gene suggesting an alternative mode for the capsid protein expression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01309794
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    The entire nucleotide sequence and genomic organization of potato aucuba mosaic potexvirus (1994)
    Springer
    Archives of virology 135 (1994), S. 461-469 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The entire nucleotide sequence (7057 nucleotides) of cDNA clones of potato aucuba mosaic virus (PAMV) was determined. The genome contains five major putative open reading frames (ORFs), designated from the 5′ terminus as encoding putative proteins with Mr of 187 379 (187 K), 26 326 (26 K), 12 101 (12 K), 8 183 (8 K) and 27 114 (27 K). The genomic organization of PAMV is essentially the same as that of several other potexviruses. The sizes and composition of the proteins encoded by the ORFs are generally similar to those found in other potexviruses. The genome and the capsid protein gene of PAMV, are the largest among the potexviruses sequenced to date.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01310031
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Biotinylated DNA probes for detecting virus Y and aucuba mosaic virus in leaves and dormant tubers of potato (1992)
    Springer
    Potato research 35 (1992), S. 173-182 
    Details
    ISSN: 1871-4528
    Keywords: non-radioactive probes ; RNA or DNA hybridization ; ELISA ; potato virus Y ; potato aucuba mosaic virus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Detection of potato virus Y (PVY) in dormant potato tubers using the enzyme-linked immunosorbent assay (ELISA) has been reported not to be accurate and reliable and it requires breaking of the dormancy of tubers prior to testing. We describe a simple, practical and highly sensitive hybridization method based on the use of biotinylated DNA probes for detecting PVY and potato aucuba mosaic virus (PAMV) in dilutions made of crude extracts of infected potato leaves and dormant tubers. As little as 50 fg of RNA can be detected by this method, and the probes are highly specific for their targets, even in crude plant extracts. The presence of one virus did not interfere with the detection of the other.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02357611
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    Paper (German National Licenses)
    Fulltext
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