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  • 1
    Electronic Resource
    Electronic Resource
    Comparison between ELISA and bio tin-la belled probes from cloned cDNA of potato virus X for the detection of virus in crude tuber extracts (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 39 (1990), S. 0 
    Details
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Diseases caused by potato virus X (PVX) are of significant agronomic importance, and early detection is vital. Biotinylated DNA probes were prepared by random-primed labelling from cDNA inserts and used for the detection of PVX in crude extracts of infected tubers. The minimum detection level in these extracts is in the order of femtograms of PVX RNA. Comparison with ELISA showed that the hybridization method is 100–250 times more sensitive. Probes prepared by this method are highly specific for target RNA even in crude tuber extracts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3059.1990.tb02544.x
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  • 2
    Electronic Resource
    Electronic Resource
    Cloning and nucleotide sequence of the capsid protein and the nuclear inclusion protein (NIb) of potato virus A (1993)
    Springer
    Archives of virology 128 (1993), S. 135-142 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The sequence of the 3′-terminal 2597 nucleotides of potato virus A (PVA) genome has been determined from cDNA clones. An open reading frame was identified and potentially encodes a large polyprotein containing 789 amino acid residues. This large open reading frame was found to have a high similarity to the nuclear inclusion protein (NIb) and the capsid protein (CP) genes of several potyviruses. The data suggest that the PVA NIb and CP are products arising from the maturation of the large polyprotein as observed for other potyviruses. Putative cleavage sites corresponded to consensus sequences NIa/NIb and NIb/CP, respectively, of other potyviruses. The NIb (putative RNA polymerase) and CP are expected to be 516 and 269 amino acid residues (Mr of 58,939 and 30,094), respectively. The non-coding region is 227 nucleotides long, rich in A and U and unlike other viruses. Furthermore, there are two AUG codons in frame in front of the capsid protein gene suggesting an alternative mode for the capsid protein expression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01309794
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  • 3
    Electronic Resource
    Electronic Resource
    The entire nucleotide sequence and genomic organization of potato aucuba mosaic potexvirus (1994)
    Springer
    Archives of virology 135 (1994), S. 461-469 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The entire nucleotide sequence (7057 nucleotides) of cDNA clones of potato aucuba mosaic virus (PAMV) was determined. The genome contains five major putative open reading frames (ORFs), designated from the 5′ terminus as encoding putative proteins with Mr of 187 379 (187 K), 26 326 (26 K), 12 101 (12 K), 8 183 (8 K) and 27 114 (27 K). The genomic organization of PAMV is essentially the same as that of several other potexviruses. The sizes and composition of the proteins encoded by the ORFs are generally similar to those found in other potexviruses. The genome and the capsid protein gene of PAMV, are the largest among the potexviruses sequenced to date.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01310031
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  • 4
    Electronic Resource
    Electronic Resource
    Identification and in vivo expression of a prokaryotic-like ribosome recognition sequence upstream of the coat protein gene of potato virus X (2000)
    Springer
    Archives of virology 145 (2000), S. 945-956 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary.  Conserved prokaryotic sequence motifs, distinct from the classic Shine-Dalgarno sequence, yet possessing homology to 16S rRNA in E. coli have been identified in a number of plant viruses. In this report, a similar Shine-Dalgarno-like motif located immediately upstream to the CP gene of potato virus X (PVX) was demonstrated to enable expression of a reporter gene in E. coli to approximately one third the level of a similar construct containing the classical Shine-Dalgarno sequence. Both PVX-specific CP and RNA transcripts were detected in chloroplasts purified from transgenic potato plants containing the PVX CP gene and corresponding leader sequence. Protoplasts generated from these transgenic plants were used to demonstrate that expression of the PVX CP from chloroplasts is possible. The implications of these results on the PVX infection cycle are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007050050686
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  • 5
    Electronic Resource
    Electronic Resource
    Immunological detection of the 8K protein of potato virus X (PVX) in cell walls of PVX-infected tobacco and transgenic potato (1997)
    Springer
    Archives of virology 142 (1997), S. 425-433 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary. Open reading frame 4 (ORF4) of the potato virus X (PVX) genome encodes an 8K protein which is a part of the “triple gene block” and is known to play a role in the cell-to-cell movement of the virus in infected plants. To locate the 8K protein and further elucidate the mechanism of cell-to-cell transport of PVX, antibodies were raised against the 8K protein and used to localize this protein in PVX-infected tobacco and in transgenic potato plants expressing the 8K protein both by subcellular fractionation and by immunolabeling with colloidal gold. The results indicated that the 8K protein was localized to the cell wall.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007050050089
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  • 6
    Electronic Resource
    Electronic Resource
    Expression of the PVYO coat protein (CP) under the control of the PVX CP gene leader sequence: protection under greenhouse and field conditions against PVYO and PVYN infection in three potato cultivars (1997)
    Springer
    Theoretical and applied genetics 94 (1997), S. 287-292 
    Details
    ISSN: 1432-2242
    Keywords: Key words Potato virus Y ; Potato virus X ; Transgenic plants ; Translational enhancer ; CPMR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Coat protein-mediated resistance (CPMR), resistance conferred as a result of the expression of viral coat proteins in transgenic plants, has been illustrated to be an effective way of protecting plants against several plant viruses. Nonetheless, consistent protection has not been achieved for transgenic plants expressing the coat protein of potato virus Y (PVY), the type member of the potyvirus family. In this report, three different potato cultivars were transformed with a chimeric construct consisting of the capsid protein (CP) coding sequences of PVY flanked by the AUG codon and the translational enhancer from the coat protein gene of potato virus X (PVX). These cultivars were shown to express high levels of PVY CP and confer a high degree of protection against PVYo and PVYN under both greenhouse and field conditions. In addition, transgenic plants infected with potato virus A (PVA), a related potyvirus, exhibited a delay in virus accumulation, which could be easily overcome with increasing virus concentrations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050412
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  • 7
    Electronic Resource
    Electronic Resource
    Biotinylated DNA probes for detecting virus Y and aucuba mosaic virus in leaves and dormant tubers of potato (1992)
    Springer
    Potato research 35 (1992), S. 173-182 
    Details
    ISSN: 1871-4528
    Keywords: non-radioactive probes ; RNA or DNA hybridization ; ELISA ; potato virus Y ; potato aucuba mosaic virus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Detection of potato virus Y (PVY) in dormant potato tubers using the enzyme-linked immunosorbent assay (ELISA) has been reported not to be accurate and reliable and it requires breaking of the dormancy of tubers prior to testing. We describe a simple, practical and highly sensitive hybridization method based on the use of biotinylated DNA probes for detecting PVY and potato aucuba mosaic virus (PAMV) in dilutions made of crude extracts of infected potato leaves and dormant tubers. As little as 50 fg of RNA can be detected by this method, and the probes are highly specific for their targets, even in crude plant extracts. The presence of one virus did not interfere with the detection of the other.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02357611
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  • 8
    Electronic Resource
    Electronic Resource
    Characterization of the genome of a vaccine strain of canine adenovirus type 1 (1988)
    Springer
    Virus genes 2 (1988), S. 69-81 
    Details
    ISSN: 1572-994X
    Keywords: canine adenovirus type 1 vaccine ; restriction endonuclease analysis ; genomic physical mapping ; deletion mutants ; dog kidney cells ; virulence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Restriction endonuclease cleavage maps have been constructed for the genome of a canine adenovirus type 1 (CAV-1) vaccine strain (CLL; Connaught Laboratories, Ltd., Willowdale, Ontario). Restriction enzyme analyses were also carried out on CAV-1 (CLL) genomes isolated from viral stocks over 8 serial passages in a dog kidney cell line (DK 6722). The right hand 20% of the genome became more heterogeneous in size with increasing passage in DK 6722 cells due to deletions up to 3–4 kb, whereas the left terminal region was stable throughout these passages. A comparative study of CAV-1(CLL) and a virulent strain of CAV-1, Glaxo, revealed that the genome of CAV-1(CLL) was the shorter, by about 480 bp, within the region covering 0.83–0.91 map units. By virtue of its location within the genome and its dispensable nature for viral growth, this region would appear to encompass a genetic sequence corresponding to the E3 region of human adenoviruses. In terms of viral attenuation, the possible importance of the observed differences between CAV-1(CLL) and CAV-1(Glaxo) is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00569737
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