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  • 1990-1994  (2)
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Material
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Year
  • 1
    Electronic Resource
    Electronic Resource
    Localization of the intronless gene coding for calmodulin-like protein CLP to human chromosome 10p13-ter (1993)
    Springer
    Human genetics 〈Berlin〉 90 (1993), S. 496-500 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The functional intronless gene coding for a calmodulin-like protein (CLP) has been localized to human chromosome 10p13-ter. Chromosomal assignment was performed by Southern blot analysis of DNA from human-rodent somatic cell hybrids and amplification of a CLP gene-specific 1090-bp DNA fragment by the polymerase chain reaction (PCR) on DNA from human-hamster cell hybrids. Chromosomal sublocalization was carried out by in situ hybridization of human chromosome metaphase spreads. The CLP gene is the first member of the human calmodulin/calmodulin-like gene family to be chromosomally sublocalized. Its presence near the telomeric end of the short arm of chromosome 10 may be of significance with respect to its highly (epithelial) cell-type restricted expression in vivo and strong downregulation upon malignant transformation. The generation of a human CLP gene-specific sequence tag site specified by the two primers used for PCR should prove useful for future linkage studies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00217447
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The phylogenetic position ofDimastigella trypaniformis within the parasitic kinetoplastids (1994)
    Springer
    Parasitology research 80 (1994), S. 672-679 
    Details
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The nuclear 16S-like rRNA coding regions of two strains of the kinetoplastid flagellateDimastigella trypaniformis Sandon (strain Ulm and strain Glasgow) were sequenced and phylogenetically analyzed. Strain Ulm was isolated from the hindgut contents of the Australian termiteMastotermes darwiniensis Frogatt, whereas strain Glasgow originates from a soil sample in Scotland. After preparation of genomic DNA the 16S-like rRNA coding regions were amplified using polymerase chain reaction (PCR) technology. The amplification products were cloned in a plasmid vector and sequenced according to standard methods. The sequence of the 16S-like rRNA coding region of strain Ulm differs less than 2% from the sequence of strain glasgow, indicating that the two strains are most probably members of one species. Phylogenetic analysis of the sequence data positionedD. trypaniformis Sandon as a deep branching lineage near the root of the kinetoplastid group of flagellates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00932951
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    Paper (German National Licenses)
    Fulltext
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