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  • 1
    Electronic Resource
    Electronic Resource
    Specific sequence amplification of bovine viral diarrhoea virus nucleic acid (1990)
    Springer
    Archives of virology 111 (1990), S. 239-246 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Bovine viral diarrhoea virus (BVDV) causes infection of cattle worldwide and is a common contaminant of cell cultures in the laboratory. Methods of diagnosis for BVDV are time-consuming and inconsistent. We describe the development of an in vitro test based on enzymatic DNA amplification withThermus aquaticus DNA polymerase of sequences of BVDV cDNA reverse transcribed from viral RNA. Specific sequences were amplified from infected cell cultures and clinical material using laboratory and field strains of BVDV including both cytopathic and non-cytopathic isolates. Both plus and minus strands of viral RNA can act as suitable templates for cDNA synthesis prior to sequence amplification. Internal restriction digest of amplified sequences and the co-amplification of multiple sequences increased the specificity of the reaction. The significance of the technique in relation to the diagnosis and understanding of strain differences is also discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01311057
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Effects of doxorubicin on the canine erythroid and myeloid progenitor cells and bone marrow microenvironment (1993)
    Springer
    Comparative clinical pathology 3 (1993), S. 96-101 
    Details
    ISSN: 1433-2981
    Keywords: Bone marrow microenvironment ; Canine ; Doxorubicin ; Erythroid colony-forming unit ; Fibroblast colony-forming unit ; Granulocyte-macrophage colony-forming unit
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The antineoplastic drug doxorubicin is known to cause cytopenias. In humans and in dogs treated with doxorubicin, neutropenia is a common haematological toxicity. To determine the mechanism of doxorubicin haematotoxicity in the dog, in vitro bone marrow progenitor and microenvironment assays were done in the presence of doxorubicin. The 50% inhibitory concentration (IC50) of doxorubicin for canine erythroid colony-forming unit (CFU-E) was 0.042 ± 0.01 μM and for canine granulocyte-macrophage colony-forming unit (CFU-GM) it was 0.0084 ± 0.002 μM To determine the effects of doxorubicin on the bone marrow microenvironment, fibroblast colony-forming unit (CFU-F) assays were performed. The 50% inhibitory concentration for canine CFU-F was 0.030 ± 0.01 μM. Morphologically, the CFU-F were made up of predominantly cells (73 ± 8%) with fibroblast-like morphology. Within the colonies as well as between the colonies, there were cells with macrophage (27 ± 8%) morphology. To support the morphological classification of these cells, cytochemical staining was done. The cells with the fibroblast-like morphology were negative for butyrate esterase and those with macrophage morphology were positive, whereas both cell types were positive for acid phosphatase in the presence or absence of tartrate. Our data show that the effects of doxorubicin on in vitro haematopoiesis in the dog are similar to those described in man.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00368112
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Characterization of oxide structures formed on nickel-chromium alloy during low pressure oxidation at 500–600°C (1990)
    Springer
    Oxidation of metals 33 (1990), S. 457-479 
    Details
    ISSN: 1573-4889
    Keywords: Ni-Cr alloys ; transient oxide structures ; quantitative XPS ; Auger ; TEM ; SEM
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Low pressure oxidation studies of Ni-18%Cr alloy were carried out at temperatures of 500–600°C for very brief periods. Detailed XPS, AES, SEM, and TEM studies identified four stages in the initial oxidation. These are: (1) formation of a mixed nickel-chromium oxide overlayer; (2) growth of submicron-sized oxide nodules; (3) development of dark “hole-like” patches on the surface; and (4) growth of “second generation” oxide nodules. Both types of nodules consist primarily of a nickel structure depleted in oxygen. Their formation appears to result from a very rapid outward movement of nickel from localized defects in the metal. The dark patches result from the presence of a chromium oxide-rich underlayer, which appears to form by a lateral migration of chromium from adjacent oxide/metal interface regions and from grain boundaries.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00666809
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    Paper (German National Licenses)
    Fulltext
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