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  • 1990-1994  (4)
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Material
Years
Year
  • 1
    Electronic Resource
    Electronic Resource
    Immunohistological localization of trypsin in mucus-secreting cell layers of Atlantic salmon, Salmo salar L. (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish diseases 13 (1990), S. 0 
    Details
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Abstract. Immunohistochcmical examination showed that trypsin was present in mucus-secreting cell layers of Atlantic salmon, such as surface epithelial cells of gills and intestine, and epidermal cells of dorsal skin. Trypsin in tissue slices was identified by an immunohisto-chemical technique which used affinity purified immunoglobulins from rabbit antisera against purified salmon pancreatic trypsin as primary antibodies. Most of the positively stained cells appeared to be granulated and secretory. The authors hypothesize that trypsin in mucus-secreting cell layers is a part of the non-specific immune defence of the fish.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2761.1990.tb00778.x
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Partial purification and characterization of extracellular metalloproteases with caseinolytic, aminopeptidolytic and collagenolytic activities from Vibrio anguillarum (1993)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish diseases 16 (1993), S. 0 
    Details
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Abstract. Proteases produced by Vibrio anguillarum were isolated from culture supernatant by ultrafiltration, gel chromatography and ion exchange chromatography. The enzyme(s) were shown to be collagenolytic when assayed with native collagen substrates. In addition, the enzyme(s) hydrolysed azocasein, azocollagen, the collagenase substrate 4-phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-D-Arg and the aminopeptidase substrate L-Leu-pNA effectively. Separation of the proteases by Mono Q ion exchange chromatography and native polyacrylamide gel electrophoresis revealed four distinct protein bands containing caseinase activity. However, only two of the bands showed aminopeptidase activity. The aminopeptidase activities could be separated from the caseinase activities by isoelectric focusing. Secreted proteases of different serotypcs of V. anguillarum showed a heterogeneous caseinolytic pattern. The molecular mass of the major enzyme was estimated at 35kDa as determined by its mobility on SDS-polyacrylamide gels. Serine protease inhibitors like PMSF, TPCK, TLCK and benzamidine had no inhibitory effects on the proteolytic activity when tested with azocasein as substrate. However, the enzyme was strongly inhibited by metal chelators like EDTA and 1, 10-phenanthroline. Also, normal salmon scrum and purified α2-macroglobulin from salmon serum strongly inhibited the caseinolytic activity of the enzyme.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2761.1993.tb00889.x
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Quantification of trypsin with a radioimmunoassay in herring larvae (Clupea harengus) compared with a highly sensitive fluorescence technique to determine tryptic enzyme activity (1992)
    Springer
    Marine biology 113 (1992), S. 469-473 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Enzymatic activity and quantity of the protease trypsin were measured in individual herring larvae (Clupea harengus L.). The enzymatic activity assay was done using a fluorescence technique, and a radioimmunoassay was used for quantification of trypsin. The results are compared and the differences between the techniques discussed. Both methods gave similar results, as high or low values in trypsin quantity were reflected in high or low values of tryptic activity. Quantity and activity were linearly and positively correlated, but small differences between methods were found at the lowest detection limits. Both techniques reflect high variability between individual larvae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00349173
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Effects of a transitory, low food supply in the early life of larval herring (Clupea harengus) on mortality, growth and digestive capacity (1990)
    Springer
    Marine biology 107 (1990), S. 61-66 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In order to assess possible effects of a transitory, low food supply on later development, three groups of Clyde herring larvae (Clupea harengus L.) were exposed in 1989 to different feeding regimes immediately after yolk resorption. Group 1 received a high daily ration of 80 copepods larvae−1 for 31 d, Group 2 a low daily ration of 15 copepods larva−1 for 10 d followed by a high ration (80 copepods larva−1) for 21 d and Group 3 a low ration of 15 to 20 copepods larva−1 for 31 d. After 31 d of feeding, digestive capacity, expressed as the sum of trypsin and trypsinogen, was markedly reduced in Group 2 compared to Group 1, while Group 3 had an even lower digestive capacity. After the switch from low to high ration Group 2 exhibited compensatory growth and caught up with Group 1 both in standard length and content of soluble protein. Group 3 had the lowest growth rates. Mortality was equal in Groups 1 and 2, while Group 3 showed an excess mortality of 40% of the start population. Although Group 2 larvae had caught up with Group 1 in growth at the end of the study, content of trypsin and trypsinogen in Group 2 was only half of that found in Group 1. Thus, comparing effects of a short period of food limitation on future growth, mortality and content of digestive enzymes, the study indicates content of trypsin and trypsinogen to be the most sensitive variable for detection of food limitation in the early stages of exogenous feeding.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01313242
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    Paper (German National Licenses)
    Fulltext
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