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  • 1
    Electronic Resource
    Electronic Resource
    Resonant level lifetime in GaAs-AlAs double-barrier structures including Γ-X mixing (1991)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 69 (1991), S. 8387-8391 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: A quantitative model for calculation of the lifetime of quasibound states, including the Γ-X transfer, in a AlAs-GaAs-AlAs double-barrier structure is presented. When device is designed that a Γ-like energy level approaches to an X-like energy level, anticrossing of the Γ-X transition occurs and the lifetime of the state can be several orders larger than that of a pure Γ system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.348923
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  • 2
    Electronic Resource
    Electronic Resource
    COUPLING BETWEEN 2-D ELECTRONS IN QUANTUM WELLS AND 3-D PHONONS (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 581 (1990), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1990.tb22717.x
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  • 3
    Electronic Resource
    Electronic Resource
    Mycoplasma pneumoniae DNA gyrase genes (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 4 (1990), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: We have identified a clone from a λEMBL3 library containing a 19kb insert of Mycoplasma pneumoniae DNA which includes the genes that encode both subunits of DNA gyrase. The gyrB gene and the 5’end of the gyrA gene have been subcloned into M13. The gryB gene is 1953bp in length and overlaps the gryA gene by a single base. The nucleotide sequence of these subclones has significant homology to previously reported gyrase genes. In terms of the size of gyrB gene and its proximity to the gyrA gene, M. pneumoniae is more similar to Bacillus subtilis than to Escherichia coli.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.1990.tb00687.x
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  • 4
    Electronic Resource
    Electronic Resource
    Identification of gene products of the P1 operon of Mycoplasma pneumoniae (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 5 (1991), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Gene P1 of Mycoplasma pneumoniae, which codes for a major adhesin, is flanked by two sequences with open reading frames designated QRF4 and QRF6 (Inamine et al., 1988b). In order to identify proteins translated from these ORFs, gene fusions between the N-terminus of the RNA replicase of the Escherichia coli bacteriophage MS2 and selected regions of ORF4 and ORF6 were constructed. The corresponding fusion proteins synthesized in Escherichia coli were used to immunize mice. Antisera directed against ORF4-related sequences did not recognize M. pneumoniae antigens in Western blot analysis, but antisera directed against ORF-6-derived fusion proteins reacted with two M. pneumoniae proteins of 40kDa and 90kDa. In addition, some of the antisera also recognized proteins that formed in a sodium dodecyl sulphate/polyacrylamide gel a protein ladder between 115 and 145 kDa.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.1991.tb02110.x
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  • 5
    Electronic Resource
    Electronic Resource
    A physical map of the Mycoplasma genitalium genome (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 4 (1990), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: We report the construction of a physical map of the genome of the human pathogen Mycoplasma genitalium through the use of pulse-field gel electrophoresis. The small size and relative simplicity of this genome permit the arrangement of restriction fragments without having to construct linking clones. The size of the genome has been calculated to be approximately 600 kb and several important genetic determinants have been assigned specific loci on the map.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.1990.tb00638.x
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  • 6
    Electronic Resource
    Electronic Resource
    Cloning of a Microbispora bispora cellobiohydrolase gene in Streptomyces lividans (1993)
    Springer
    Applied microbiology and biotechnology 38 (1993), S. 631-637 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The cellobiohydrolase II (CBHII) of Microbispora bispora, originally cloned in Escherichia coli, was subcloned into Streptomyces lividans using shuttle vectors pSKN 01 and pSKN 02. The enzyme was secreted from Streptomyces, whereas it was intracellular in E. coli. The yields of CBHII produced by S. lividans transformants were 15–20-fold higher than those produced by E. coli transformants. The optimal pH of M. bispora native cellobiohydrolase and the cloned enzyme from S. lividans is 6.5. The thermal and pH stability of CHBII produced in M. bispora, E. coli and S. lividans were compared. Enzyme produced in E. coli was inactivated more rapidly (k = 0.252 min−1 at 90° C; 90% inactivation after 10 min vs. 0.119 min−1 for the others). CBHII was monitored following electrophoretic separation by reaction with a monoclonal antibody. The apparent molecular mass of the protein produced from the S. lividans clone was 93 kDa, the same as that of the native enzyme, but that of the enzyme produced in E. coli was smaller (82 kDa).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00182802
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  • 7
    Electronic Resource
    Electronic Resource
    Copper as an electron trap in GaAs0.6P0.4 (1994)
    Springer
    Applied physics 59 (1994), S. 245-251 
    Details
    ISSN: 1432-0630
    Keywords: 71.00 ; 61.70
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract An electron trap having an energy level of 0.14 eV from the conduction band edge was found in the bulk of copper-diffused VPE-grown n-GaAs0.6P0.4 by conventional DLTS measurements and by pulse-duration dependent capacitance amplitude measurements. The capture cross section at room temperature is about 1.0×10−21 cm2 and has a weak temperature dependence. These properties are attributed to a non-repulsive center having a capturing mechanism which involves multiphonon emission processes with hardly any lattice relaxation. Evolution of the spatial distributions of the traps with time under junction electric field were studied. The results suggest that the trap is positively charged and has a high diffusivity under electric field. The center can thus be identified as positively charged interstitial copper ion rather than some form of copper complexes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00348227
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  • 8
    Electronic Resource
    Electronic Resource
    Tyrosine phosphorylation of vav proto-oncogene product containing SH2 domain and transcription factor motifs (1992)
    [s.l.] : Nature Publishing Group
    Nature 356 (1992), S. 71-74 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] A schematic representation of the primary structure of Vav illustrating the relative position of the putative structural motifs and src homologous domains SH2 and SH3 is illustrated in Fig. la. As many proteins that contain both SH2 and SH3 domains are substrates of tyrosine kinases, we ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/356071a0
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  • 9
    Electronic Resource
    Electronic Resource
    Salla disease variant in a Dutch patient (1992)
    Springer
    European journal of pediatrics 151 (1992), S. 590-595 
    Details
    ISSN: 1432-1076
    Keywords: Sialic acid ; Salla ; Lysosomes ; Polymorphonuclear ; Heterozygote
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A Dutch child with psychomotor retardation, impaired speech, ataxia, sialic acid storage and vacuolized skin fibroblasts and lymphocytes was diagnosed as having free sialic acid storage disease. Slight corneal opacities, pale optic disks at the fundus oculi and vertebral abnormalities, not earlier reported in Salla disease, were peculiar to this case. Free sialic acid was about tenfold increased in urine and cultured fibroblasts, without changes in the glycoconjugate-bound sialic acid pool. A subsequent pregnancy of the patient's mother was monitored by assay of sialic acid in chorionic villi and amniotic fluid. An unaffected foetus was predicted. Sialic acid was also assayed in peripheral blood total leucoyctes, and in mononuclear and polymorphonuclear (PMN) leucocyte subpopulations. Each of these leucocyte fractions from the patient showed 10- to 30-fold increase in sialic acid content. The PMN subpopulation provided the most restricted range of control values and showed slightly increased values for the patient's parents. These results suggest that the assay of sialic acid in PMN might be useful for the identification of heterozygotes in sialic acid storage disease. Studies on a larger number of obligate heterozygotes are needed to confirm this observation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01957729
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  • 10
    Electronic Resource
    Electronic Resource
    Mössbauer effect study of iron decahydrodecaborate chelates containing ferrocenyl group (1990)
    Springer
    Hyperfine interactions 62 (1990), S. 253-253 
    Details
    ISSN: 1572-9540
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02397706
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