Electronic Resource
Oxford, UK
:
Blackwell Publishing Ltd
Journal of neurochemistry
60 (1993), S. 0
ISSN:
1471-4159
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
Abstract: The effect of phloretin on prostaglandin (PG) F2α-induced phosphoinositide hydrolysis and elevation of intracellular Ca2+ concentration was examined in cultured rat astrocytes. Phloretin inhibited PGF2α (1 μM)-induced phosphoinositide hydrolysis in a concentration-dependent manner with an IC50 value of 16 μM. The inhibitory action of phloretin was specific for PGs. The addition of increasing concentrations of phloretin caused progressive shifts of the dose-response curves of PGF2α to the right. In digitoninpermeabilized astrocytes, phloretin (100 μM) inhibited the stimulation induced by PGF2α (1 μM) plus GTPγS (50 μM) without affecting that induced by GTPγS alone. PGF2α at 1 μM transiently increased astrocytic intracellular Ca2+ concentration in 39% of the cells tested. The response was completely blocked by 100 μM phloretin and the calcium response recovered again after washing out phloretin. These results suggest that phloretin is an antagonist of PGF2α receptor linked to phospholipase C in astrocytes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1471-4159.1993.tb03204.x
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