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Notes: Abstract Axillary buds from 5 genotypes of mulberry belonging to 4 species were cultured on modified MS basal medium. A total of 30 media combinations were tried for all the genotypes. The response of axillary buds and the requirement for growth regulators varied with genotype. In Morus indica BAP (0.25–0.5 mg/l), and in M. alba and M. rotondifolia GA3 (0.5–1.0 mg/l)were found to induce sprouting. Two genotypes of M. bombycis, namely Schimanochi and Mizusawa, developed healthy shoots on the incorporation of 2,4-D (0.5–1.0 mg/l) and BAP (0.5–2.0 mg/l), respectively. IBA (0.5 mg/l), along with cytokinin/auxin/gibberellin, had no effect on bud growth but helped root induction. Shoots developed from the axillary buds were further multiplied as nodal explants. MS basal medium supplemented with 0.5 mg/l IBA and LS vitamins was found best to produce healthy plantlets in all the genotypes. An average 89% survival was observed on transferring the plantlets to soil.

Notes: Abstract The degree of excitation of the emulsion target nuclei due to nuclear interactions of oxygen and sulfur projectiles at 200 GeV/nucleon incident energy has been investigated. Using the plausible assumption that the numberN b of slow particles emitted from the struck target nucleus can be interpreted as a measure of the temperatureT of the residual nucleus, we have found that there exists a critical temperatureT c of the excited target nucleus. For Ag and Br target nuclei this temperature corresponds to 〈N b〉≌12 and it is attained when the impact parameters are less than about 4 fm.

Notes: Abstract Intranuclear cascading mechanism one of the important non-linear effects in high energy nucleusnucleus collisions is investigated. The data on multiplicity (n s ) and pseudorapidity (η) distributions of shower particles produced by32S and16O at 200A GeV,16O at 60A GeV,28Si at 14.5A GeV and He at ≈140A GeV are presented and compared with the string model VENUS, which takes into account the cascade interactions of secondary particles. The effect of the intranuclear collisions on the distributions of 〈η〉 versus 〈n s 〉 is discussed for all the beams.

Notes: Abstract The soluble antigens ofEntamoeba histolytica trophozoites were analysed in detail by biochemical and immunochemical methods. The antigen was highly complex and heterogeneous as revcaled by Sephacryl S-300 column chromatography, which showed four distinct fractions. The molecular mass of fractions FI, FII, FIII and FIV was 660, 170, 65 and 13 kDa, respectively. Protein was the major constituent in crude soluble antigen (CSA) and fractions FI and FII (67%, 80% and 90%, respectively). Polysaccharide was predominant in the FIII fraction (59%). Antigenic activity observed after different physico-chemical treatments revealed that CSA and FI antigens were predominantly glycoprotein in nature. However, the antigenicity of FIII antigen was greatly reduced after sodium meta-periodate treatment, whereas no alteration in reactivity was discerned after trypsin treatment. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis demonstrated nearly 28 Coomassie blue bands for CSA and 20, 16, 15 and 3 polypeptide bands for the FI, FII, FIII and FIV fractions, respectively. The molecular mass of the polypeptides of these bands ranged from 210 to 20 kDa. Antigenic activity was observed in CSA and in the first three fractions, both in counter immunoelectrophoresis (CIEP) and in enzyme-linked immunosorbent assay (ELISA). However, the highest antigenic activity was noted in fraction FI. Major immunoreactive polypeptides of CSA and FI antigens against whole trophozoite antibody were observed in the 10- to 170-kDa regions. However, major differences in the immunoreactivity of the two antigens were noted at 116 and 14 kDa for FI antigen and at 84, 30 and 20 kDa for CSA. The binding of the FI antibody to the surface of the live parasite and the loss of immunoreactive polypeptides from the FI antibody after its adsorption with live trophozoites ofE. histolytica suggest a correspondence between FI and surface antigens ofE. histolytica. The efficacy of active immunization with CSA and its different fractions showed that antigenicity and immunogenicity were closely associated with the high-molecular-weight proteins, as 91% protection was observed for the FI antigens, whereas the FII and FIII fractions and CSA provided only 41%, 33% and 41% protection, respectively. These data suggest that FI antigen can be used in the serodiagnosis of and immunoprophylaxis against amoebiasis.

Notes: Abstract Stacks of nuclear emulsion were exposed at the Brookhaven AGS to a 10.6 GeV/n beam of gold nuclei in the BNL 868 experiment. A preliminary analysis of the main features of the interactions of these nuclei is reported here and compared with those observed previously at lower energies,E〈1 GeV/n. The multiplicity and pseudo-rapidity distributions of the singly charged particles emitted both in central and in minimum bias samples of interactions have been analyzed in order to look for any non random effects indicative of the nuclear state during the interactions. The fragmentation characteristics of the gold projectiles appear to be different at 10.6 GeV/n from those at ≦1 GeV/n incident energy. Fission of the gold projectiles, which was relatively frequent at low energy, is almost entirely suppressed. Some of alpha particles from the projectile are emitted with very large transverse momenta. In gold-emulsion interactions the residual target nuclei are significantly smaller than in oxygen and sulfur interactions in emulsion.

Notes: Abstract We have investigated the particle production and fragmentation of nuclei participating in the interactions of 10.6 GeV/n gold nuclei in nuclear emulsions. A new criterion has been found to distinguish between the interactions of these gold nuclei with the light (H,C,N,O) and heavy (Ag, Br) target nuclei in the emulsion. This has allowed separate analyses of the multiplicity and pseudo-rapidity distributions of the singly charged particles emitted in Au-(H,C,N,O) and Au-(Ag,Br) interactions, as well as of the modes of breakup of the projectile and target nuclei. The pseudo-rapidity distributions show strong forward asymmetries, particularly for the interactions with the light nuclei. Heavy target nuclei produce a more severe breakup of the projectile gold nucleus than do the lighter targets. A negative correlation between the number of fragments emitted from the target nuclei and the degree of centrality of the collisions has been observed, which can be attributed to the total destruction of the relatively light target nuclei by these very heavy projectile nuclei.