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  • 1990-1994  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Cloning and light-dependent expression of the gene coding for the P-protein of the glycine decarboxylase complex from peas (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 81 (1991), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The glycine decarboxylase multienzyme complex (EC 2.1.2.10) is located in the mitochondrial matrix and catalyzes a key reaction of the photorespiratory C-2 cycle of plants. The light-dependent control of the synthesis of the P-protein of this complex in pea (Pisum sativum cv. Alaska) leaves was studied using a partial (0.7 kb) cDNA clone. The size of the P-protein mRNA detected by this probe was 3.5 kb. When the mRNA hybrid selected by this probe was translated in vitro, a protein that migrated in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) with an apparent molecular mass of 114 kDa was formed. This protein could be immunoprecipitated with the P-protein-specific antibody. The mass of the mature P-protein on SDS-PAGE is 100 kDa. This suggests the presence of a presequence used to direct the protein to the mitochondrial matrix. Analysis of the concentration of P-protein mRNA showed an approximately 5-fold increase in the abundance of this transcript in light-grown as compared to dark-grown pea seedlings. The time course for the production of P-protein mRNA following exposure of dark-grown peas to white light was compared to that for the H-protein of glycine decarboxylase, the small subunit of ribulose bisphosphate carboxylase/oxygenase, and the chlorophyll a/b binding protein. All 4 mRNAs accumulated with similar kinetics where they remained low during the first 3 h of illumination, increased about 5-fold during the next 8 h, and then maintained a steady state over the next 12 h.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1991.tb05091.x
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  • 2
    Electronic Resource
    Electronic Resource
    A two-dimensional frost-heave model for buried pipelines (1993)
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 36 (1993), S. 2545-2566 
    Details
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: The rigid-ice model of frost heave is one of the most comprehensive frost-heave models but is restricted to one-dimensional cases in its present form. In this paper, the model is extended to two-dimensional problems. The complete formulation of the partial differential equations governing heat, moisture and ice transport in freezing soils is provided. The equations are subsequently solved using the Galerkin finite element method in space and the finite difference method in time. A computer program is developed for the two-dimensional rigid-ice model. A case of freezing around chilled gas pipeline is solved and the numerical results are compared with experimental values, with good agreement between the two sets of results.
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/nme.1620361504
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    Paper (German National Licenses)
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