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  • 1985-1989  (3)
  • 1975-1979
  • 79.70  (2)
  • Kanamycin resistance  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Efficient selection of somatic hybrids in Nicotiana tabacum L. using a combination of drug-resistance markers introduced by transformation (1989)
    Springer
    Theoretical and applied genetics 77 (1989), S. 547-552 
    Details
    ISSN: 1432-2242
    Keywords: Nicotiana tabacum ; Electrofusion ; Selection of somatic hybrid ; Chloramphenicol resistance ; Kanamycin resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Kanamycin resistance and chloramphenicol resistance were introduced separately to two different tobacco plants (Nicotiana tabacum L.) by Agrobacterium-mediated transformation. Leaf mesophyll protoplasts were prepared from the progeny of these transformants and were subjected to electrofusion. On the 10th day and the 20th day after the fusion treatment, respectively, kanamycin (100 mg/l) and chloramphenicol (30 mg/l) were added to the suspension of protoplasts. The parental protoplasts and an unfused mixture of these protoplasts failed to form colonies when this selection procedure was employed. However, three independent fusion experiments yielded more than 7000 doubleresistant colonies with a frequency between 0.30% and 0.54%. All of the surviving colonies showed continuous growth in the presence of the two antibiotics. The majority of regenerated plants resembled, morphologically, a tetraploid tobacco plant, and their somatic chromosome numbers were 2n=96. The leaf segments from these putative somatic hybrids formed calli and proliferated vigorously on a medium that contained both antibiotics. Southern hybridization permitted the detection of the DNA fragments that conferred kanamycin and chloramphenicol resistance on these somatic hybrid plants. The system described here can be considered to be a universal system for the selection of somatic hybrids and is applicable to various combinations of protoplasts in which pre-selected genetic markers are absent.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00274277
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Cluster cations ejected from liquid metal ion source: alkali metals (Li, Na) and group IV elements (Si, Ge, Sn, Pb) (1989)
    Springer
    The European physical journal 12 (1989), S. 225-227 
    Details
    ISSN: 1434-6079
    Keywords: 36.40 ; 79.70 ; 35.20.X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Cluster abundance of Li n + (n≤19), Na n + (n≤25), Si n z+ (n≤8 forz=1, 3≤n≤7 forz=2), Ge n z+ (n≤11 forz=1, 3≤n≤9 forz=2,n=4 forz=3), Sn n z+ (n≤7 forz=1, 3≤n≤9 forz=2,n=4 forz=3) and Pb n z+ (n≤6 forz=1, 5≤n≤7 forz=2) ejected from a liquid metal ion source has been investigated by mass spectrometry. The abundance spectra of alkali metal clusters showed distinct maxima and steps atn=3, 7, 9, 13 and 19 for Li, and atn=3, 5, 11, 13 and 19 for Na. Mass spectra of Si, Ge and Sn clusters were very similar each other, showing intensity drops aftern=4 and 6 (and alson=10 for Ge) for singly charged clusters. The magic numbers observed are discussed in terms of stability of charged clusters.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01426943
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Abundance of Na cluster ions ejected from a liquid metal ion source (1989)
    Springer
    The European physical journal 11 (1989), S. 87-91 
    Details
    ISSN: 1434-6079
    Keywords: 36.40 ; 79.70 ; 35.20.X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Sodium cluster ions Na+ n withn ranging up to 25 have been observed from a liquid sodium ion source by using a magnetic mass analyzer. Ion intensity as a function of cluster size showed distinct steps and local maxima atn=3, 5, 11, 13 and 19 (magic numbers), and a pronounced odd-even alternation. The features in the ion abundance curve are attributed to the relative stability of cluster ions. The observed magic numbers are only partially explained by the electronic shell model, indicating need to include a consideration of atomic structure in a cluster.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01436588
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    Paper (German National Licenses)
    Fulltext
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