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  • 1
    Electronic Resource
    Electronic Resource
    Pinocytosis and locomotion in amoebae (1979)
    Springer
    Protoplasma 100 (1979), S. 33-43 
    Details
    ISSN: 1615-6102
    Keywords: Amoeba proteus ; Ca++-binding sites ; Cytochemical demonstration ; Induced pinocytosis ; Plasma membrane
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Different methods were used to demonstrate the existence of Ca++-binding sites (Ca++-bs) at the plasma membrane ofAmoeba proteus. In pinocytoting animals the number (indicated by the average distanced in nm) and size (average longitudinal axiss in nm) of Ca++-bs at the cytoplasmic surface of the cell membrane were significantly increased (d=162±15;n=41 ands=93±5;n=47) in comparison to controls (d=208 ±21;n=37 ands=59±8;n=45). The ratio of P: Ca obtained by X-ray microanalysis was in the range of 1.5. The differences observed in the two experimental groups of amoebae are explained by conformational changes in the molecular structure and an increased Ca++-permeability of the plasma membrane during induced pinocytosis. Microplasmodia of the acellular slime moldPhysarum polycephalum investigated for comparison were found to have no Ca++-bs at the interior cell surface.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01276299
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    A modified regionally ischemic porcine heart preparation with eligible residual blood flows (1986)
    Springer
    Basic research in cardiology 81 (1986), S. 384-393 
    Details
    ISSN: 1435-1803
    Keywords: infarct size ; pig ; residual blood flow ; ventricular fibrillation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The left anterior descending coronary artery was occluded in each of 28 thoracotomized pigs around an intracoronary catheter for periods between 30 and 240 min followed by 90 min of reperfusion. The catheter was connected via an external pump with another arterial catheter. The pump rate was set to deliver 1.5 ml (group I), 3 ml (group II), or 6 ml blood/min (group III) respectively during ischemia. The distribution of the residual blood flow during ischemia was determined in group II with non-radioactive microspheres. We delineated the risk region by a fluorescent dye and the infarcted tissue with a tetrazolium stain. The higher residual blood flow in groups II and III reduced the incidence of ventricular fibrillation during ischemia from 70% (group I) to 28%, suggesting that the amount of residual blood flow is one important determinant for this rhythm disturbance. The subendocardial-subepicardial blood flow ratio in the risk region of the anterior wall was 41%. Infarcts started to develop after 30 min of ischemia (groups I and II). In all groups necrosis progressed most rapidly within the first 90 min of ischemia indicating that besides the beneficial effect of a high residual blood flow only early reperfusion is able to salvage a substantial amount of jeopardized myocardium. Compared to conventional regionally ischemic canine and porcine heart preparations the described model offers the following advantages: Accurate delineation of the risk region, eligible residual blood flow, reduction of ventricular fibrillation with higher residual blood flows, and the possibility to selectively test the metabolic influence of drugs on ischemic injury while avoiding systemic effects.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01907459
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Intracoronary hyperosmotic mannitol during reperfusion does not affect infarct size in ischemic, reperfused porcine hearts (1985)
    Springer
    Basic research in cardiology 80 (1985), S. 251-259 
    Details
    ISSN: 1435-1803
    Keywords: mannitol ; ischemia ; reperfusion ; infarct size ; pig
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We investigated the effect of reperfusion with hyperosmotic mannitol on the infarct size in porcine hearts. The distal half of the left anterior descending coronary artery was occluded in each of 21 anesthetized pigs for 75 min and was reperfused for 2 h. During reperfusion mannitol (1075 mosmol/kg) was intracoronarily infused at a dose of 0.5 ml/min in 6 pigs (“low” mannitol group), at a dose of 1.5 ml/min in another 6 pigs (“high” mannitol group), and at a dose of 5 ml/min in 3 pigs for the first 8 min of reperfusion (“very high” mannitol group). 6 pigs served as controls. Although mannitol infusion increased plasma osmolality in the ischemic, reperfused myocardium in all experiments, the infarct size expressed as the ratio of the infarcted tissue over the area at risk of necrosis was not significantly influenced. Infarct size amounted to 72±25% in the control group, to 75±14% in the “low” mannitol group, to 78±18% in the “high” mannitol group, and to 93±8% in the “very high” mannitol group. These results clearly indicate that reperfusion with hyperosmotic mannitol after 75 min of ischemia does not exert any beneficial effect on the infarct size.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01907901
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Pinocytosis and locomotion of amoebae: XII. Dynamics and motive force generation during induced pinocytosis in A. proteus (1979)
    Springer
    Cell & tissue research 197 (1979), S. 263-279 
    Details
    ISSN: 1432-0878
    Keywords: Induced pinocytosis ; Dynamics ; Motive force generation ; Light and electron microscopy ; Amoeba proteus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The mechanism of induced pinocytosis was investigated in Amoeba proteus by light and electron microscopy. The application of nine different inducing substances revealed that pinocytotic channel formation, elongation, vesiculation, shortening and disappearance are the result of the successive or simultaneous action of both traction and pressure forces, which are produced by the contractile activity of a plasma membrane-associated layer of filaments ranging from a few hundred nm to several μ in thickness. The initial phase of channel formation is caused by traction forces according to the membrane flow concept, whereas channel elongation and vesiculation mainly result from pressure forces in conjunction with the extrusion of small hyaline pseudopodia. Shortening and disappearance of the pinocytotic channels are brought about by local contractions of the cortical filament layer in the basal region of the hyaline pseudopodia. Experiments using latex beads as marker particles together with inducing substances show that a rapid membrane turnover during pinocytosis can be excluded, and that the plasma membrane slides as an entire structure over the underlying cytoplasm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233919
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    Paper (German National Licenses)
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