ISSN:
1432-2013
Keywords:
Kidney Cortex and Medulla Slices
;
Warburg Technique
;
Fatty Acid Metabolism
;
Oxygen Consumption
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary 1. Palmitate, butyrate and acetate are oxidized by slices of all zones of the rat kidney (cortex, outer and inner medulla). 2. Oxidation rates are the highest in the cortex and the outer medulla, the lowest in the inner medulla. With substrate concentrations of 1 mM palmitate, 4 mM butyrate and 8 mM acetate in Krebs-phosphate buffer (100% O2, 37°C) cortex converts 0.72 μMol palmitate, 2.55 μMol butyrate and 21.5 μMol acetate/g wet wt/90 min into CO2. The corresponding values for the outer medulla are 0.86 μMol palmitate, 1.35 μMol butyrate, 23.3 μMol acetate; for the inner medulla they are 0.15 μMol palmitate, 0.23 μMol butyrate, and 4.2 μMol acetate. 3. At 5% O2/95% N2 the oxidation rate for palmitate was reduced to 9%, for acetate to 14% of the value at 100% O2 in the cortex. In the inner medulla at the low oxygen tension the oxidation rate for palmitate fell only to 22% and that for acetate only to 43%. 4. In a sodium-free incubation medium palmitate and acetate oxidation fell equally to about 50% in all zones. Depression of oxygen consumption under this condition was in the same range. 5. From these results it can be concluded that fatty acids may provide energy for sodium transport in all zones of the kidney, this process beeing however of very different importance in the cortex and the outer medulla, compared with that in the inner medulla.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00587317
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