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  • 1
    Electronic Resource
    Electronic Resource
    Lumbo-peritoneal shunt in non-hydrocephalic patients (1986)
    Springer
    Acta neurochirurgica 80 (1986), S. 90-92 
    Details
    ISSN: 0942-0940
    Keywords: Percutaneous lumbo-peritoneal shunt ; CSF rhinorrhoea ; pseudo tumour cerebri ; CSF shunt ; craniotomy ; syringomyelia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A population of 41 non-hydrocephalic patients in whom a lumboperitoneal shunt (LPS) was inserted for various conditions is reviewed. 19 had persistent cerebro-spinal fluid rhinorrhoea following cranial injury, basal skull surgery or of unknown origin, 3 had recalcitrant benign intra-cranial hypertension, 14 had a persistent bulging craniotomy site after operations for intra-cranial tumours or head trauma, 4 had syringomyelia and 1 had a postoperative cervical meningocele. There was no shunt-related mortality. LPS was effective in treating the initial symptomatology in 31 patients. Further revision or removal of LPS were needed on 9 occasions in 8 patients showing shunt-related mechanical or infectious complications or persistent postural headaches. This report demonstrates the safety of the LPS procedure experienced in another population of 146 patients with communicating hydrocephalus operated on in the meantime. According to the authors' experience, the versatility of the clinical applications of LPS seems well established. LPS should be considered when a CSF diversion is required in patients showing absent or minimal ventricular enlargement in the CT scan.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01812280
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Separation of tonoplast and plasma membrane H+-ATPase from Zucchini hypocotyls by consecutive sucrose and glycerol gradient centrifugation (1985)
    Springer
    Protoplasma 129 (1985), S. 109-119 
    Details
    ISSN: 1615-6102
    Keywords: Cucurbita pepo L. ; Glycerol gradient centrifugation ; H+-ATPase ; Plasma membrane ; Tonoplast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In order to isolate tonoplast and plasma membrane vesicles involved in ATP-dependent proton transport we devised a preparative procedure with two consecutive centrifugations. Three fractions were obtained on a sucrose step gradient: light microsomes, heavy microsomes, and a mitochondria-rich fraction. The light and heavy microsomal fractions were each recentrifuged on an isopycnic glycerol density gradient. Recentrifugation of light microsomes resulted in two fractions with H+-ATPase activity, one equilibrating at a density less than 1.11 g/cm3 and one equilibrating at a density of about 1.17g/cm3. Comparison with marker enzyme activities suggests that the upper fraction was enriched in tonoplast, and the dense fraction with plasma membrane. In addition to marker enzyme content, H+ transport in the H+-ATPase-containing fractions was further characterized with respect to pH dependence, cation and anion dependence, and uncouplers and inhibitors. H+ transport in all fractions was strongly dependent on the presence of halides but no specific stimulation by potassium or any other monovalent cation was found. Of the anions tested, malate and fumarate preferentially stimulated H+ transport in the tonoplast-enriched fraction. It is suggested that a Ca2+/H+ antiporter is present in all fractions. Only H+-ATPase in the plasma membrane-enriched fractions was sensitive to nystatin, an uncoupler, and to orthovanadate, an inhibitor. The tonoplast fraction was more sensitive to nitrate than the plasma membrane-enriched fraction, and all fractions showed some sensitivity to high concentrations of oligomycin. Oligomycin sensitivity was not due to the presence of mitochondria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01279908
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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