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  • 1985-1989  (2)
  • 1870-1879
  • 45L10  (1)
  • Ultrastructure  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Computing 40 (1988), S. 91-109 
    ISSN: 1436-5057
    Keywords: 65R20 ; 45L10 ; Integral equations ; Tikhonov regularization ; parameter selection method
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Description / Table of Contents: Zusammenfassung In dieser Arbeit betrachten wir eine besondere Variante endlich-dimensionaler Tikhonov-Regularisierung schlechtgestellter Operatorgleichungen. Konvergenzraten werden nachgewiesen und eine a-posteriori Parameterwahl wird hergeleitet, die optimale Konvergenzraten bezüglich des Datenfehlers und der endlich-dimensionalen Approximation liefert, ohne Information über die exakte Lösung zu benötigen. Schließlich präsentieren wir auch einige numerische Beispiele, bei denen lineare Splinefunktionen verwendet werden, die die theoretischen Ergebnisse bestätigen.
    Notes: Abstract In this paper we consider a particular variant of finite-dimensional Tikhonov regularization for ill-posed operator equations. Convergence rates are established and an a-posteriori parameter choice method is derived that leads to optimal convergence rates with respect to data errors and with respect to the finite-dimensional subspace, without using any information about the exact solution. Finally, using linear splines we present several numerical examples that confirm the theoretical results.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 128 (1985), S. 184-189 
    ISSN: 1615-6102
    Keywords: Nuclear isolation ; pH ; Protoplasts ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The present communication describes an easy, efficient and rapid method for isolation of nuclei from plant protoplasts. Release of nuclei is accomplished by disruption of protoplasts in an appropriate buffer containing a very low concentration (0.01%) of the detergent Triton X-100. The pH of the nuclei isolation buffer (5.3) played a critical role in the recovery of stable nuclei in large numbers. Supplementation of buffer (10 mM MES) with spermine (0.1 mM), dithiothreitol (2.5 mM), ethylenediaminetetraacetic acid (2.5 mM) and Nad and KCl (10 mM each) improved nuclear yield and quality. With the method developed it is possible to routinely recover 95% nuclei from the protoplasts within 30 minutes. The nuclear preparations are of high purity with little detectable cytoplasmic contamination and no clumping of the nuclei. The structural integrity of the nuclei has been assessed and confirmed by Nomarski differential interference contrast optics and ultrastructural observations.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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