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  • 1985-1989  (4)
  • Acetylcholine  (2)
  • Watershed management  (2)
  • 1
    ISSN: 1432-2013
    Keywords: Salivary gland ; Non-electrolyte permeability ; Reflection coefficient ; Acetylcholine ; Forskolin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Previous studies have suggested that the permeability of exocrine glands to non-electrolytes may change according to the nature and intensity of the stimuli evoking secretion. The purpose of this study was to define the nature of these permeability changes using a method that distinguishes diffusion from solvent drag. Isolated rabbit mandibular salivary glands were perfused with solutions containing14C-labelled non-electrolytes and stimulated with acetylcholine. Diffusive permeability coefficients (P) and solvent-drag filtration coefficients (1-σ) were estimated from the relationship between salivary non-electrolyte concentration and salivary flow rate. Filtration coefficients for urea, ethanediol, glycerol, erythritol and sucrose increased with acetylcholine concentration while, with the exception of urea, the diffusive permeabilities remained virtually unchanged. The effect of increasing acetylcholine concentration can best be explained by postulating an increase in the effective channel radius of the water secretion pathway from 0.40 nm to 0.45 nm together with a small increase in the fraction of the total water flow passing through larger non-selective pores. Forskolin had little effect on either of the permeability parameters except for a small increase in the diffusive permeability to ethanediol.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-2013
    Keywords: Salivary gland ; Intracellular pH ; 31P NMR spectroscopy ; Acetylcholine ; Amiloride ; DIDS ; Na+−H+ exchange
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Intracellular pH (pHi) was measured in the isolated, perfused rabbit mandibular salivary gland by31P NMR spectroscopy. In the unstimulated gland perfused with HCO 3 − /CO2-buffered Ringer's solution, pHi was 7.27±0.01. Continuous stimulation with acetylcholine elicited dose- and time-dependent changes in pHi. 10−6 mol/l acetylcholine caused a brief intracellular acidosis (−0.19±0.06 pH units) followed by an increase in pHi to a more alkaline steady-state value (7.33±0.02). In the absence of perfusate HCO 3 − or in the presence of 10−4 mol/l DIDS (4,4′-diisothiocyanatostilbene-2,2′-disulphonic acid), the transient acidosis was abolished and pHi increased rapidly to give a sustained alkalosis (7.49±0.03 and 7.44±0.03 respectively). In the presence of 10−3 mol/l amiloride, the response to acetylcholine was a rapid decrease in pHi to 7.02±0.02. The data suggest that, during perfusion with HCO 3 − /CO2-buffered solutions, stimulation with acetylcholine results in a transient loss of HCO 3 − from the acinar cells (causing a transient acidosis), and, independently, the activation of Na+−H+ exchange (causing a sustained alkalosis). In the unstimulated gland, DIDS and the HCO 3 − -free perfusate caused decreases in pHi to 7.12±0.02 and 7.04±0.01 respectively. In contrast, amiloride had little effect. The relatively high value of pHi maintained by the unstimulated gland is therefore probably not due to Na+−H+ exchange.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Environmental management 10 (1986), S. 661-670 
    ISSN: 1432-1009
    Keywords: Logging ; Stream biology ; Watershed management ; Aquatic insects
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Clearcutting may alter stream biota by changing light, temperature, nutrients, sediment particle size, or food in the stream. We sampled macroinvertebrates during late summer of 1979 in first and second order headwater streams draining both two- and three-year-old clearcuts and nearby uncut reference areas in northern New England, USA. Periphyton were sampled throughout the summer by placing microscope slides in these streams for 13–37 days. Periphyton cell densities on these slides following incubation were about six times higher in cutover than in reference streams. Green algae (Chlorophyceae) accounted for a higher proportion of total cell numbers in cutover than in reference streams, whereas diatoms (Bacillariophyceae) dominated the reference streams. The macroinvertebrate density in cutover streams was 2–4 times greater than that in the reference streams, but the number of taxa collected was similar in both cutover and reference streams. Higher numbers of mayflies (Ephemeroptera) and/or true flies (Diptera) in the cutover streams accounted for the differences. Because nutrient concentrations in the cutover streams were nearly the same as those in the reference streams, these differences in macroinvertebrate and periphyton densities were apparently caused by higher light levels and temperature in the streams in the clearcuts. Leaving buffer strips along streams will reduce changes in stream biology associated with clearcutting.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-1009
    Keywords: Organic matter ; Watershed management ; Connecticut ; Forest utilization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract The objective of this research was to evaluate the impacts of increasing product removal on biomass and nutrient content of a central hardwood forest ecosystem. Commercial thinning, currently the most common harvesting practice in southern New England, was compared with whole-tree clearcutting or maximum aboveground utilization. Using a paired-watershed approach, we studied three adjacent, first-order streams in Connecticut. During the winter of 1981–82, one was whole-tree clearcut, one was commercially thinned, and one was designated as the untreated reference. Before treatment, living and dead biomass and soil on the whole-tree clearcut site contained 578 Mg ha−1 organic matter, 5 Mg ha−1 nitrogen, 1 Mg ha−1 phosphorus, 5 Mg ha−1 potassium, 4 Mg ha−1 calcium, and 13 Mg ha−1 magnesium. An estimated 158 Mg ha−1 (27% of total organic matter) were removed during the whole-tree harvest. Calcium appeared to be the nutrient most susceptible to depletion with 13% of total site Ca removed in whole-tree clearcut products. In contrast, only 4% (16 Mg ha−1) of the total organic matter and ⩽2% of the total nutrients were removed from the thinned site. Partial cuts appear to be a reliable management option, in general, for minimizing nutrient depletion and maximizing long-term productivity of central hardwood sites. Additional data are needed to evaluate the long-term impacts of more intensive harvests.
    Type of Medium: Electronic Resource
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