Electronic Resource
Springer
Archives of microbiology
147 (1987), S. 68-72
ISSN:
1432-072X
Keywords:
Differential sedimentation
;
Synchronous cultures
;
Bacillus subtilis
;
Mean cell density
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A simple rapid method for preparing synchronous cultures of Bacillus subtilis has been used to investigate changes in density during the cell cycle. Asynchronous cells separated on a stepped Percoll density gradient had a mean cell density of 1.117 g ml-1±0.004. Samples from a synchronous culture exhibited variation (ca. 1.5%) in mean cell density which was greatest at the onset of cell division. An asynchronous control culture showed little variation in density. These results are discussed in relation to previous work on Escherichia coli.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00492907
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