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  • 1985-1989  (2)
  • Cell wall (secondary), deposition  (1)
  • Karyoplast  (1)
  • Cell wall structure
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Material
Years
Year
Keywords
  • 1
    Electronic Resource
    Electronic Resource
    Exocytosis in non-plasmolyzed and plasmolyzed tobacco pollen tubes (1985)
    Springer
    Planta 166 (1985), S. 287-299 
    Details
    ISSN: 1432-2048
    Keywords: Cell wall (secondary), deposition ; Exocytosis ; Nicotiana (pollen tube, exocytosis) ; Plasmolysis ; Pollen tube ; Vesicle fusion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Exocytosis occurring during deposition of secondary wall material was studied by freeze-fracturing ultrarapidly frozen non-plasmolyzed and plasmolyzed tobacco pollen tubes. The secondary wall of tobacco pollen tubes shows a random orientation of microfibrils. This was observed directly on fractures through the tube wall and indirectly as imprints of microfibrils on fracture faces of the plasma membrane of non-plasmolyzed tubes. About half of the plasmatic fracture faces from non-plasmolyzed and plasmolyzed pollen tubes carried hexagonal arrays of intramembraneous particles in between randomly distributed particles. Deposition of secondary wall material was often accompanied by an undulated plasma membrane and the presence of membrane-bound vesicles in invaginations of the plasma membrane, between the plasma membrane and secondary wall and-especially in plasmolyzed tubes-within the secondary wall of tube flanks and wall cap. The findings are discussed in connection with published schemes of membrane behaviour during exocytosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00401164
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Development of subprotoplasts from in vitro-grown tobacco pollen tubes (1988)
    Springer
    Sexual plant reproduction 1 (1988), S. 103-113 
    Details
    ISSN: 1432-2145
    Keywords: Cell chain formation ; Cell wall regeneration ; Cytoplast ; Karyoplast ; Nicotiana (pollen tube subprotoplast) ; Tube formation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Plasmolyzed pollen tubes of Nicotiana tabacum each released one to three subprotoplasts from their tips when treated with wall-degrading enzymes. Wall regeneration and the further development of the subprotoplasts were studied by both light and electron microscopy. Karyoplasts and cytoplasts incubated in a poor culture medium regenerated a cell wall within 60 min; cellulose microfibrils and callose were shown to be present. In 30%–40% of the cells, one-third of which were nucleate, cell chains and tubes developed by polar growth in a ratio of about 1∶1. They sometimes reached a length 7–9 times the diameter of the former subprotoplast within 5 h of incubation. With longer incubation periods the cell wall became two-layered, its ultrastructure resembling that of the pollen tubes. The capacity of cytoplasts to regenerate a wall and develop cell chains and tubes can be explained by the properties ascribed to the cytoplasm of pollen tubes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00189269
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
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