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Effects of bacterial endotoxin on the ciliary activity in the in vitro eustachian tube (1987)

Ohashi, Y. ; Nakai, Y. ; Ikeoka, H. ; [et al.]

Springer

European archives of oto-rhino-laryngology and head & neck 244 (1987), S. 88-90

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ISSN: 1434-4726

Keywords: Ciliary activity ; Otitis media with effusion ; Lipopolysaccharide ; Eustachian tube

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Summary We have used a tissue culture technique and a photoelectric method to examine the direct effect of lipopolysaccharide (LPS) on the ciliary activity present in the eustachian tube. Since LPS possesses the major part of the biological activity of endotoxin, our results show clearly that LPS deteriorates the ciliary activity in a dose-response fashion: LPS does not deteriorate the ciliary activity up to 168 h if its concentration is 1ng/ml or less; 10 ng/ml LPS can cause deterioration of the ciliary activity with extended exposure (more than 96 h); LPS can cause dysfunction of the cilia rather quickly if the concentration is 100 ng/ml or more. Our results show that the ciliary activity in the eustachian tube under clinical conditions can be affected by endotoxin.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00458553

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Surface analysis by sputtered neutral mass spectrometry with electron-beam-excited plasma (1989)

Hashiguchi, Y. ; Hayashi, S. ; Ohtsubo, T. ; [et al.]

Chichester [u.a.] : Wiley-Blackwell

Surface and Interface Analysis 14 (1989), S. 595-597

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ISSN: 0142-2421

Keywords: Chemistry ; Polymer and Materials Science

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Physics

Notes: A new type of sputtered neutral mass spectrometer, named SNART (Sputtered Neutral Analysis - Riken Type), was evaluated. SNART uses an argon plasma that is driven by an electron beam. The plasma is used for both sputtering of the sample and post-ionizing the sputtered neutral atoms. A depth profile of multilayered Ge/Si was taken by SNART to evaluate depth resolution. The quantitativeness of SNART was also checked using a stainless-steel sample. The results were compared with those by conventional SIMS and glow discharge optical emission spectrometry. The following features of SNART were confirmed: (1) high post-ionization efficiency; (2) high depth resolution; (3) high sputtering rate; (4) no matrix effect on post-ionization efficiency; and (5) high quantitativeness.

Additional Material: 5 Ill.