ISSN:
1573-6903
Keywords:
GABA
;
brain slices
;
release
;
GABA aminotransferase
;
GABA aminotransferase
;
aminooxyacetic acid
;
GABA metabolites
;
development
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract The release of [3H]γ-aminobutyric acid (GABA) and its radioactive metabolites from slices of the cerebral cortex, cerebellum, striatum and brain stem of developing and adult mice was studied. The slices were incubated and superfused in the absence and presence of the GABA aminotransferase (GABA-T) inhibitor aminooxyacetic acid (AOAA). Exposure to 100 μM AOAA totally inhibited GABA-T and all radioactivity released from slices was in authentic GABA. In studies on developing brain the 10-μM concentration was also effective enough, except in cerebellar slices. In the absence of AOAA the major part of radioactivity spontaneously released from slices of adult cerebral cortex and cerebellum was tritiated water and still about one third part in the presence of 10 μM AOAA. Potassium stimulation induced only the release of radioactive GABA but not labeled metabolites in both presence and absence of AOAA. AOAA reduced the stimulation-induced release of GABA. It is recommended that the use of GABA-T inhibitors should be discontinued in release experiments. Then labeled GABA must be separated in the effluents from its radioactive breakdown products.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00970763
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