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  • 1
    Electronic Resource
    Electronic Resource
    Microbial accumulation of neptunium (1988)
    Springer
    Journal of industrial microbiology and biotechnology 3 (1988), S. 329-331 
    Details
    ISSN: 1476-5535
    Keywords: Neptunium ; Metal uptake ; Microorganism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Resting cells of several species of microorganisms removed neptunium (Np) from an aqueous solution. Concentrations of up to 15 mg Np per g cells (dry weight) were obtained. Maximum uptake byMicrococcus luteus occurred in less than 10 min.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01569534
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Glutathione pool size affects cell survival after hyperthermic treatment (1985)
    Springer
    Cell biology and toxicology 1 (1985), S. 213-221 
    Details
    ISSN: 1573-6822
    Keywords: cytotoxicity ; glutathione ; hyperthermia ; pool size
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Intracellular glutathione (GSH) concentrations were titrated in Chinese hamster ovary cells by exposure to various concentrations of diethylmaleate (DEM). The various steady state levels of GSH obtained were maintained throughout the experimental time course. Cells were incubated at 42° after DEM addition in order to produce thermal dose response curves using colony formation as the end point. The slope of the dose response curve was subsequently determined and compared to the intracellular GSH concentration. This comparison indicated Chinese hamster ovary cells contain multiple reservoirs of GSH which in turn regulate thermal toxicity in a stepwise manner. Removal of 50% or less of the GSH did not affect thermal sensitivity. A small increase in sensitivity occured when 50 to 80% of the GSH was removed. Removal of greater than 80% of the GSH increased thermal toxicity significantly. The facts that 10 and 20 µM DEM produce extensive GSH depletion and only small changes in survival imply that a threshold concentration of GSH must be removed before thermal toxicity is affected.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00120165
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    Paper (German National Licenses)
    Fulltext
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