Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Kinetics of Protein Phosphorylation in Microvessels Isolated from Rat Brain: Modulation by Second Messengers (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 51 (1988), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The role of second messengers in the regulation of protein phosphorylation was studied in microvessels isolated from rat cerebral cortex. The phosphoproteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and the kinetics of 32P incorporation into specific protein substrates were evaluated by computer-aided x-ray film densitometry. With the use of this method, Ca2+-calmodulin (CAM)-, Ca2+/phospholipid (PK C)-, cyclic GMP (cGMP)-, and cyclic AMP (cAMP)-dependent protein kinases were detected. CAM-dependent protein ki-nase proved to be the major phosphorylating enzyme in the microvascular fraction of the rat cerebral cortex; the activity of cGMP-dependent protein kinase was much higher than that of the cAMP-dependent one. Autophosphoryla-tion of both the α- and β-subunits of CAM-dependent protein kinase and the proteolytic fragment of the PK C enzyme was also detected. The kinetics of phosphorylation of the individual polypeptides indicate the presence in the cerebral endothelium of phosphoprotein phosphatases. The phosphorylation of proteins in the cerebral capillaries was more or less reversible; the addition of second messengers initiated a very rapid increase in 32P incorporation, followed by a slow decrease. Because the intracellular signal transducers like Ca2+ and cyclic nucleotides are frequently regulated by different vasoactive substances in the endothe-lial cells, the modified phosphorylation evoked by these second messengers may be related in vivo to certain changes in the transport processes of the blood-brain barrier.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1988.tb04834.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Effects of Systemic Kainic Acid Administration on Regional Na+, K+-ATPase Activity in Rat Brain (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 49 (1987), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: Changes in the activity of Na+, K+-ATPase and in the water, Na+, and K+ levels in the parietal cortex, hippocampus, and thalamus were investigated in rats 1, 3, 6, and 24 h following systemic kainic acid injection. An increase in Na+, K+-ATPase activity was observed in all three regions 3 h after the treatment, with a subsequent decrease in enzyme activity. The elevation in Na+,K+-ATPase activity was accompanied by an increase in the Na+ content and a decrease in the K+ content. These changes are presumed to occur because of repeated discharges and excessive prolonged depolarization in response to kainic acid. The decreases in Na+,K+-ATPase activity 6 and 24 h following kainic acid treatment coincide with neuropathological damage and edema formation, mainly in the hippocampus and thalamus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1987.tb03397.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Modulation of the Acetylcholine System in the Superior Cervical Ganglion of Rat: Effects of GABA and Hypoglossal Nerve Implantation After In Vivo GABA Treatment (1985)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 44 (1985), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: γ-Aminobutyric acid (GABA) was applied to the superior cervical ganglion (SCG) of CFY rats in vitro and in vivo, with or without implantation of a hypoglossal nerve, to evaluate the effects of these experimental interventions on the acetylcholine (ACh) system, which mainly serves the synaptic transmission of the preganglionic input. Long-lasting GABA microinfusion into the SCG in vivo apparently resulted in a “functional denervation.”This treatment reduced the acetylcholinesterase (AChE; EC 3.1.1.7) activity by 30% (p 〈 0.01) and transiently increased the number of nicotinic acetylcholine receptors, but had no significant effect on the choline acetyltransferase (acetyl-coenzyme A:choline-O-acetyl-transferase; EC 2.3.1.6) activity, the ACh level, or the number of muscarinic acetylcholine receptors. The relative amounts of the different molecular forms of AChE did not change under these conditions. In vivo GABA application to the SCG with a hypoglossal nerve implanted in the presence of intact preganglionic afferent synapses exerted a significant modulatory effect on the AChE activity and its molecular forms. The “hyperin-nervation”of the ganglia led to increases in the AChE activity (to 142.5%, p 〈 0.01) and the 16S molecular form (to 200%, p 〈 0.01). It is concluded that in vivo GABA microinfusion and GABA treatment in the presence of additional cholinergic synapses has a modulatory effect on the elements of the ACh system in the SCG of CFY rats.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1985.tb08771.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    The blood-brain barrier: New aspects to the function of the cerebral endothelium (1986)
    [s.l.] : Nature Publishing Group
    Nature 321 (1986), S. 197-198 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] THE walls of the cerebral microvessels represent a cellular membrane between blood and brain with special physiological, biochemical and morphological properties'. Metabolic coupling of the main functions of cerebral microvessels to the needs of the nerve and glial cells has been described2 and ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/321197a0
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Intraendothelial accumulation of calcium in the hippocampus and thalamus of rats after systemic kainic acid administration (1986)
    Springer
    Acta neuropathologica 72 (1986), S. 111-116 
    Details
    ISSN: 1432-0533
    Keywords: Kamic acid seizures ; Calcium uptake ; Endothelial calcium accumulation ; Hippocampus ; Thalamus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The accumulation of calcium in the hippocampal and thalamic vascular endothelium and the perivascular space was detected histochemically by means of the pyroantimonate technique 30, 60 and 120 min after systemic kainic acid administration. An increased number of calcium pyroantimonate deposits was found in the endothelial mitochondria 60 min after kainate injection. The mitochondria were swollen at this time and vacuoles containing deposits were observed. After 120 min a pronounced perivascular glial swelling was conspicuous, besides the numerouos endothelial mitochondrial deposits. The swollen glial processes contained a large number of pyroantimonate deposits. It seems likely that the transendothelial calcium transport processes are accompanied by intraendothelial calcium accumulation and mitochondrial calcium sequestration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00685971
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Reverse pinocytosis induced in cerebral endothelial cells by injection of histamine into the cerebral ventricle (1988)
    Springer
    Acta neuropathologica 76 (1988), S. 484-488 
    Details
    ISSN: 1432-0533
    Keywords: Histamine ; Cerebral ventricle ; Pinocytosis ; Brain-blood barrier
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Histamine dihydrochloride (10 μg of 500 μg/ml) was infused during 1 min into the lateral cerebral ventricle of rats, which resulted in a significant stimulation of pinocytosis in the endothelial cells. Systemic injections of mepyramine or metiamide could not prevent this activation. In contrast, ranitidine, injected with histamine was able to inhibit the stimulation of pinocytosis. Albumin exudation from the blood was not found. There was also no change in water and electrolyte contents of the brain tissue. The results suggest that histamine reaching the abluminal membrane can activate the pinocytosis in the cerebral endothelial cells in the reverse direction, i.e., from brain to blood, without opening the blood-brain barrier.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00686387
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Letter to the editor (1987)
    Springer
    Acta neurochirurgica 87 (1987), S. 153-154 
    Details
    ISSN: 0942-0940
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01476068
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    Evidence for GABAergic fibers entering the superior cervical ganglion of rat from the preganglionic nerve trunk (1989)
    Springer
    Histochemistry and cell biology 92 (1989), S. 133-136 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The origin of gamma-aminobutyric acid immunoreactive (GABA-IR) nerve fibers present in the superior cervical ganglion (SCG) of rat was investigated. With immunocytochemical techniques many nerve fibers showed GABA-like positivity in the cervical sympathetic trunk, whereas similar staining could not be revealed in the internal carotid nerve or in the external carotid nerve. Ligation of the cervical sympathetic trunk for 24 h resulted a dramatic reduction in the staining density in the ganglion and in the cervical sympathetic trunk distal to the ligature. After transection of the preganglionic nerve fibers for eleven days or more, very few fibers staining for GABA were seen in the ganglion. The immunohistochemical results suggest that a major source of GABA within the SCG is a population of GABAergic axons entering from the preganglionic trunk.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00490232
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 9
    Electronic Resource
    Electronic Resource
    Increased retention of calcium in the dendrites of long-term potentiated CA1 neurons of the hippocampal slice (1988)
    Springer
    Histochemistry and cell biology 90 (1988), S. 185-193 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In view of the importance of calcium in the induction of long-term potentiation (LTP), experiments were carried out to localize calcium at the electron microscopic level in the CA1 region of guinea pig hippocampal slices, following high-frequency stimulation of the Schaffer collaterals. Apart from the ultrastructural localization, a semiquantitative method was used to count the calcium-containing deposits in electron micrographs. Significantly more calcium-containing deposits were seen in the dendrites of the stratum radiatum in slices with LTP than in those without it. A moderate increase of the extradendritic deposits was observed, too. The calcium content of the deposits was determined by means of EGTA incubation and X-ray analysis. The presented results, together with the relevant literature data, underline the importance of calcium-activated processes in postsynaptic structures probably involved in the generation of LTP.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00492506
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 10
    Electronic Resource
    Electronic Resource
    Spatial correlation between sensory regions and the drainage fields of pial veins in rat cerebral cortex (1986)
    Springer
    Experimental brain research 61 (1986), S. 540-548 
    Details
    ISSN: 1432-1106
    Keywords: Cerebral cortex ; Rat ; Evoked potentials ; Cerebral veins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Visual and somatosensory evoked potentials were mapped in the cerebral cortex of adult rats and, after filling the cerebral arteries and veins with dye, the mappings were then compared to the distribution of pial veins. A close relationship was found between the position, size and shape of the occipital venous drainage field and the distribution of visual evoked potentials with high amplitudes and short latencies. Accordingly, such potentials evoked by stimulation of the forepaw and the tailroot were confined to the fronto-parietal drainage field. In the case of individual variations in the expansion and shape of sensory areas, the medial and lateral borders of the occipital drainage field and the medial border of the fronto-parietal drainage field covaried. Only at the common border between these two drainage fields, visual evoked potentials with small amplitudes and long latencies extended into the parietal drainage field and overlapped with somatosensory evoked potentials. This overlapping area corresponds in position to the anterior part of the peristriate cortex. A comparison between the vascular organization and cytoarchitectonic maps of the rat cortex indicates that other parts of the characteristic pattern of venous drainage fields may also correlate with the cytoarchitectonic and functional organization of the cerebral cortex. These observations suggest that during morphogenesis the formation of sensory projections to the cerebral cortex may interact with the angiogenesis, mainly with the development of veins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00237579
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...