ISSN:
1612-1112
Keywords:
Column liquid chromatography
;
Mobile phase volume
;
Ionic solutes
;
Eluent electrolyte
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary A new method for the determination of the mobile phase volume (V m) in liquid chromatography is presented based on the model regarding the retention of ionic solutes in the presence of eluent electrolytes. TheV m value can be determined by measuring the retention volumes of two ions that have the same charge in two eluent electrolyte systems. Compared with the methods using isotopically labelled eluent components or inorganic salts asV m markers, the method presented is proved to give more reasonableV m values for both normal and reversed-phase liquid chromatography. As well as in binary mixed solvent systems, theV m values in single solvent systems can be determined by this method.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02324702
Permalink