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  • 1
    Electronic Resource
    Electronic Resource
    Is motilin a cerebellar peptide in the rat? (1986)
    Springer
    Anatomy and embryology 173 (1986), S. 371-376 
    Details
    ISSN: 1432-0568
    Keywords: Motilin-like immunoreactivity ; RIA ; HPLC ; Cat ; Intestine ; Rat ; Cerebellum ; Purkinje cells ; Dendrites ; Neocortex ; Pyramidal cells ; Hippocampus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Motilin was demonstrated by the immunoperoxidase technique in endocrine cells of the gastrointestinal tract using several specific antisera. Motilin-like immunoreactivity could only be demonstrated with one of these antisera and was observed in Purkinje cells and dendrites of the cerebellum, in pyramidal cells and dendrites of the cerebral cortex and in dendrites of the CA3 field of the hippocampus of the rat. Very low motilin-like immunoreactivity was found in cerebellum as well as in cerebral cortex using radioimmunoassay. However, using reverse phase liquid chromatography combined with UV-detection and radioimmunoassay, no peak of a peptide corresponding to synthetic motilin was detectable in rat cerebellar extracts, in contrast to findings in rat duodenum. The results do not suggest that motilin is an intrinsic neuroactive substance of the cerebellum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00318921
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Somatostatin-like immunoreactivity in non-pyramidal neurons of the human isocortex (1985)
    Springer
    Anatomy and embryology 173 (1985), S. 237-246 
    Details
    ISSN: 1432-0568
    Keywords: Adult human frontal cortex ; Somatostatin ; Immunocytochemistry ; Non-pyramidal neurons ; Interstitial neurons
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The distribution of somatostatin-immunoreactive cell bodies and axons throughout the human isocortex and subjacent white matter was examined. Vibratome sections of cortical tissue (30–40 μm thick) obtained at surgery were treated to reveal the antigen by the unlabelled antibody enzyme method. Two types of somatostatin-immunoreactive axons were present: short, coiled axons and extended ones that follow a straight course in various directions. Somatostatin immunoreactive nerve cell bodies were encountered in layers II–VI and in the subjacent white matter. The majority of labelled cells were found in the white matter and layer VI, and then in layers II and III. The immunoreactive perikarya were fusiform, triangular or multipolar in shape and did not show preferential orientation of their long axis. Frequently, the fusiform neurons in layer VI and in the white matter were aligned parallel to radiate bundles of myelinated fibres. The immunoreactive neurons gave rise to a few thick dendrites. Often thin axon-like processes could also be recognized, originating either from the cell body or from a thicker dendrite. After destaining of the chromogen and counterstaining with aldehydefuchsin and gallocyanin chromealum, the formerly immunoreactive neurons displayed a light and eccentrically located nucleus. The soma contained only a sparse amount of basophilic substance and was nearly devoid of lipofuscin granules. In electron micrographs, the cisterns of the rough endoplasmic reticulum (RER) were localized near the periphery of the soma. Immunoreactivity occurred along membranes of the RER cistern, outer mitochondrial membrane, and in particles 120–150 μm in diameter. Rounded areas (up to a diameter of 1 μm) lacked immunoreactivity. Furthermore, there were a few tiny lysosomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00316304
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Demonstration of atrial natriuretic peptide/cardiodilatin (ANP/CDD)-immunoreactivity in the salt gland of the pekin duck (1989)
    Springer
    Anatomy and embryology 179 (1989), S. 465-469 
    Details
    ISSN: 1432-0568
    Keywords: Atrial natriuretic peptide ; Pekin duck ; Salt gland ; Immunocytochemistry ; High performance liquid chromatography ; Radioimmunoassay
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A novel peptide hormone, atrial natriuretic factor/cardiodilatin (ANP/CDD), was recently isolated and characterized from mammalian heart. Its presence has been demonstrated in several organs that contribute to water and sodium homeostasis, such as salivary glands. This study demonstrates the presence of ANP/CDD immunoreactivity in the salt gland of Pekin ducks by high performance liquid chromatography, radioimmunoassay and immunocytochemistry, using a specific antibody against atriopeptide I. A small number of distinct, ovoid or cuboid shaped ANP/CDD-immunoreactive cells were localized in the connective tissue surrounding and separating the central secretory tubules, whereas no immunostaining was observed in the peripheral tubules. Salt glands of ducks that were adapted to salt water revealed a significant hypertrophy of their secretory lobules. However, no differences were found between the number or localization of immunoreactive cells in the salt gland of salt water-acclimatized ducks and non-stimulated glands of ducks that were housed with ad libitum access to fresh water. Our results indicate that ANP/CDD may play a role in the regulation of sodium secretion in the salt gland of aquatic birds.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00319589
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Immunhistochemischer Nachweis von Oxytocin im Ovar von Säugern (1985)
    Springer
    Archives of gynecology and obstetrics 238 (1985), S. 793-795 
    Details
    ISSN: 1432-0711
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Zusammenfassung Immunhistochemische Befunde zeigen, daß Oxytocin und Neurophysin nicht nur im hypothalamo-neurohypophysären System, sondern auch „ektopisch” im Ovar von Säugern vorkommen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02430219
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  • 5
    Electronic Resource
    Electronic Resource
    Substance P-immunoreactive neurons in the brainstem of the cat related to cardiovascular centers (1985)
    Springer
    Cell & tissue research 241 (1985), S. 31-41 
    Details
    ISSN: 1432-0878
    Keywords: Substance P ; Cardiovascular system ; Central nervous system ; Immunohistochemistry ; Mapping ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The distribution of substance P-immunoreactivity (SP-IR) in the brainstem and spinal cord of normal and colchicine-pretreated cats was analysed using the peroxidase-antiperoxidase (PAP) technique. Numerous SP-IR fibers are present in the nucleus solitarius, nucleus dorsalis nervi vagi and nucleus spinalis nervi trigemini, various parts of the formatio reticularis, substantia grisea centralis mesencephali, locus coeruleus and nucleus parabrachialis. SP-IR perikarya occur in the substantiae gelatinosa and intermedia of the spinal cord, the nucleus spinalis nervi trigemini-pars caudalis, the nucleus dorsalis nervi vagi, and the nucleus solitarius, as well as in the adjacent formatio reticularis and the medullary nuclei of the raphe. In addition, SP-IR cell bodies are located in the nuclei raphe magnus and incertus, ventral and dorsal to the nucleus tegmentalis dorsalis (Gudden), nucleus raphe dorsalis, substantia grisea centralis mensencephali, locus coeruleus, nucleus parabrachialis and colliculus superior. The results indicate that SP-IR neurons may be involved in the regulation of cardiovascular functions both at the central and peripheral level. A peripheral afferent portion seems to terminate in the nucleus solitarius and an efferent part is postulated to originate from the nucleus dorsalis nervi vagi and from the area of the nuclei retroambiguus, ambiguus and retrofacialis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00214623
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    Paper (German National Licenses)
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