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  • 1980-1984  (5)
  • 1975-1979  (5)
  • 1960-1964  (1)
  • 1955-1959  (1)
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  • 1
    Electronic Resource
    Electronic Resource
    Acyclic diastereoselection of the [2,3]-Wittig sigmatropic rearrangement of a series of isomeric crotyl ethers. A conceptual model for the transition-state geometry (1983)
    s.l. : American Chemical Society
    The @journal of organic chemistry 48 (1983), S. 279-281 
    Details
    ISSN: 1520-6904
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/jo00150a033
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  • 2
    Electronic Resource
    Electronic Resource
    Detection of Inositol and Inositol Diphosphate on Paper Chromatograms (1958)
    [s.l.] : Nature Publishing Group
    Nature 181 (1958), S. 1730-1731 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The paper chromatogram is dipped into the first reagent mixture, which is prepared by adding 10 ml. of concentrated nitric acid to 90 ml. of ethanol. It is then drained, and heated in an oven or over a hot plate at 95-100 C. for 10 min. (heating above 105 C. makes the paper yellow). The paper is ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/1811730b0
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  • 3
    Electronic Resource
    Electronic Resource
    Surface antigens on HeLa cells persistently infected with HVJ (Sendai Virus) (1976)
    Springer
    Archives of virology 51 (1976), S. 275-283 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Surface antigens of HeLaHVJ cells, a cell line persistently infected with HVJ, were studied by fluorescent antibody staining. After absorption with concentrated HVJ virions and HeLa cells, anti-HeLaHVJ antiserum was able to demonstrate specific surface fluorescence on HeLaHVJ cells, while this serum no longer reacted with original HeLa cells nor with HVJ virions. During cytolytic infection of HeLa cells with HVJ, this specific surface antigen appeared at an early stage of infection prior to the appearance of newly synthesized HVJ viral antigens and moreover appeared in spite of the inhibition of viral protein synthesis. This antigen was detected neither on HeLa cells infected with other myxoviruses except HVJ nor on various other kinds of cells infected with HVJ. The specific surface antigen was still found on the HeLaHVJ cell surface after incubation at 38° C for two days, while HVJ structural antigens on the cell surface no longer could be detected. Mild short-term treatment of HeLa cells with trypsin, neuraminidase fromvibrio cholerae, phospholipase-C and hyaluronidase failed to expose specific antigen. The antigen was distinguishable from the Forssman and human blood type antigens. The mechanism of appearance of a new antigen on the surface of HeLaHVJ cells remains unclear.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01317931
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Assembly of viral structural proteins in cells infected with a temperature-sensitive mutant derived from an HVJ (Sendai virus) carrier culture (1979)
    Springer
    Archives of virology 61 (1979), S. 163-168 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The processing of virus polypeptides synthesized in cells infected with HVJ (haemagglutinating virus of Japan—the Sendai strain of parainfluenza 1 virus) was studied. Maturation of a temperature-sensitive(ts) mutant (HVJ-pB) derived from an HVJ carrier culture was inhibited at 38° C incubation. A considerable amount of viral components were made at the restrictive temperature. They were, with the exception of the polypeptide HN, well preserved without a great loss of their function and successfully incorporated into virus particles released after lowering the incubation temperature. The membrane (M) protein seems to be essential for virus morphogenesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01320601
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  • 5
    Electronic Resource
    Electronic Resource
    Protection of mice against virulent virus infection by a temperature-sensitive mutant derived from an HVJ (Sendai virus) carrier culture (1979)
    Springer
    Archives of virology 61 (1979), S. 297-304 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Experimental infection with HVJ (haemagglutinating virus of Japan—the Sendai strain of parainfluenza 1 virus) in mice was studied. Aerosol infection of newborn mice with the wild-type virus (HVJ-W) retarded the development of body weight and killed the animals within a few weeks. Large amounts of virus were isolated from both the lungs and the nasal turbinates of infected mice. In contrast, newborn mice exposed by inhalation to a temperature-sensitive(ts) mutant (HVJ-pB) derived from an HVJ carrier culture showed no clinical signs and grew equally well as mock-infected animals. No infectious virus could be recovered from the lungs although thets mutant grew to moderate titre in the nasal turbinates. The prior inoculation of newborn mice with thets mutant virus induced a state of significant resistance to subsequent challenge with the virulent wild-type virus. No replication of challenge virus in both lungs and nasal turbinates could be detected and the animals were protected a lethal infection. It is suggested that an avirulent temperature-sensitive mutant which has lost the capacity to replicate in the lower respiratory tract but is still capable of multiplying in the nasal turbinates may be a promising candidate for use in live vaccines especially against the infectious disease of the lower respiratory tract.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01315016
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  • 6
    Electronic Resource
    Electronic Resource
    Characterization of the polypeptides synthesized in cells infected with a temperature-sensitive mutant derived from an HVJ (Sendai virus) carrier culture (1979)
    Springer
    Archives of virology 61 (1979), S. 23-33 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The intracellular synthesis of virus-specific polypeptides in cells infected with the wild-type virus of HVJ (HVJ-W) (haemagglutinating virus of Japan—the Sendai strain of parainfluenza 1 virus) and with a temperature-sensitive(ts) mutant (HVJ-pB) derived from an HVJ carrier culture has been analysed by polyacrylamide gel electrophoresis. At the permissive temperature (32° C), all of the known virus structural polypeptides were identified in cells infected with each strain of virus and in addition to the non-structural polypeptides B and C, another polypeptide at the region with a molecular weight of 26,000 to 27,000 (26 to 27K) could be detected in infected cells. At the non-permissive temperature (38° C), the synthesis of the polypeptide M was markedly restrained in cells infected with HVJ-pB, while other major virus polypeptides were present in approximately comparable amounts to cells infected with the wild-type virus. A non-structural polypeptide with a molecular weight of 105K was dominant ints mutant infected cells at higher temperatures and disappeared after temperature-shift from 38° to 32° C. The production of the non-structural polypeptides B and 27K was also temperature-sensitive. The molecular weights of the polypeptides B, M and 27K in HVJ-pB infected cells were larger than those of the corresponding polypeptides in HVJ-W infected cells. The synthesis of the M protein in HVJ-pB infected cells started just after lowering the incubation temperature and the newly made M protein was successfully incorporated into virus particles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01320588
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  • 7
    Electronic Resource
    Electronic Resource
    Tryptic peptide analysis of the structural proteins of a temperature-sensitive mutant derived from an HVJ (Sendai virus) carrier culture (1979)
    Springer
    Archives of virology 61 (1979), S. 157-161 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The individual structural polypeptides of HVJ (haemagglutinating virus of Japan—the Sendai strain of parainfluenza 1 virus) were examined by tryptic peptide analysis. [3H]-methionine-labelled structural proteins of the wild-type virus of HVJ (HVJ-W) and [35S]-methionine-labelled corresponding constituent proteins of a temperature-sensitive(ts) mutant (HVJ-pB) derived from an HVJ carrier culture were compared by ion-exchange chromatography on columns of P-type chromobeads. The tryptic peptides of the individual structural proteins showed characteristic elution profiles. In all the structural proteins tested, the chromatographic elution profiles of both strains generally showed a close resemblance. However, certain minor peaks which were present in one strain but absent in the other strain were detected in the preparations of the P, HN, and F polypeptides. Further, analysis of the NP polypeptide showed that a major peak of one strain appeared at a position in the pH gradient different from a seemingly corresponding major peak of the other strain. In the M protein some possibly homologous minor peaks were found to differ between the two strains.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01320600
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  • 8
    Electronic Resource
    Electronic Resource
    Reversible resistance to the antiviral action of interferon observed in altered BHK cells resistant to HVJ (Sendai virus) infection (1984)
    Springer
    Archives of virology 82 (1984), S. 241-246 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Altered baby hamster kidney (BHK-R) cells which were subcultured in the continuous presence of HVJ (hemagglutinating virus of Japan — the Sendai strain of parainfluenza 1 virus) showed a resistance to the antiviral action of both type I and II interferons. No evidence for a direct inactivation of interferon molecules during incubation of BHK-R cells was obtained. After serial subculture of BHK-R cells in growth medium free of HVJ, surface membranes with the proper sialic acid residues were restored and the cells became susceptible to the interferon action. It is suggested that binding sites for interferons might be ranked above HVJ receptors in the “receptor gradient”.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01311167
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  • 9
    Electronic Resource
    Electronic Resource
    Plaque formation by human-origin parainfluenza type 2 virus in established cell lines (1981)
    Springer
    Archives of virology 67 (1981), S. 355-360 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Two strains of parainfluenza type 2 virus formed well-defined plaques in cultures of Vero cells, an established line of African green monkey kidney cells. In the absence of trypsin, satisfactory plaques were formed by the Toshiba strain of virus. When trypsin was added in the overlay medium of Vero cell monolayers, another strain (62-M786) of virus produced plaques. The Toshiba strain was also able to make plaques in HeLa, BHK, and LLC-MK2 (an established line of monkey kidney) cells without trypsin, but not in mouse L cells. The sensitivity of plaque assay was about equal to that of the hemadsorption method.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01314839
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  • 10
    Electronic Resource
    Electronic Resource
    In-beam study of the level crossing resonance on the 4 μs 2 9+ isomer of69Ge in a Zn single crystal (1983)
    Springer
    Hyperfine interactions 14 (1983), S. 315-331 
    Details
    ISSN: 1572-9540
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The time-integral angular distribution was measured as a function of an external magnetic field for γ-rays from the 398 keV 2 9+ state of69Ge populated by bombarding a Zn single crystal with a natural α-beam from a cyclotron. Clear resonances of dispersion as well as Lorentz type having a narrow width consistent with the natural width (τ=4 μs) were observed at Bres=9.15 kG corresponding to the first level-crossing resonance of the 2 9+ state due to the combined collinear magnetic and quadrupole interactions. The effects of noncollinearity were also demonstrated and compared with theory. As an application of this resonance method, the temperature dependence of the quadrupole interaction frequency was measured.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02043304
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