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  • 1
    ISSN: 1432-2048
    Keywords: Asparagine linked oligosaccharides ; Cotyledon ; Glycoprotein ; Lectin ; Phaseolus (lectin structure) ; Phytohemagglutinin (structure)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Phytohemagglutinin, the major lectin in the seeds of the common bean Phaseolus vulgaris L., was isolated by affinity chromatography from cotyledons of nearly mature seeds and from developing cotyledons labeled with [3H]glucosamine, [3H]mannose or [3H]fucose. The protein was subjected to exhaustive proteolysis and the carbohydrate composition of the resulting glycopeptides examined. Two classes of oligosaccharide side-chains were found. The sidechains of the first class are of the high-mannose type, containing two residues of N-acetylglucosamine and 8 or 9 mannose residues. The sidechains of the second class are of the modified type containing N-acetylglucosamine, mannose, fucose, xylose in molar ratios of 2:3.8:0.6:0.5. Two-dimensional gel electrophoresis shows that phytohemagglutinin can be fractionated into seven different glycosylated polypeptides, and that each one contains at least one modified oligosaccharide chain. The results indicate that most glycosylated polypeptides probably contain one chain of each class. The carbohydrate composition of the two types of chains is similar to that found in other plant glycoproteins, but this is the first report of a plant glycoprotein with both highmannose and modified oligosaccharides on the same polypeptide chain.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 71 (1983), S. 313-317 
    ISSN: 1573-5036
    Keywords: Forest ; Glasshouse conditions ; Mycorrhizal fungi ; Nursery ; Seedling response ; Sitka spruce
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Different mycorrhizal fungi were tested for their effectiveness in promoting growth of Sitka spruce seedlings, in two contrasting soils, in a glasshouse pot experiment. In nursery soil,Laccaria amethystina significantly improved growth of seedlings in comparison toL. laccata. Seedlings inoculated with a forest isolate ofThelephora terrestris were significantly larger than those inoculated with a nursery isolate when grown in forest soil. The effectiveness ofComplexipes moniliformis in forest soil was poor in comparison to other mycorrhizal fungi. Strains aswell as species of mycorrhizal fungi affect seedling growth differently. These effects are further influenced by soil type.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 71 (1983), S. 319-323 
    ISSN: 1573-5036
    Keywords: E-strain ; Forest ; Nursery ; Replacement ; Sitka spruce ; Thelephora terrestris
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Occurrence of mycorrhizal types in 4 forest sites and 4 nurseries was determined by mycorrhizal morphology. In total 25 distinct mycorrhizas were found, of which 14 were formed by identifiable fungi. The frequency of occurrence of the ‘E-strain’ fungus, the dominant nursery mycorrhizal fungus, decreases with the age of the outplanted seedling. Some mycorrhizal types were found in all the mature forest sites examined. The fungusT. terrestris was found on all age groups of Sitka spruce studied.
    Type of Medium: Electronic Resource
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