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  • 1980-1984  (8)
  • 1900-1904
  • Histochemistry  (3)
  • Life and Medical Sciences  (3)
  • Laser in neurosurgery  (2)
  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Neurosurgical review 7 (1984), S. 151-158 
    ISSN: 1437-2320
    Schlagwort(e): Laser in neurosurgery ; Nd-YAG laser
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary After many years of experience, in general we prefere the Nd-YAG laser, although the CO2 laser is an advantage in a few specific cases (lipomas, cranial synostosis). While the focused CO2 laser may be used as a cutting instrument in less vascular tissue with little trauma to the surroundings, the Nd-YAG laser produces a homogeneous coagulation with an energy dependent depth effect. Thus, with the Nd-YAG laser residual tumour tissue can be selectively and with a predictable depth effect thermally destroyed. Because of its excellent coagulation property, the use of the Nd-YAG laser is particularly indicated in highly vascular meningeal tumours. The shrinkage of a tumour and its demarcation which is due to the varying absorption properties facilitates the dissection and allows in addition the preservation of normal tissue.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Neurosurgical review 3 (1980), S. 261-266 
    ISSN: 1437-2320
    Schlagwort(e): Nd-YAG laser ; CO2 laser ; Laser in neurosurgery ; Nd-YAG-Laser ; CO2-Laser ; Laser in der Neurochirurgie
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung In den letzten drei Jahren wurden 120 Patienten für einen operativen Eingriff mit dem Laserstrahl ausgesucht, bei denen die Operation aufgrund von Größe, Vaskularisierung oder Lokalisation der Geschwulst technische Schwierigkeiten erwarten ließ. Gegenüber der bipolaren Koagulation glauben wir eine Reihe von Vorteilen gesehen zu haben, das Arbeiten mit dem Laserstrahl erfordert jedoch experimentelle Übung und Erfahrung. Der Schrumpfeffekt der Geschwulst erlaubt von einer kleinen Freilegung aus anatomiegerechtes schonendes Vorgehen ohne wesentlichen Einsatz von Hilfsinstrumenten. Bei starker Vaskularisation bevorzugten wir stets den Neodym-Yag-Laser. Alle Operationen verliefen blutsparend. Mit herkömmlichen Mitteln nicht erreichbare Geschwulstanteile (z. B. Sinuswand, Schädelbasis) können mit dem Laserstrahl zusätzlich ausgeschaltet werden. Der variable Abstand des Laserhandstückes von seinem Zielorgan ermöglicht eine exakte punktförmige sowie eine diffus breitflächige Bestrahlung. Das berührungslose Arbeiten mit dem Laserstrahl bewährt sich besonders am Hirnstamm und Rückenmark, für die mitunter haftenbleibende Pinzettenbranchen zusätzliche Traumatisation bedeuten. Ob der Bestrahlungseffekt bei unseren Fällen zu einer Reduzierung der Rezidivhäufigkeit führt, muß vorerst dahingestellt bleiben, da die Beobachtungszeit noch zu kurz ist. In einer Verkleinerung des Laserhandstückes, d. h. in seiner Umwandlung zu einem vollwertigen Mikroinstrument, sowie in der Kombination Neodym-Yag-Laser (bessere Koagulationsfähigkeit) und CO2-Laser (besserer Schneideeffekt) sehen wir einen weiteren Fortschritt.
    Notizen: Summary Over the last 3 years 120 patients have been operated on using laser techniques. This technique was chosen where technical difficulties were anticipated due to the size, the vascular supply or the localization of the process. Although the use of laser technique requires experimental work, before it can be applied clinically some distinct advantages could be demonstrated when compared with the use of the bipolar cautery. Due to its shrinking effect the laser beam is target oriented and makes the surgical procedure safe without the use of additional instruments. The Nd-YAG laser was preferred in tumors with a rich vascular supply. Thus there was a definitely decreased need for blood transfusions in patients operated on with the laser. Even those parts of the tumors that can not easily be reached, using conventional techniques can now be eliminated by laser irradiation. The variable distance between the handpiece of the laser instrument and the target organ allows a pin point as well as a more diffuse irradiation. Since it is not necessary to touch the tissue, laser techniques are particularly useful in critical areas, e. g. close to the brainstem or the spinal cord, because they make it unnecessary to touch the tissue. The question as to whether the use of laser technique will reduce the rate of recurrent tumor growth needs further studies based on longer follow-up periods. Further technical improvements are needed to make the laser device a true microinstrument. Finally a combination of both laser types — that is the Nd-YAG laser for coagulation and the CO2 laser for cutting -might be a definite advantage.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    ISSN: 1432-2307
    Schlagwort(e): Peanut-Lectin ; Histochemistry ; Mammary cancer ; Human
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Peanut lectin (PNL) is known to bindβ-d-galactosyl-(1-3)-N-acetyl-d-galactosamine, which provides antigenetic determination of the Thomsen-Friedenreich antigen (TFAg). The aim of this study was to analyse the expression of peanut lectin binding sites in mammary carcinomas and to correlate these with tumor type, histological grading, staging and biochemical receptor status. The series comprised 120 invasive mammary carcinomas and 14 cases with normal breast tissue or benign epithelial proliferations as controls. In controls mainly luminal or apical PNL-binding was discovered, however, in all except three carcinomas a cytoplasmatic localisation of TFAg with three major patterns was found: diffuse, granular-globular and vacuolar reactions. The quantitative-qualitative evaluation of the PNL-staining revealed a statistically significant correlation between globular-vacuolar PNL-reaction and tumor type with a higher percentage of this type of reaction in invasive lobular carcinomas as opposed to tubular and invasive ductal carcinomas. Furthermore a statistically significant relationship was disclosed between PNL-histopositivity and estrogen positive - progesterone positive cases. However, the findings of contradictory PNL-status and hormone-receptor status illustrates clearly the difficulty of predicting the biochemical receptor status. No correlation was found between PNL-histochemistry, histological grading, and pathological staging. The practical implications of PNL-histochemistry of mammary carcinomas are discussed.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    Springer
    European archives of oto-rhino-laryngology and head & neck 226 (1980), S. 161-176 
    ISSN: 1434-4726
    Schlagwort(e): Otosklerose ; Histochemie ; Ultrastruktur ; Lysosome ; Oxytalanfaser ; Otosclerosis ; Histochemistry ; Ultrastructure ; Lysosomes ; Oxytalan fibres
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Beschreibung / Inhaltsverzeichnis: Summary In a histochemical and electron microscopical study the pathogenesis of Otosclerosis was investigated. The morphological changes in the extra- and intracellular space indicated a complex metabolic disturbance of all tissue components. The observed chondrocytic chondrolysis stressed the role of the cartilage remnants in the otic capsule as an etiological factor. There was morphological evidence of a superimposed enzymatic defect.
    Notizen: Zusammenfassung In einer histochemischen und ultrastrukturellen Untersuchung werden die pathologischen Bindegewebsveränderungen bei der Otosklerose analysiert. Die Erkrankung führt zu einer komplexen Schädigung aller Bindegewebskomponenten. Es finden sich morphologische Anhalte für einen Enzymdefekt, der dem initialen pathologischen Reiz aufgelagert sein kann. Die beobachtete chondrozytäre Chondrolyse unterstreicht die Bedeutung der Knorpelreste in der otischen Kapsel als pathogenetischen Faktor der Otosklerose.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 5
    Digitale Medien
    Digitale Medien
    Springer
    European archives of oto-rhino-laryngology and head & neck 230 (1981), S. 121-132 
    ISSN: 1434-4726
    Schlagwort(e): Cholesteatom ; Histochemie ; Ultrastruktur ; Lysosomen ; Oxytalanfaser ; Cholesteatoma ; Histochemistry ; Ultrastructure ; Lysosomes ; Oxytalan fibres
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Beschreibung / Inhaltsverzeichnis: Summary In a histochemical and ultrastructural study the pathogenesis of cholesteatoma ostitis is analysed on human tissue. In the subepithelial layer there is evidence of an inflammatory reaction leading to proliferation of granulation tissue with bony invasion. Bony destruction is initiated by osteocytic osteolysis. In the case of cholesteatoma there is a combined action of extraosseous and osseous lysosomal enzymes. The pathological changes of the fibrillar elements suggest that the self-perpetuation of degeneration is maintained by a disturbance of fibrillogenesis. The causal pathogenesis of cholesteatoma formation is discussed as a function of a disturbance between cellular activity, extracellular matrix, and cellular surface.
    Notizen: Zusammenfassung In einer histochemischen und ultrastrukturellen Untersuchung wird die Pathogenese des Cholesteatoms analysiert. Charakteristikum des subepithelialen Gewebes ist die Ausbildung einer entzündlichen Reaktion mit anschließender Formation eines in den Knochen proliferierenden Granulationsgewebes. Der Knochenabbau wird durch osteozytäre Osteolyse eingeleitet. Dadurch kommt es bei der Erkrankung zu einem Zusammenwirken ossär lysosomaler und extraossär lysosomaler Enzyme. Die pathologischen Befunde im Bereich der Faserelemente deuten an, daß der Selbsterhaltungsprozeß der degenerativen Veränderungen durch eine Störung der Fibrillogenese verstärkt wird. Die kausale Genese des Cholesteatoms wird unter Berücksichtigung der gestörten Interaktion zwischen Zellaktivität, Extrazellularmatrix und Zelloberfläche diskutiert.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 6
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Journal of Morphology 180 (1984), S. 105-124 
    ISSN: 0362-2525
    Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: The anatomy of neurons of the stomatogastric nervous system of Ascheta domesticus was studied using heavy metal iontophoresis through cut nerve ends followed by silver intensification. Nineteen categories of neuron are described and compared with neurons known from the stomatogastric nervous system of other insects. Possible functions for the neurons are suggested. Motor neuron candidates are suggested for all parts of the gut served by the stomatogastric nervous system, and axons of sensory neurons of the anterior pharynx are located. There are four neuron types that cannot readily be assigned motor, sensory, or interneuron functions: large dorsal cells of the frontal ganglion; the two neurons of the nervus connectivus, and two categories of neurons in the median neurosecretory cell group of the pars intercerebralis, the axons of which are contained in the stomatogastric nerves.
    Zusätzliches Material: 18 Ill.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 7
    Digitale Medien
    Digitale Medien
    New York, NY : Wiley-Blackwell
    Journal of Morphology 180 (1984), S. 81-103 
    ISSN: 0362-2525
    Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: The distribution of the ganglia and nerves of the stomatogastric nervous system and the innervation of the extrinsic and intrinsic muscles are described. Median unpaired frontal and hypocerebral ganglia and paired ingluvial ganglia are present. The anterior pharynx is innervated by branches of the frontal nerve and by the anterior and posterior pharyngeal nerves, originating from the frontal ganglion. The posterior pharyngeal nerves are linked to nerves innervating the posterior part of the pharynx which have their origin in the hypocerebral ganglion, the anterior portion of which has previously been regarded as part of the recurrent nerve. Paired esophageal nerves run the length of the esophagus and crop between the hypocerebral and and ingluvial ganglia, innervating the muscularis by serial side branches. From each ingluvial ganglion runs an ingluvial nerve which innervates the gizzard and a cecal nerve which innervates the midgut and its ceca. At the posterior end of the midgut there is a poorly developed nerve ring. Nerves running posteriorly from this nerve ring link the stomatogastric nervous system with the proctodeal innervation from the terminal abdominal ganglion.Multipolar peripheral neurons are present on the muscularis of the whole of the foregut, rather randomly distributed on the crop and gizzard but forming fairly definite groupings at some points on the pharynx. Though of varied appearance, these cells could not be divided into discrete morphological categories. Peripheral neurons on the midgut are of different and characteristic morphology, though a few cells of the same appearance as those of the foregut occur at the midgut-hindgut boundary. Nerve fibers on the gut almost invariably terminate on the fibers of the muscularis.
    Zusätzliches Material: 12 Ill.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 8
    Digitale Medien
    Digitale Medien
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 19 (1982), S. 333-347 
    ISSN: 0730-2312
    Schlagwort(e): in vitro transcription ; HSV-1 ; regulation ; RNA polymerase II ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: We used partially purified RNA polymerase II from uninfected (Pol II) and from herpes simplex virus type 1 (HSV-1) infected HEp-2 cells (Pol II-H) to transcribe HSV-1 DNA in vitro. Gel electrophoretic analysis of the products produced from native HSV-1 DNA yielded weight average chain lengths of 4.0 and 3.5 kb for the Pol II and Pol II-H products, respectively. Blot hybridization analyses of the HSV DNA transcripts showed that both enzymes transcribed RNA from essentially all regions of the genome. However, Pol II preferentially transcribed regions coding for the immediate-early or alpha mRNAs, whereas Pol II-H preferentially copied regions coding for the early (β) and late (γ) gene products. Transcriptional analyses of the cloned HSV-1 Bam HI-Q fragment (containing the thymidine kinase (TK) gene) and its subfragments showed that (1) the major transcripts produced by Pol II-H were distinctly different from those produced by Pol II; (2) Pol II and Pol II-H utilized different promoters for the synthesis of major transcripts; (3) both enzymes produced three minor transcripts that were partially overlapping and in opposite direction to the TK gene; and (4) only Pol II-H initiated transcription from the TK promoter. In contrast, both Pol II and Pol II-H generated an identical set of transcripts from an adenovirus 2 early region DNA fragment. The sizes of the products suggest that RNA processing may be occurring in vitro. These results show that HSV-1 infection alters the in vitro transcriptional specificity of RNA polymerase II and demonstrate that this system should be useful for studying in vitro the regulation of gene transcription.
    Zusätzliches Material: 8 Ill.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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