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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 27 (1980), S. 0 
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS A method is described for the axenic mass cultivation of Paramecium tetraurelia strains 51s and 299s. the ciliate is grown in an enriched axenic medium developed by Soldo, Godoy & van Wagtendonk. Under continuous shaking on a rotary shaker, cultures were grown in one-liter Erlenmeyer flasks with 330 ml medium yield cell densities of 32,000 cell/ml and 20,000 cells/ml for strains 299s and 51s respectively. Doubling time is considerably shorter under these conditions than in the conventional static cultures. A 20-liter airlift bioreactor is described in detail which can be used successfully to otain up to 100 g wet weight of Paramecium in a single run; in this reactor the cell density reaches 38,000 cells/ml for strain 299s. and 23,000 cells/ml for 51s. This technic should facilitate the study of minor protein components of the ciliate.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of cutaneous pathology 10 (1983), S. 0 
    ISSN: 1600-0560
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Electron microscopical investigations of untreated solar keratosis, squamous cell carcinoma, and Bowen carcinoma showed intranuclear bundles of intermediate filaments in suprabasal keratinocytes. We found evidence that the filaments are incorporated into the nucleus during atypical mitosis.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 14 (1981), S. 0 
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Normal human peripheral blood B cells that respond to pokeweed mitogen (PWM)-activated irradiated T cells with high-rate immunoglobulin secretion in vitro were analysed with respect to the frequency of the cells stimulated to high-rate immunoglobulin secretion in vitro and whether the progeny of each cell had the potential to secrete one or multiple immunoglobulin isotypes. In vitro cultures containing limiting numbers of human B cells were initiated in the presence of PWM and excess irradiated T cells, and the quantity of IgM, IgG and IgA secreted was determined after 9 days. The level of immunoglobulin secretion per cell in limiting-dilution microcultures was shown to be equivalent to that seen in the routinely used macrocultures, indicating that major loss of B-cell function was not occurring in the microcultures. At limiting B-cell numbers, individual microcultures were often shown to produce immunoglobulin of a single isotype, either IgM, IgG or IgA. Cultures that did produce multiple immunoglobulin isotypes occurred with a frequency predicted by the random distribution of B cells committed to production of a single isotype. A similar independent distribution of IgM anti-tetanus toxoid and IgG anti-tetanus toxoid antibody-producing precursors was observed when B cells selected on the basis of surface IgM were used in the microcultures. These results suggest that PWM-reactive B cells are at a stage of maturation in vivo such that they have the potential to secrete a single immunoglobulin isotype when activated in vitro.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Colloid & polymer science 258 (1980), S. 993-993 
    ISSN: 1435-1536
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    The European physical journal 50 (1983), S. 9-16 
    ISSN: 1434-6036
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The time differential perturbed angular distribution method (TDPAD) has been applied to investigate the electric field gradient produced by radiation induced defects in the cubic lattices of CaF2 and SrF2. In mixed crystals of the alkaline-earth difluorides CaF2 or SrF2 and the rare earth trifluorides PrF3, NdF3 or SmF3 the amplitudesA 22 exp in the TDPAD spectra strongly depend on the rare earth trifluoride concentration. From the fact that the observed amplitudes are well described by a simple statistical model the conclusion can be drawn that the defects produced in CaF2 and SrF2 after proton irradiation are F− interstitials.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    European food research and technology 173 (1981), S. 117-120 
    ISSN: 1438-2385
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Description / Table of Contents: Zusammenfassung Das Problem der Artefaktbildung während der Probenaufarbeitung zur Analyse flüchtiger Nitrosamine wird diskutiert. Extraktionshilfen auf Kieselgur-Basis können unter Einhaltung gewisser Vorsichtsmaßnahmen verwendet werden. Eine detaillierte Analysenvorschrift zur Bestimmung von NDMA in Bier wird beschrieben.
    Notes: Summary The problem of artefact formation during cleanup of samples for the analysis of volatile nitrosamines is discussed. Diatomaceous earth (Kieselgur) extraction aids may be used with certain precautions. A detailed analytical procedure for the analysis of NDMA in beer is given.
    Type of Medium: Electronic Resource
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  • 8
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    Unknown
    Sydney : Periodicals Archive Online (PAO)
    The Australian Journal of Anthropology. 14:3 (1984:Apr.) 165 
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Journal of molecular medicine 62 (1984), S. 826-831 
    ISSN: 1432-1440
    Keywords: Lead content of teeth ; Age-dependent lead levels in permanent teeth ; Continuous low level lead exposure ; Indicator of lead exposure ; Overall lead contamination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Teeth from people aged 5–73 years old, who differ in their occupational type of exposure to lead and who live in different areas of Dortmund, were collected in 1980/81 and were investigated by means of flameless atomic absorption spectrometry. The major result obtained from the 163 permanent and 22 deciduous teeth studied was a statistically significant dependence of the lead content of the permanent teeth on the donor's age. This was superior to all other influences, showing an increase of 3.4% per year of life. There were no sex specific differences, and no correlations with height and body weight were found. To find different lead concentrations within samples of teeth of various living areas and various occupational types of exposure would have required more variation in these variables than was available in the population of our study. It must be emphasized that the reliability of the measured values of dental-lead concentration greatly depends upon the methodical and analytical procedures used, especially because of the overall lead contamination. The analytical method we finally used is described. The factors which influence the dental lead concentration are discussed. The investigation of whole teeth is tolerable. The age-dependent values of the lead content of teeth could be a useful indicator of the overall lead uptake especially in continuous low level lead exposure. Under the conditions described, at least 150 permanent teeth are needed to compare samples of different regions or periods.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Virchows Archiv 389 (1980), S. 253-268 
    ISSN: 1432-2307
    Keywords: Chronic myeloid leukemia ; Chronic megakaryocytic granulocytic myelosis ; Myelofibrosis, beginning ; Histopathology ; Philadelphia chromosome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In 1,083 core biopsies of the bone marrow with myeloproliferative diseases 454 cases or 42% were found to have neoplastic megakaryopoiesis. Neoplasia of megakaryocytes was assumed from the conspicuous cytological atypicality revealed by light microscopy, extending and confirming earlier ultrastructural findings. Histopathology of the bone marrow in these patients was described as chronic megakaryocytic-granulocytic myelosis — CMGM — since neutrophilic granulopoiesis is also apparently neoplastic and both cell lineages showed a complete differentiation to mature forms. CMGM should be separated from the chronic granulocytic leukemia — CGL — which consists of only a single line proliferation. The incidence of CGL in our total of 1,083 patients was 25%. Both entities are included in chronic myeloid leukemia — CML — because of the demonstration of the Philadelphia chromosome in the hematopoietic cells of these two groups of patients. Primary or idiopathic thrombocythemia has to be differentiated from CMGM since there is no evidence for malignancy of the granulocytic series.
    Type of Medium: Electronic Resource
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