Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    facet.materialart.
    Unknown
    An environment for sustainable research software in Germany and beyond: current state, open challenges, and call for action [version 1; peer review: 1 approved, 1 approved with reservations] (2020)
    Details
    Publication Date: 2020-12-15
    Description: Research software has become a central asset in academic research. It optimizes existing and enables new research methods, implements and embeds research knowledge, and constitutes an essential research product in itself. Research software must be sustainable in order to understand, replicate, reproduce, and build upon existing research or conduct new research effectively. In other words, software must be available, discoverable, usable, and adaptable to new needs, both now and in the future. Research software therefore requires an environment that supports sustainability. Hence, a change is needed in the way research software development and maintenance are currently motivated, incentivized, funded, structurally and infrastructurally supported, and legally treated. Failing to do so will threaten the quality and validity of research. In this paper, we identify challenges for research software sustainability in Germany and beyond, in terms of motivation, selection, research software engineering personnel, funding, infrastructure, and legal aspects. Besides researchers, we specifically address political and academic decision-makers to increase awareness of the importance and needs of sustainable research software practices. In particular, we recommend strategies and measures to create an environment for sustainable research software, with the ultimate goal to ensure that software-driven research is valid, reproducible and sustainable, and that software is recognized as a first class citizen in research. This paper is the outcome of two workshops run in Germany in 2019, at deRSE19 - the first International Conference of Research Software Engineers in Germany - and a dedicated DFG-supported follow-up workshop in Berlin.
    Language: English
    Type: article , doc-type:article
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    OPUS
  • 2
    Electronic Resource
    Electronic Resource
    Distribution of macrophages during fish development: an immunohistochemical study in carp (Cyprinus carpio, L.) (1998)
    Springer
    Anatomy and embryology 198 (1998), S. 31-41 
    Details
    ISSN: 1432-0568
    Keywords: Key words Monoclonal antibody ; Monocytes/macrophages ; Fish ; Ontogeny
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  A monoclonal antibody against carp macrophages (WCL15) has been utilised in flow cytometry, immuno-histochemistry and immuno-electron microscopy to assess the distribution of monocytes/macrophages in developing carp lymphoid tissues. In suspensions of living cells WCL15 reacted strongly with cytoplasm and plasmic membrane of macrophages. It also cross-reacted with a subpopulation of thrombocytes, but this reaction could be neglected by double immunostaining in combination with a thrombocyte-specific marker. In Bouin-fixed tissues the antibody distinctly recognised macrophages. Macrophages were found from day 2 post-fertilisation in head kidney and in the dorsal portion of the yolk sac epithelium. From 1 week onwards macrophages were found scattered in thymus and gut and during the second week in spleen. Macrophages increased in number in all lymphoid tissues until the 6–8th week post-fertilisation, but they decreased except in thymus, where they became localised mainly in the cortical-medullary boundary, and in white pulp areas of head kidney. The role of macrophages in allowing an early non-specific defence in young fish and in co-operating during the differentiation processes of T-cells and B-cells is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004290050162
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Acute hyperparathyroidism (1980)
    Springer
    Journal of molecular medicine 58 (1980), S. 501-510 
    Details
    ISSN: 1432-1440
    Keywords: Primärer Hyperparathyreoidismus, klinische und klinisch-chemische Befunde ; Akuter Hyperparathyreoidismus ; Ultrastruktur ; Hypercalciämie ; Parathormonspiegel ; Primary hyperparathyroidism, clinical and laboratory findings ; Acute hyperparathyroidism ; Ultrastructure ; Hypercalcemia ; Parathormone, blood level
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary In a series of 6 patients out of 47 cases with extrarenal hyperparathyroidism we investigated the clinical, laboratory and ultrastructural peculiarities of acute hyperparathyroidism. It was found that there are characteristic differences between both entities which, however, are fluid. Clinically more severe were neuromuscular psychiatric and mental signs. In the laboratory parameters PTH determined by radio-immunoassay and calcium were higher whereas hemoglobin was lower. The weights of the adenomas did not differ in both groups and this was also true for light microscopy findings. Electron microscopy revealed accelerated hormone extrusion and autodigestion of retrieved membrane material in the cases of acute hyperparathyroidism similar to characteristics of parathyroid cells stimulated by hypocalcemia in tissue culture.
    Notes: Zusammenfassung Bei 6 Kranken mit akutem Hyperparathyreoidismus werden klinische Befunde, Labordaten und Besonderheiten in der Ultrastruktur der Nebenschilddrüsen den Daten von 47 Kranken mit extrarenalen Hyperparathyreoidismus gegenübergestellt. Typische Unterschiede konnten aufgezeigt werden, die Übergänge sind aber fließend. Beim klinischen Verlauf waren neuromuskuläre Zeichen, psychiatrische und Störungen des Bewußtseins deutlicher in der ersten Gruppe. Beim Vergleich der Labordaten war radioimmunchemisches Parathormon höher in der akuten Gruppe und das Hämoglobin niedriger. Das Gewicht der Adenome und die lichtmikroskopischen Befunde waren nicht unterschiedlich in beiden Gruppen. Die elektronenmikroskopischen Studien des Adenomgewebes zeigten eine erhöhte Hormonausschüttung mit einer deutlichen Beschleunigung der Autodigestion von Membranmaterial. Diese Veränderungen entsprechen etwa denen, wie sie an Nebenschilddrüsengewebe in der Gewebekultur nach Stimulation durch Hypocalciämie gefunden werden.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01477067
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Der akute Hyperparathyreoidismus bei primärer Wasserheller-Zellen-Hyperplasie (1974)
    Springer
    Journal of molecular medicine 52 (1974), S. 1063-1069 
    Details
    ISSN: 1432-1440
    Keywords: Parathyroid gland ; primary water-clear hyperplasia ; acute hyperparathyroidism ; clinical findings ; electron microscopy ; Nebenschilddrüse ; primäre wasserhelle Zellenhyperplasie ; akuter Hyperparathyreoidismus ; klinische Symptomatik ; Elektronenmikroskopie
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Klinische und morphologische Befunde wurden bei einer Patientin mit akutem Hyperparathyreoidismus bei primaärer wasserheller Zellenhyperplasie vorgestellt und miteinander verglichen. Von klinischer Sicht aus ist insbesondere die lange bestehende Anamnese, der Befund eines primären Hyperparathyreoidismus hoher Aktivität und das plötzliche Auftreten der letal endenden Krise bemerkenswert. Die klinische Symptomatik sowie die Differentialdiagnose dieser seltenen, jedoch lebensbedrohlichen Komplikation wird eingehend erörtert und zusammen mit den wenigen Literaturangaben dazu besprochen. Die elektronenmikroskopischen Untersuchungen an den wasserhellen Zellen kann fließende übergänge zwischen Hauptzellen-ähnlichen Zellgruppen und typischen Zellen mit weitgehender vakuoliger Degeneration des Cytoplasmas aufzeigen, ebenso wie vakuolig entartete oxyphile Zellen. Die Hauptzellen-ähnlichen, organellenreichen Formen werden als eigentlichen Parathormon produzierende Zellen angesehen und die nur sehr wenigen vorhandenen Sekretgranula auf die plötzliche Ausschüttung in der hyperparathyreoten Krise zurückgeführt. In den Vakuolen werden kristallgitterartige Strukturen neben amorphen Anteilen und Lipide gesehen. Folglich könnte die primäre wasserhelle Zellenhyperplasie als Sonderform der primären Hauptzellenhyperplasie angesprochen werden, bei der ein möglicher Synthesedefekt wohl im Bereiche des Golgiapparates vorliegt, der das Cytoplasma von Hauptzellen und oxyphilen Zellen mit vakuolig entarteten Sekretprodukten anfüllt.
    Notes: Summary Clinical and morphological findings are presented in a patient with acute hyperparathyroidism in primary water-clear cell hyperplasia and compared with each other. The long lasting clinical history, the symptoms of primary hyperparathyroidism of high activity, and the very sudden onset of a crisis with a lethal outcome were thought to be remarkable. Discussion of the symptoms and differential diagnosis of this rarely occuring but fatal complication is followed by a review of the pertinent literature. Electron microscopy shows the water-clear cells to exhibit transitional forms between chief cells and typical completely vacuolated cells besides oxyphils with vacuoles. The more chief cell like form is considered to display the really parathormone producing cell. An only very small amount of secretory granules is in consequence to the sudden exocytosis in hyperparathyroid crisis. The vacuoles contain crystal lattice structures, amorphous material and lipids. The primary water-clear cell hyperplasia could be a special form of the primary chief cell hyperplasia with a certain defect in the Golgi apparatus leading to a continuous filling of the cytoplasma of chief cells and oxyphil cells by vacuolated secretory products.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01468993
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Über Unterschiede in der cellulären Feinstruktur von Nebenschilddrüsen bei schweren und leichten Formen des sekundären Hyperparathyreoidismus (1974)
    Springer
    Journal of molecular medicine 52 (1974), S. 444-450 
    Details
    ISSN: 1432-1440
    Keywords: Parathyroid gland ; secondary hyperparathyroidism ; activated chief cell ; secretory cycle ; electron microscopy ; Nebenschilddrüse ; sekundärer Hyperparathyreoidismus ; stimulierte Hauptzelle ; sekretorischer Cyclus ; Elektronenmikroskopie
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Bei zwei klinisch stark unterschiedlich ausgeprägten Formen des sekundären, renal bedingten Hyperparathyreoidismus (Hyperpara) wird die Feinstruktur der hyperplastischen Epithelkörper (EK) miteinander verglichen. Die Beobachtungen lassen dabei ein cyclisches Geschehen im Sekretionsablauf der stimulierten Hauptzelle erkennen, wie es bisher nur bei Tieren beobachtet wurde. Bei dem Patienten mit dem endokrin hochaktiven Hyperpara ist einmal die rein numerische Vermehrung der EK-Zellen (Gesamtgewicht der EK) entscheidend für die Ausprägung des klinischen Krankheitsbildes, zum anderen ist aber auch eine Änderung in der Funktionswertigkeit eingetreten, in dem Sinne, daß sich weitaus mehr Zellgruppen auf dem Höhepunkt der Sekretionsphase des funktionellen Cyclus befinden.
    Notes: Summary The fine structure of parathyroid gland hyperplasia is compared in two cases of secondary (renal) hyperparathyroidism of extremely different clinical course. The results suggest the presence of a secretory cycle in the activated chief cell until now only seen in animals. In the patient with the hyperparathyroidism of high endocrine activity two facts are decisive for the severe clinical course: first the increase in absolute number of parathyroid cells (the total weight of the parathyroid glands), second an alteration of the functional state that means, an increased number of cell groups according to their fine structure are at the peak of the secretory phase of their functional cycle.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01468586
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Effect of temperature on membrane fluidity and calcium conductance of the excitable ciliary membrane from Paramecium
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Biomembranes 769 (1984), S. 253-260 
    Details
    ISSN: 0005-2736
    Keywords: (Paramecium) ; Ca^2^+ transport ; Ciliary membrane ; Fluorescence anisotropy ; Membrane fluidity ; Temperature dependence
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0005-2736(84)90030-0
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Incorporation of [^1^8O]water into 4-hydroxybenzoic acid in the reaction of 4-chlorobenzoate dehalogenase from Pseudomonas spec. CBS 3
    Amsterdam : Elsevier
    Biochemical and Biophysical Research Communications 124 (1984), S. 178-182 
    Details
    ISSN: 0006-291X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0006-291X(84)90933-1
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    Characterization of carp thrombocytes with specific monoclonal antibodies (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 49 (1996), S. 0 
    Details
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Monoclonal antibodies (WCL6) specific for carp Cyprinus carpio thrombocytes were produced by immunizing mice with membrane lysates of IgM-negative peripheral blood leucocytes (PBL) and selected on the negative reaction with B cells. WCL6 was reactive with a membrane molecule of approximately 90 kDa and to a lesser extent with molecules of approximately 95 and 110 kDa. In general, between 30 and 40% of PBL were WCL6+ and appeared to be round to spindle-shaped cells. Immunohistochemical analysis of cryo-sections showed much higher numbers of WCL6+ cells in the spleen than in the pronephros, intestine and thymus. Flow cytometric analysis of cell suspensions isolated from these organs only revealed WCL6+ cells (4–10%) in the spleen. Electron microscopy of immunogold-stained WCL6+ PBL showed round but also some spindle-shaped cells with canalicular and granular structures, and a more irregular and electron-dense nucleus than found in lymphocytes. WCL6+ cells with electrondense pyknotic nuclei (without a clear nuclear envelope) were found also and their frequency increased with the length of the isolation and staining procedure used. In the spleen, several differentiation steps of WCL6+ cells were found and hence the spleen seems to be the thrombopoietic organ in carp. Thrombocytes from blood could be activated with collagen; the collagen-activated cells showed a higher side (90°) scatter by flow cytometric analysis and finally considerable cell death.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1095-8649.1996.tb00047.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 9
    Electronic Resource
    Electronic Resource
    Zur Analyse der Bewegungsempfindungen. Briefe von Josef Breuer an Ernst Mach. (1975)
    Oxford, UK : Blackwell Publishing Ltd
    Centaurus 19 (1975), S. 0 
    Details
    ISSN: 1600-0498
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine , Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0498.1975.tb00311.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 10
    Electronic Resource
    Electronic Resource
    Bone marrow histopathology following cytoreductive therapy in chronic idiopathic myelofibrosis (2003)
    Oxford, UK : Blackwell Science Ltd
    Histopathology 43 (2003), S. 0 
    Details
    ISSN: 1365-2559
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Aims:  To analyse systematically therapy-induced lesions of haematopoiesis in chronic idiopathic myelofibrosis (IMF).Methods and results:  A total of 759 sequential bone marrow (BM) biopsies (median interval 32 months) were performed in 261 patients with IMF. Besides a control group (symptomatic treatment), monotherapies included busulfan, hydroxyurea and interferon. In all therapy groups hypoplasia of varying degree was a frequent finding and often accompanied by a patchy distribution of haematopoiesis. Most conspicuous was gelatinous oedema showing a tendency to develop discrete reticulin fibrosis (scleroedema). Minimal to moderate maturation defects of megakaryopoiesis and erythroid precursors occurred, but overt myelodysplastic features were most prominent following hydroxyurea and busulfan therapy. Acceleration and blastic crisis were characterized by the appearance of immature and CD34+ progenitor cells. Concerning the dynamics of fibrosis, no differences were observed between controls and the various therapy groups. In 143 patients (55%) without or with little reticulin at onset, an increase in myelofibrosis was detectable that progressed to overt collagen fibrosis.Conclusions:  Therapy-related bone marrow lesions in IMF comprise a strikingly variable spectrum that may include aplasia with scleroedema and a patchy distribution of myelodysplastic haematopoiesis associated with progressive myelofibrosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2559.2003.01732.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...