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  • 1980-1984  (2)
  • Basolateral HCO 3 − transport  (1)
  • Hämoglobingehalt im Blut, kolorimetrische Eisenbestimmung  (1)
  • 1
    ISSN: 1437-1596
    Keywords: Colorimetric iron determination ; Hemoglobin content in blood, colorimetric iron determination ; Eisenbestimmung, kolorimetrisch ; Hämoglobingehalt im Blut, kolorimetrische Eisenbestimmung
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Description / Table of Contents: Zusammenfassung Es wird eine einfache und genaue Methode zur Bestimmung von Eisen im Blut unter Verwendung von Säureaufschluß im Heizblock beschrieben. Der Aufschluß benötigt wenig Mühe und erlaubt die Verarbeitung einer großen Zahl von Proben. Der verdünnte Aufschluß wird zur kolorimetrischen Eisenbestimmung mittels o-Phenanthrolin benutzt und die entsprechende Hämoglobinkonzentration im Blut berechnet. Es zeigte sich eine gute Korrelation der Ergebnisse mit der beschriebenen und der Cyanmethämoglobin-Methode anhand von 21 Standardproben, die 4,9 bis 26,8 g/dl Hämoglobin enthielten.
    Notes: Summary A simple and accurate determination of iron in blood involving acid digestion in a heating block is described. The digestion requires little attention and permits the handling of large numbers of samples. The diluted digest is used for the colorimetric determination of iron using o-phenanthroline, and the equivalent hemoglobin in blood is calculated. There was a good correlation between the results of the present method and the cyanmethemoglobin method with 21 standard blood samples containing 4.9–26.8 g/dl of hemoglobin.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-2013
    Keywords: Basolateral HCO 3 − transport ; pH ; $$p_{{\text{CO}}_{\text{2}} } $$ ; Carbonic anhydrase ; Anion substitution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The membrane potential response of proximal tubular cells to changing HCO 3 − concentrations was measured in micro-puncture experiments on rat kidney in vivo. No significant effect was noticed when luminal bicarbonate concentration was changed. Changing peritubular HCO 3 − by substitution with Cl− resulted in conspicuous membrane potential transients, which reached peak values after 100–200 ms and decayed towards near control with time constants of ∼2s. The polarity of the potential changes and the dependence of the initial potential deflections on the logarithm of HCO 3 − concentration suggest a high conductance of the peritubular cell membrane for HCO 3 − buffer, but not for Cl−, SO 4 2− , or isethionate. At constant pH $$t_{{\text{HCO}}_{\text{3}}^ - } $$ was estimated to amount to ∼0.68. At constant $$p_{{\text{CO}}_{\text{2}} } $$ , $$t_{{\text{HCO}}_{\text{3}}^ - } $$ was even greater because of an additional effect of OH− or respectively H+ gradients across the cell membrane. The secondary repolarization may be explained by passive net movements of K+ and HCO 3 − across the peritubular cell membrane, which result in a readjustment of intracellular HCO 3 − to the altered peritubular HCO 3 − concentration. Application of carbonic anhydrase inhibitors in the tubular lumen reduced the initial potential response by one half and doubled the repolarization time constant. The same effect occurred instantaneously when the inhibitor was applied—together with the HCO 3 − concentration step—in the peritubular perfusate. This observation demonstrates that membrane bound carbonic anhydrase is somehow involved in passive rheogenic bicarbonate transfer across the peritubular cell membrane, and suggests that HCO 3 − permeation might occur in form of CO2 and OH− (or H+ in opposite direction).
    Type of Medium: Electronic Resource
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