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  • 1980-1984  (2)
  • Budding bacteria  (1)
  • Synchronization technique  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Continuous culture and synchronization ofHyphomicrobium sp. B-522 (1982)
    Springer
    Archives of microbiology 132 (1982), S. 96-99 
    Details
    ISSN: 1432-072X
    Keywords: Hyphomicrobium ; Continuous culture ; Synchronization technique ; Synchronous swarmer cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A technique was developed for synchronization ofHyphomicrobium sp. strain B-522. Bacteria were grown in continuous culture with methanol (0.1%; v/v) growth limiting. Vitamin B12 (2.5 μg/l) was necessary to obtain steady state growth. The critical dilution rate wasD c =0.112; maximum cell output occurred atD=0.105 (Dx=30 mg l-1 h-1). Continuous cultures ofHyphomicrobium B-522 atD=0.110 were used to obtain cells for synchronization experiments. Synchronization was achieved by trapping young hyphal and budding cells in a glass wool column, while the initial swarmer cells were allowed to pass through. By semicontinuously rinsing the system, newly produced swarmers could be sampled in the effluent. The mean length of these synchronous swarmer cells was 1.25 μm (s=±0.13 μm; range 0,6 μm) as compared to 1.40 μm (s=±0.21 μm; range 1.2 μm) for swarmer cells of the continuous culture. Division of synchronous swarmer populations was completed after 7 h; the synchronization index was 0.76.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00690826
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Cell wall studies on budding bacteria of the Planctomyces/Pasteuria group and on a Prosthecomicrobium sp. (1984)
    Springer
    Archives of microbiology 138 (1984), S. 200-205 
    Details
    ISSN: 1432-072X
    Keywords: Budding bacteria ; Cell wall composition ; Antibiotic resistance ; Murein ; Taxonomy of eubacteria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Seven strains of budding, non-prosthecate bacteria belonging to the Planctomyces/Pasteuria group and a Prosthecomicrobium sp. were examined for muramic and diaminopimelic acids. These typical components of Gram-negative murein were found only in Prosthecomicrobium strain IFAM 1314, but they could not be detected in seven budding bacteria. Electron micrographs of ultrathin cell wall sections of strains IFAM 1313 and 1317 showed a membrane with bilayer structure outside the cytoplasmic membrane. 10% sodium dodecylsulfate treatment (30 min, 100°C) allowed the isolation of highly stable cell sacculi which, upon chemical analysis, proved to be mainly proteinaceous. The budding bacteria also showed considerable resistance against penicillin G, ampicillin, cephalotin and D-cycloserin. Our data indicate that these bacteria lack an ordinary Gram-negative type of murein and, instead, carry a stable protein envelope.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00402120
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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