ISSN:
1437-160X
Keywords:
Mononuclear phagocytes
;
Rheumatoid arthritis
;
Osteoarthritis
;
Enzyme histochemical demonstration of lysozyme
;
Polymorphonuclear cells
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Lysozyme-producing cells were analysed by enzyme histochemistry in paraffin sections of synovial tissue of 60 patients with rheumatoid arthritis (RA) and 20 patients with osteoarthritis (OA). For lysozyme detection three enzym histochemical systems — peroxydase-antiperoxydase, alkaline phosphatase and biotin-avidin — were used in parallel experiments. Lysozyme was found to be produced by polymorphonuclear cells, mononuclear phagocytes and part of synovial lining cells. All types of lysozyme-producing cells were increased in RA compared with OA. Subgrouping of RA synovitis according to histomorphological criteria allowed the demonstration of an inverse relationship between the number of lysozyme-producing cells and the grade of proliferation of fibroblasts, called mesenchymoid transformation by Fassbender [19]. The different methods of lysozyme detection differed in specificity and sensitivity. The immunoenzymatic staining of lysozyme allows specific and quantitative evaluation of phagocyting cells in RA and OA.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00541270
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