Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • 1980-1984  (2)
  • Carbamate herbicide  (1)
  • Dimethyl sulfoxide  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Binucleation and abnormal chromosome distribution inEuglena gracilis cells treated with dimethyl sulfoxide (1983)
    Springer
    Protoplasma 114 (1983), S. 62-66 
    Details
    ISSN: 1615-6102
    Keywords: Dimethyl sulfoxide ; Euglena gracilis ; Binucleation ; Karyological abnormalities
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The addition of 5% of dimethyl sulfoxide (DMSO) to cultures ofEuglena gracilis in the logarithmic phase of growth caused an immediate inhibition of cell multiplication and motility without completely blocking nuclear division. Importantly, some 50% of the cells were 2–3 times larger than normal and were also binucleate after 24–48 hours of treatment. Evidently binucleation resulted from the lack of cytokinesis after mitosis was induced. Transmission electron microscopy, using serial sections, showed the occurrence of nuclei either with a normal or a reduced number of chromatin masses. Solvent withdrawal led to a rapid recovery of all the normal cell activities. On the contrary, 2.5% of DMSO produced no effect during the entire period of treatment (48 hours), whereas a 1-hour exposure to 10% of the solvent was sufficient to provoke aspecific and irreversible cellular damage. Since DMSO is known to produce alterations in actin-containing structures in a wide variety of cells types, an involvement of microfilaments in cell motility, cytokinesis and chromosome separation during mitosis inEuglena is proposed and discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01279869
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Structural alterations inEuglena gracilis exposed to the herbicide isopropyl-N-phenylcarbamate (IPC) (1982)
    Springer
    Protoplasma 111 (1982), S. 189-194 
    Details
    ISSN: 1615-6102
    Keywords: Carbamate herbicide ; Euglena gracilis ; Nucleus ; Cytoskeleton ; Ultrastructural alterations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Ultrastructural abnormalities of various kinds and severity appeared inEuglena gracilis green cells after a 48-hour exposure to 4 × 10−4M of isoprophyl-N-phenylcarbamate (IPC), a widely used herbicide thought to affect microtubules and/or microtubule organizing centers in susceptible plant and animal species. A high proportion of cells contained nuclei in the G 2 phase of the cycle; in a significant percentage of organisms, however, structural aberrations of the nucleolus and chromosomes were evident. The pellicle outline, chloroplasts, mitochondria, and dictyosomes were also affected. The cytoplasm was rich in dense bodies which sometimes showed granular, fibrillar or tubular materials. Furthermore IPC partially inhibited flagellum regeneration after mechanical amputation. The mechanism by which IPC causes these responses remains unclear. Nevertheless, some indications suggest that the herbicide acts primarily on microtubule organizing centers. However, mitochondria, chloroplasts and nuclear constituents appear as possible additional targets of the compound.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01281966
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...