ISSN:
1432-072X
Keywords:
Photosynthesis
;
Chromatium vinosum
;
Thiosulfate
;
Cytochrome c
;
Oxidoreductase
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Chromatium vinosum cells form a vesicular type intracytoplasmic membrane system during phototrophic growth on thiosulfate.—An enzyme protein transferring electrons from thiosulfate to cytochromes of type c was enriched from S-144. The colorless thiosulfate: cytochrome c oxidoreductase was characterized by a molecular weight of 36,000 (after dodecylsulfate treatment) and 35,000 (by gel filtration). Isoelectric focusing revealed a pI range of 4.4 to 4.7. Apparent K m values for the cytochromes tested were in the μM range. — The endogenous electron acceptor compound, isolated from the chromatophore fraction P-144, was found to be a membrane-bound cytochrome c-552. The homogeneous cytochrome protein had an average pI value of 4.65 and a molecular weight of 71,500 determined by gel filtration. By dodecylsulfate electrophoresis it was cleaved into two proteins representing particle weights of 45,000 and 20,000.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00425951
Permalink
