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  • 1
    Electronic Resource
    Electronic Resource
    Biochemical Characterization of Two Tobacco Cell Lines With Different Levels of Cinnamoyl Putrescines (1982)
    s.l. : American Chemical Society
    Journal of natural products 45 (1982), S. 83-87 
    Details
    ISSN: 1520-6025
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/np50019a008
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  • 2
    Electronic Resource
    Electronic Resource
    The enzymatic system thiosulfate: Cytochrome c oxidoreductase from photolithoautotrophically grown Chromatium vinosum (1981)
    Springer
    Archives of microbiology 130 (1981), S. 334-338 
    Details
    ISSN: 1432-072X
    Keywords: Photosynthesis ; Chromatium vinosum ; Thiosulfate ; Cytochrome c ; Oxidoreductase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Chromatium vinosum cells form a vesicular type intracytoplasmic membrane system during phototrophic growth on thiosulfate.—An enzyme protein transferring electrons from thiosulfate to cytochromes of type c was enriched from S-144. The colorless thiosulfate: cytochrome c oxidoreductase was characterized by a molecular weight of 36,000 (after dodecylsulfate treatment) and 35,000 (by gel filtration). Isoelectric focusing revealed a pI range of 4.4 to 4.7. Apparent K m values for the cytochromes tested were in the μM range. — The endogenous electron acceptor compound, isolated from the chromatophore fraction P-144, was found to be a membrane-bound cytochrome c-552. The homogeneous cytochrome protein had an average pI value of 4.65 and a molecular weight of 71,500 determined by gel filtration. By dodecylsulfate electrophoresis it was cleaved into two proteins representing particle weights of 45,000 and 20,000.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425951
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  • 3
    Electronic Resource
    Electronic Resource
    Oxidative phosphorylation in Micrococcus denitrificans under autotrophic growth conditions (1971)
    Springer
    Archives of microbiology 76 (1971), S. 114-125 
    Details
    ISSN: 1432-072X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Cell-free preparations from autotrophically grown M. denitrificans yielded P/O ratios of 0.6–1.6 with H2, 0.4–1.2 with NADH, 0.7–1.0 with succinate, and 0.3–0.5 with ascorbate as the oxidizable substrates. The phosphorylation in all cases was inhibited effectively by DNP, DBP, PCP, CCCP, and dicumarol at concentrations which did not cause any significant inhibition of oxygen uptake. The respiratory chain inhibitors such as antimycin A, HOQNO, cyanide, and azide were the potent inhibitors of the phosphate esterification coupled to the oxidation of H2, NADH, and succinate; the ascorbate-linked phosphorylation was inhibited by cyanide or azide only. While the NADH oxidation and associated phosphorylation was markedly sensitive to rotenone and other flavoprotein inhibitors, the oxidation of H2 was relatively insensitive although there was a partial inhibition of the coupled phosphorylation. The experimental results indicated that bulk of the electron transfer from H2 bypassed the NADH-dehydrogenase or the rotenone sensitive site.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00411785
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  • 4
    Electronic Resource
    Electronic Resource
    Thiosulfate-linked ATP-dependent NAD+ reduction in Rhodopseudomonas palustris (1971)
    Springer
    Archives of microbiology 80 (1971), S. 97-114 
    Details
    ISSN: 1432-072X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A cytochrome containing fraction virtually devoid of the photosynthetic apparatus (bacteriochlorophyll and/or chromatophores) was isolated from Rps. palustris grown photolithotrophically with S2O3 =as the exogenous electron donor. This fraction contained predominantly cytochromes of c, a and o type and exhibited thiosulfate: cytochrome c oxidoreductase and ferrocytochrome c:O2 oxidoreductase activities. Under anaerobic conditions the enzyme preparation catalyzed an ATP-dependent NAD+ reduction by S2O3 =in the dark involving a reversal of electron transfer from cytochrome c and yielding a molar stoichiometry of approximately 2:1 for the ferrocytochrome c oxidized and NAD+ reduced. In this process approximately 4 to 7 molar equivalents of ATP were utilized/equivalent of NAD+ reduced. The optimal reaction occurred at pH 8.0 and in the presence of 55 μM added mammalian cyt. c, 1.7 mM Mg++, 1.7 mM ATP and 7.0 mM S2O3 =. The S2O3 =-linked ATP-driven reduction of NAD+ as well as the coupled oxidation of cyt. c were inhibited completely by 5 μm CCCP or 10 μM DNP and the reaction was also markedly sensitive to other uncouplers of the energy transfer reactions. The pathway of electron transfer from S2O3 = to NAD+ appears to involve cyt. c, b, and flavoprotein systems as evidenced by the complete inhibition of the process by low concentrations of antimycin A, NOQNO, rotenone and amytal.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00411876
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  • 5
    Electronic Resource
    Electronic Resource
    The enzymatic system thiosulfate: Cytochrome c oxidoreductase from photolithoautotrophically grown Rhodopseudomonas palustris (1981)
    Springer
    Archives of microbiology 130 (1981), S. 328-333 
    Details
    ISSN: 1432-072X
    Keywords: Photosynthesis ; Rhodopseudomonas palustris ; Thiosulfate ; Cytochrome c ; Oxidoreductase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Rhodopseudomonas palustris cells, characterized by a lamellar type intracytoplasmic chromatophore membrane system after phototrophic growth, yielded a crude supernatant cell-free fraction (S-144) after ultracentrifugation which retained the contents of both the cell compartments. After thiosulfate-dependent growth, a protein system was isolated from S-144 which catalyzed the thiosulfate-linked reduction of an endogenous c-type cytochrome. — The colorless oxidoreductase protein, after purification to homogeneity, revealed a molecular weight of 93,000 and, after SDS treatment, a particle weight of 48,000. It was focused at an average pI of 5.45. Apparent K m values for several substrates were in the μM range. The electron acceptor for thiosulfate oxidation was found to be a cytochrome c from S-144. The homogeneous acceptor protein, at liquid nitrogen temperature, exhibited absorption maxima at 549.0, 518.5 and 418.0 nm, and shoulders at 525.5, 512.0 and 508.0 nm. Its molecular weight was found to be 17,000 (gel filtration) and 16,000 (SDS gel electrophoresis). It was characterized by a pI of 10.0. Its midpoint redox potential of E m,7.0=+228 mV was determined by redox titrations and the value of +205 mV by spectrophotometric calculations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425950
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  • 6
    Electronic Resource
    Electronic Resource
    Purification and properties of the adenylate kinases from Rhodopseudomonas palustris, Rhodopseudomonas sphaeroides and Rhodopseudomonas rubrum (1983)
    Springer
    Archives of microbiology 134 (1983), S. 153-157 
    Details
    ISSN: 1432-072X
    Keywords: Adenylate kinase ; Chromatophores ; Membranes ; Photosynthesis ; Rhodopseudomonas ; Rhodospirillum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The adenylate kinases (EC 2.7.4.3) from photosynthetically grown Rhodopseudomonas palustris, Rhodopseudomonas sphaeroides and Rhodospirillum rubrum were purified to homogeneity by the same procedure. The purified enzymes showed optimal rates of activity with MgCl2 at 25° C and pH 8.0. They were found to be heat labile and were characterized by pI-values of 4.5. Apparent molecular weights of 33 500 for R. palustris, 34 400 for R. sphaeroides and 32 100 for R. rubrum were determined by high performance liquid chromatography. No separation into subunits was observed by use of sodium dodecylsulfate polyacrylamide gel electrophoresis. The apparent K m -values for ADP corresponded to 0.26 mM for R. palustris, 0.27 mM for R. sphaeroides and 0.24 mM for R. rubrum. ADP in excess had a strong inhibitory effect. Competitive product inhibition was found for AMP, with K i-values of 0.017 mM for R. palustris, 0.018 mM for R. sphaeroides and 0.014 mM for R. rubrum. A competitive inhibitor likewise was P1,P5-di(adenosine-5′)pentaphosphate with K i-values of 0.020 μM for R. palustris and R. sphaeroides, and 0.017 μM for R. rubrum. Sulfhydryl-reacting reagents like p-chloromercuribenzoate and iodoacetic acid were found to be non-inhibitory. All measurements of adenylate kinase activity were carried out with the stabilized and most sensitive luciferin-luciferase system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00407949
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  • 7
    Electronic Resource
    Electronic Resource
    Über ätherische Öle des Ingwer,Zingiber oficinalis Roscoe (1988)
    Springer
    European food research and technology 186 (1988), S. 231-234 
    Details
    ISSN: 1438-2385
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Description / Table of Contents: Zusammenfassung Die etherischen Öle aus Rhizomen von Ingwer (Zingiber officinalis Rosc.) indischer und australischer Herkunft unterscheiden sich grundlegend in ihrer Zusammensetzung. Hauptbestandteile des aus Indischem Ingwer gewonnenen Öls sind die Sesquiterpenkohlenwasserstoffe ar-Curcumen, Zingiberen, α-Farnesen,β-Bisabolen undβ-Sesquiphel-landren. Hingegen besteht das etherische Öl des Australischen Ingwwes hauptsächlich aus den Monoterpenkohlenwasserstoffen Camphen und Phellandren und deren sauerstoffhaltigen Derivaten Neral, Geranial und 1,8-Cineol.
    Notes: Summary Essential oils from ginger rhizomes of different origins, such asZingiber officinalis Rosc. from India and Australia, differ remarkably in their terpenoid compositions. The main components of Indian ginger oil are the sesquiterpenoid hydrocarbons, arcurcumene, zingiberene, α-farnesene,β-bisabolene andβ-sesquiphellandrene. However, the essential oil from the Australian ginger consists mainly of the monoterpenoid hydrocarbons, camphene and phellandrene, and their oxygen-containing derivatives neral, geranial and 1,8-cineol.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01043318
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  • 8
    Electronic Resource
    Electronic Resource
    Inhibitory effects of essential oil components on growth of food-contaminating fungi (1987)
    Springer
    European food research and technology 185 (1987), S. 10-13 
    Details
    ISSN: 1438-2385
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Description / Table of Contents: Summary The antifungal activity of several components of essential oils were evaluated using a paper-disk method. The substances investigated are structurally related to eugenol. Equimolar amounts were tested on more than ten fungal strains known to contaminate food. Iso-eugenol, cinnamaldehyde, carvacrol, eugenol and thymol revealed the strongest antifungal activity. The most resistant strain appeared to bePenicillium verrucosum var.cyclopium, and the most sensitive wasP. viridicatum. Some of the structural effects were considered, including a free hydroxyl group[
    Notes: Zusammenfassung Die antimykotische Wirkung mehrerer Komponenten von ätherischen Ölen wurde mit einem Agar-Diffusionstest bestimmt. Die untersuchten Substanzen sind strukturell mit Eugenol verwandt. Äquimolare Mengen wurden an mehr als zehn Schimmelpilzstämmen getestet, von denen bekannt ist, daß sie Lebensmittel kontaminieren. Iso-Eugenol, Zimtaldehyd, Carvacrol, Eugenol und Thymol zeigten die stärkste antimykotische Wirkung. Der unempfindlichste Stamm war einPenicillium verrucosum var.cyclopium, während einP. viridicatum den sensibelsten darstellte. Einige Struktur-Wirkungsbeziehungen konnten in Betracht gezogen werden. Eine aktive Konfiguration bei phenolischen Verbindungen zur Entfaltung antimykotischer Wirkung scheint die freie Hydroxylgruppe in Verbindung mit einem Aklkylsubstituenten zu sein.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01083332
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  • 9
    Electronic Resource
    Electronic Resource
    Über die ätherischen Öle der Weinraute (Ruta graveolens L.) (1985)
    Springer
    European food research and technology 181 (1985), S. 28-31 
    Details
    ISSN: 1438-2385
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Description / Table of Contents: Zusammenfassung Der Gehalt an ätherischem Öl inRuta graveolens L. unterliegt einer jahreszeitlichen Abhängigkeit. Dies gilt für frische und getrocknete Blätter wie für die Blüten und Früchte. Getrocknete Blätter enthalten weniger ätherisches Öl insbesondere in Bezug auf 2-Nonanon. Im Vergleich der verschiedenen Pflanzenteile untereinander ist die Zusammensetzung des Öles der Blätter besonders komplex. In quantitativer Hinsicht enthalten Blätter halb so viel ätherisches Öl wie die Blüten und etwa ein Drittel vom festgestellten Gehalt in den Früchten. Die Hauptkomponenten des ätherischen Öles der Blätter sind 2-Nonanon, 2-Nonylacetat und 2-Undecylacetat. 2-Undecanon und 2-Nonanon sind die Hauptbestandteile des ätherischen Öles der Blüten und Früchte.[/p]
    Notes: Summary A seasonal dependence has been observed in the essential oil contents of fresh and dried leaves and, likewise, blossoms and fruits of Ruta graveolens L. Dried leaves are characterized by a decreased content of essential oil, especially of 2-nonanone. Of the different parts of the plant, the oil from the leaves had the most complex composition. Quantitatively, the leaves contained half the amount of oil if compared to the blossoms, and about one third of the amount present in fruits. The main components of the oil synthesized in leaves are 2-nonanone, 2-nonylacetate, and 2-undecylacetate; 2-undecanone and 2-nonanone are predominant in the oil from blossoms and fruits.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01124803
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  • 10
    Electronic Resource
    Electronic Resource
    Das ätherische Öl vonArtemisia abrotanum L. (1984)
    Springer
    European food research and technology 179 (1984), S. 125-128 
    Details
    ISSN: 1438-2385
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Description / Table of Contents: Zusammenfassung Das ätherische Öl der Blätter vonArtemisia abrotanum L. wurde mittels Trockensäulen-Chromatographie fraktioniert und etwa 40 unterschiedliche Komponenten mittels Gaschromatographie und Massenspektrometrie identifiziert. Der Gehalt an ätherischem Öl zeigte keine charakteristische jahreszeitliche Abhängigkeit.
    Notes: Summary The essential oil from leaves ofArtemisia abrotanum L. was fractionated by dry column chromatography. About 40 different components were identified by gas chromatography and mass spectrometry. The production of essential oil by the leaves did not show any distinct seasonal dependence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01043263
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