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  • 1
    Electronic Resource
    Electronic Resource
    Biochemical Characterization of Two Tobacco Cell Lines With Different Levels of Cinnamoyl Putrescines (1982)
    s.l. : American Chemical Society
    Journal of natural products 45 (1982), S. 83-87 
    Details
    ISSN: 1520-6025
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/np50019a008
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  • 2
    Electronic Resource
    Electronic Resource
    Uptake of phosphate and its effect on phenylalanine ammonia-lyase activity and cinnamoyl putrescine accumulation in cell suspension cultures of Nicotiana tabacum (1982)
    Springer
    Plant cell reports 1 (1982), S. 128-130 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The influence of phosphate on the medium-induced formation of cinnamoyl putrescines in cell cultures of Nicotiana tabcum was investigated. Phosphate added to a phosphate-free production medium was completely accumulated in the cells within 24h after inoculation at initial concentrations up to 2 mM. At higher concentrations phosphate was partly accumulated with an intracellular saturation at approx. 0.65 mmol/g dr. wt. equivalent to approx. 45 mM intracellular concentration. Enhanced activities of phenylalanine ammonialyase and increased product levels of cinnamoyl putrescines, induced by cell transfer into phosphatefree medium were suppressed similarly at initial phosphate concentrations of 0.02–0.5 mM. At the same time growth was stimulated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00272370
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  • 3
    Electronic Resource
    Electronic Resource
    Purification and properties of the adenylate kinases from Rhodopseudomonas palustris, Rhodopseudomonas sphaeroides and Rhodopseudomonas rubrum (1983)
    Springer
    Archives of microbiology 134 (1983), S. 153-157 
    Details
    ISSN: 1432-072X
    Keywords: Adenylate kinase ; Chromatophores ; Membranes ; Photosynthesis ; Rhodopseudomonas ; Rhodospirillum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The adenylate kinases (EC 2.7.4.3) from photosynthetically grown Rhodopseudomonas palustris, Rhodopseudomonas sphaeroides and Rhodospirillum rubrum were purified to homogeneity by the same procedure. The purified enzymes showed optimal rates of activity with MgCl2 at 25° C and pH 8.0. They were found to be heat labile and were characterized by pI-values of 4.5. Apparent molecular weights of 33 500 for R. palustris, 34 400 for R. sphaeroides and 32 100 for R. rubrum were determined by high performance liquid chromatography. No separation into subunits was observed by use of sodium dodecylsulfate polyacrylamide gel electrophoresis. The apparent K m -values for ADP corresponded to 0.26 mM for R. palustris, 0.27 mM for R. sphaeroides and 0.24 mM for R. rubrum. ADP in excess had a strong inhibitory effect. Competitive product inhibition was found for AMP, with K i-values of 0.017 mM for R. palustris, 0.018 mM for R. sphaeroides and 0.014 mM for R. rubrum. A competitive inhibitor likewise was P1,P5-di(adenosine-5′)pentaphosphate with K i-values of 0.020 μM for R. palustris and R. sphaeroides, and 0.017 μM for R. rubrum. Sulfhydryl-reacting reagents like p-chloromercuribenzoate and iodoacetic acid were found to be non-inhibitory. All measurements of adenylate kinase activity were carried out with the stabilized and most sensitive luciferin-luciferase system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00407949
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  • 4
    Electronic Resource
    Electronic Resource
    The enzymatic system thiosulfate: Cytochrome c oxidoreductase from photolithoautotrophically grown Rhodopseudomonas palustris (1981)
    Springer
    Archives of microbiology 130 (1981), S. 328-333 
    Details
    ISSN: 1432-072X
    Keywords: Photosynthesis ; Rhodopseudomonas palustris ; Thiosulfate ; Cytochrome c ; Oxidoreductase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Rhodopseudomonas palustris cells, characterized by a lamellar type intracytoplasmic chromatophore membrane system after phototrophic growth, yielded a crude supernatant cell-free fraction (S-144) after ultracentrifugation which retained the contents of both the cell compartments. After thiosulfate-dependent growth, a protein system was isolated from S-144 which catalyzed the thiosulfate-linked reduction of an endogenous c-type cytochrome. — The colorless oxidoreductase protein, after purification to homogeneity, revealed a molecular weight of 93,000 and, after SDS treatment, a particle weight of 48,000. It was focused at an average pI of 5.45. Apparent K m values for several substrates were in the μM range. The electron acceptor for thiosulfate oxidation was found to be a cytochrome c from S-144. The homogeneous acceptor protein, at liquid nitrogen temperature, exhibited absorption maxima at 549.0, 518.5 and 418.0 nm, and shoulders at 525.5, 512.0 and 508.0 nm. Its molecular weight was found to be 17,000 (gel filtration) and 16,000 (SDS gel electrophoresis). It was characterized by a pI of 10.0. Its midpoint redox potential of E m,7.0=+228 mV was determined by redox titrations and the value of +205 mV by spectrophotometric calculations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425950
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  • 5
    Electronic Resource
    Electronic Resource
    The enzymatic system thiosulfate: Cytochrome c oxidoreductase from photolithoautotrophically grown Chromatium vinosum (1981)
    Springer
    Archives of microbiology 130 (1981), S. 334-338 
    Details
    ISSN: 1432-072X
    Keywords: Photosynthesis ; Chromatium vinosum ; Thiosulfate ; Cytochrome c ; Oxidoreductase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Chromatium vinosum cells form a vesicular type intracytoplasmic membrane system during phototrophic growth on thiosulfate.—An enzyme protein transferring electrons from thiosulfate to cytochromes of type c was enriched from S-144. The colorless thiosulfate: cytochrome c oxidoreductase was characterized by a molecular weight of 36,000 (after dodecylsulfate treatment) and 35,000 (by gel filtration). Isoelectric focusing revealed a pI range of 4.4 to 4.7. Apparent K m values for the cytochromes tested were in the μM range. — The endogenous electron acceptor compound, isolated from the chromatophore fraction P-144, was found to be a membrane-bound cytochrome c-552. The homogeneous cytochrome protein had an average pI value of 4.65 and a molecular weight of 71,500 determined by gel filtration. By dodecylsulfate electrophoresis it was cleaved into two proteins representing particle weights of 45,000 and 20,000.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425951
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  • 6
    Electronic Resource
    Electronic Resource
    Über die Komponenten des atherischen Öls aus Estragon (Artemisia dracunculus L.) (1981)
    Springer
    European food research and technology 173 (1981), S. 365-367 
    Details
    ISSN: 1438-2385
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Description / Table of Contents: Zusammenfassung Im ätherischen Öl des Estragons (Artemisia dracunculus L.) wurden 30 Einzelkomponenten identifiziert. Als Hauptkomponenten fanden sich Sabinen, Methyleugenol, Estragol und Elemicin. Der Gehalt an ätherschm Öl zeigte eine charakteristische jahreszeitliche Abhängigkeit.
    Notes: Summary 30 compounds could be identified in the essential oil of tarragon (Artemisia dracunculus L.). The main components were sabinene, methyl eugenol, estragol and elemicine. The content of essential oil per unit dry weight had a characteristic seasonal dependence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01042057
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  • 7
    Electronic Resource
    Electronic Resource
    Das ätherische Öl vonArtemisia abrotanum L. (1984)
    Springer
    European food research and technology 179 (1984), S. 125-128 
    Details
    ISSN: 1438-2385
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Description / Table of Contents: Zusammenfassung Das ätherische Öl der Blätter vonArtemisia abrotanum L. wurde mittels Trockensäulen-Chromatographie fraktioniert und etwa 40 unterschiedliche Komponenten mittels Gaschromatographie und Massenspektrometrie identifiziert. Der Gehalt an ätherischem Öl zeigte keine charakteristische jahreszeitliche Abhängigkeit.
    Notes: Summary The essential oil from leaves ofArtemisia abrotanum L. was fractionated by dry column chromatography. About 40 different components were identified by gas chromatography and mass spectrometry. The production of essential oil by the leaves did not show any distinct seasonal dependence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01043263
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