ISSN:
1432-1912
Keywords:
Rat cerebral cortex
;
α1-Adrenoceptor
;
[3H]-WB4101 binding
;
Hyperthyroidism
;
Hypothyroidism
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary The influence of thyroid hormones on the concentration and properties of α1-adrenoceptors in a crude membrane fraction obtained from the rat cerebral cortex was investigated using the [3H]-WB 4101 binding assay. Animals were made hypothyroid by feeding 6-propyl-2-thiouracil for 8 weeks. Hyperthyroidism was induced by triiodothyronine injections (50 μg/100 g body weight) for 9 days. 1. The binding of [3H]-WB 4101 was saturable and of high affinity in controls as well as in hyper- and hypothyroid animals. The maximal number of binding sites (B max), which amounted to 95 fmol/mg protein in control animals, was increased by 27% in cortical membranes from hyperthyroid rats and reduced by 23% in the hypothyroid group. 2. The reduction in [3H]-WB 4101 binding due to 6-propyl-2-thiouracil feeding was reversible by triiodothyronine treatment. 3. Dissociation constants (K D) calculated from saturation experiments (0.25 nM) or kinetic data (0.21 nM) remained unchanged in altered thyroid states. 4. Inhibition of [3H]-WB 4101 binding by adrenergic agonists and antagonists revealed no differences between euthyroid and hypothyroid animals. The higher affinity of prazosin to the binding sites compared with yohimbine indicated that [3H]-WB 4101 predominantly labeled α1-adrenoceptors. It is concluded that thyroid hormones regulate the number of α1-adrenoceptors in membranes of the rat cerebral cortex, leaving their affinities unchanged.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00507226
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