ISSN:
1432-1440
Keywords:
Luminescence
;
Blood bactericidal activity
;
Phagocytosis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary To evaluate phagocytosis of granulocyte, chemiluminescence (CL) — measurements were done in whole blood specimen. By this method much less variation (22% versus 84%) could be achieved in the whole blood specimen compared with isolated granulocytes. The quenching of CL by erythrocytes, which is dependent on the quotient erythrocytes/granulocytes, could be significantly reduced by dilution (1:5,000). Optimized reaction conditions were selected (approx. 5 × more sensitive then previous protocols) to evaluate the phagocytic activity of the few remaining granulocytes (approx. 2,000/ml) with routine luminometers.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01486371
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