ISSN:
1432-1106
Keywords:
Intrinsic cholinergic excitation
;
Nicotinic receptors
;
Fast excitation
;
Muscarinic receptors
;
Slow excitation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary An in vitro slice technique was employed to study the receptors involved in intrinsic cholinergic excitation in the rat neostriatum. The locally evoked synaptic potentials were suppressed by antinicotinic agents, mecamylamine (10 μM), d-tubocurarine (3 μM) or hexamethonium (100 μM), but not by the antimuscarinic agent atropine (100 μM). If the slices were exposed to an acetylcholinesterase (AChE)-inhibitor (paraoxon 1–20 μM, physostigmine 0.1–0.5 μM), the synaptic potentials were potentiated. The amplitude of the orthodromic population spike increased, and it was further facilitated when the stimulus frequencies were raised from 1–3 Hz to 10–30 Hz. The frequency facilitation following exposure to an AChE-inhibitor was blocked by atropine (1–100 μM). Intracellular recording indicated that a slow depolarizing potential caused the frequency potentiation of the orthodromic discharges. Apparently rat neostriatum is similar to cholinergic systems in sympathetic ganglia and spinal Renshaw cells, in that nicotinic receptors mediate fast excitation and muscarinic receptors mediate slow excitation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00239304
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