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  • 1980-1984  (2)
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  • 1
    Electronic Resource
    Electronic Resource
    Isolation of the pMI 10 plasmid from the α-amylase producing strain of Bacillus subtilis (1981)
    Springer
    Molecular genetics and genomics 181 (1981), S. 248-253 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary B. subtilis A 18, a producer of exocellular amylase, was found to carry covalently closed DNA plasmid molecules (pMI 10). The pMI 10 was isolated and characterized by electron microscopy, electrophoretic mobility and restriction endonuclease cleavage pattern. The pMI 10 was absent in all α-amylase low productive or nonproductive clones. The pMI 10 DNA was transformed together with pUB 110 DNA into B. subtilis RM 125 arg-leu- recipient cells, and, hence, compatibility of these plasmids could be demonstrated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00268433
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Physical characterization of the plasmid pON5300 (1981)
    Springer
    Molecular genetics and genomics 184 (1981), S. 107-110 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The antibiotic resistance plasmid Rldrd19Km-which has spontaneously lost its kanamycin resistance marker, and its derivative pON5300, were analysed using the restriction endonucleases SalI, BamHI, HindIII and EcoRI. The fragment patterns were compared with that of the Rldrd19 and the fragments responsible for kanamycin resistance were found to be missing in Rldrd19Km − and pON5300. In these plasmids a 7 Mg/mol EcoRI fragment was observed instead of the D (6.3 Mg/mol) fragment of Rldrd19. Further a new 6 Mg/mol EcoRI restriction fragment was observed in pON5300. Using double digestions it was shown that the new fragment does not carry restriction sites for HindIII, BamHI and SalI endonucleases. The non-homology of the analysed plasmid was proved electron microscopically by heteroduplex techniques. The possibility of amplification in the regulatory region for the expression of R-determinants in pON5300 is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00271204
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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