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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 133 (1982), S. 28-32 
    ISSN: 1432-072X
    Keywords: Uranium oxidation ; Iron oxidation ; Thiobacillus ferrooxidans ; Carbon dioxide fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The stoichiometric oxidation of uranous-to uranyl-uranium byThiobacllus ferrooxidans is demonstrated. Fixation of14CO2 and the effect of inhibitors demonstrate that energy is conserved during the oxidation and used for energy-dependent “reverse electron flow” and carbon dioxide fixation.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 133 (1982), S. 33-37 
    ISSN: 1432-072X
    Keywords: Uranium oxidation ; Iron oxidation ; Oxygen uptake ; Carbon dioxide fixation ; Uranium toxicity ; Thorium toxicity ; Thiobacillus ferrooxidans
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Kinetic constants for the oxidation of uranous and ferrous ions byThiobacillus ferrooxidans were estimated. The kinetics indicate a direct biological mechanism for uranium oxidation. The complex interrelations of ferric, uranyl and uranous ion inhibition are considered.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-072X
    Keywords: Ubiquinone ; Isoprenoid quinone ; Quinone composition ; Thiobacillus ferrooxidans ; Mass spectrometry ; High performance liquid chromatography ; Thin layer chromatography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A method was developed for the isolation of bacterial isoprenoid quinones. This method gave good yields and was superior to two standard methods that were also tested, because the cell membrane isolation and methanol extraction steps could be eliminated. The ubiquinone composition of Thiobacillus ferrooxidans was analyzed; only ubiquinone-8 was detected and its identification was resolved by thin layer chromatography, high performance liquid chromatography, and mass spectrometry.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 135 (1983), S. 250-253 
    ISSN: 1432-072X
    Keywords: Uranium ; Thiobacilli ; Cell fractionation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The uptake and cellular distribution of UO 2 2+ were investigated in washed cell suspensions of Thiobacillus ferrooxidans. The uptake was dependent on external concentration of uranium (0.01–10.0 mM) and was influenced by the pH of the reaction mixture, but not by 1 mM transition metals ions or by the previous growth history of the cells. Cells inactivated by either ultraviolet radiation or potassium cyanide accumulated about 40% more uranium than did viable cells especially at a high, toxic UO 2 2+ concentration. Most of the uranium was associated with the cell wall and membrane fractions and relatively little uranium was detected in the cytoplasmic, lipopolysaccaride and periplasmic space material fractions. Cells poisoned with potassium cyanide were found to have an 8 to 11-fold increase in the cytoplasmic concentration of uranium.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 25 (1983), S. 1163-1168 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 23 (1981), S. 2761-2769 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Particulate materials (flowers of sulfur, fluorapatite, glass beads, and pyrite) inhibited the growth of Thiobacillus ferrooxidans on ferrous iron at pH 1.5. The degree of inhabitation varied with the type of the particulate and culture conditions. The inhibition caused by pyrite involved a soluble toxic component. The inhibition caused by sulfur particles and glass beads was alleviated by incubation under static culture conditions (i.e., no shaking) but oxidation rates declined because of lack of aeration an increase in the pH above 2 also relieved the inhibition. The nature of inhibition. The nature of inhibition by different particles varied from declined iron oxidation rates to prolonged lag periods depending on the concentration of the test material. Thus, the results indicate that the bacterial activity at the liquid-solid interface is different from that in the bulk liquid phase and is influenced by the proton concentration and by the physiochemical properties of the solids.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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